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[Stress-responsive expression analysis of glutathione-S-transferase gene of Ipomoea batatas (L.) Lam].

A prokaryotic expression plasmid pET-IbGST, which contains the full encoding region of a glutathione-S- transferase (GST) gene of sweet potato, was constructed. The recombinant IBGSTU1 protein was expressed in Escherichia coli and found in the soluble fraction, as well as in insoluble inclusion bodi... Full description

Journal Title: Yi chuan = Hereditas August 2009, Vol.31(8), pp.859-864
Main Author: Liu, Xun
Other Authors: He, Bo-Wen , Zhang, Yi-Zheng
Format: Electronic Article Electronic Article
Language: chi
Subjects:
ID: ISSN: 0253-9772
Link: http://search.proquest.com/docview/67594456/?pq-origsite=primo
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title: [Stress-responsive expression analysis of glutathione-S-transferase gene of Ipomoea batatas (L.) Lam].
format: Article
creator:
  • Liu, Xun
  • He, Bo-Wen
  • Zhang, Yi-Zheng
subjects:
  • Escherichia Coli–Genetics
  • Gene Expression–Metabolism
  • Gene Expression Regulation, Plant–Genetics
  • Glutathione Transferase–Isolation & Purification
  • Ipomoea Batatas–Metabolism
  • Plant Proteins–Enzymology
  • Recombinant Fusion Proteins–Genetics
  • Stress, Physiological–Physiology
  • Stress, Physiological–Genetics
  • Stress, Physiological–Isolation & Purification
  • Stress, Physiological–Metabolism
  • Stress, Physiological–Genetics
  • Stress, Physiological–Metabolism
  • Plant Proteins
  • Recombinant Fusion Proteins
  • Glutathione Transferase
ispartof: Yi chuan = Hereditas, August 2009, Vol.31(8), pp.859-864
description: A prokaryotic expression plasmid pET-IbGST, which contains the full encoding region of a glutathione-S- transferase (GST) gene of sweet potato, was constructed. The recombinant IBGSTU1 protein was expressed in Escherichia coli and found in the soluble fraction, as well as in insoluble inclusion bodies of lysed cells. Its enzymatic activity was detected using UV spectrophotometer. The protein was purified and used to prepare antibody. Semi-quantitative RT-PCR and Western blotting analyses demonstrated that IBGSTU1 gene was not expressed under normal conditions. When subjected to some environ-mental stresses such as cold-stress or heavy-medal stress, the organism switches on the expression of IBGSTU1 at both mRNA and protein levels, and its expression has tissue specificity.
language: chi
source:
identifier: ISSN: 0253-9772
fulltext: no_fulltext
issn:
  • 02539772
  • 0253-9772
url: Link


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title[Stress-responsive expression analysis of glutathione-S-transferase gene of Ipomoea batatas (L.) Lam].
creatorLiu, Xun ; He, Bo-Wen ; Zhang, Yi-Zheng
contributorLiu, Xun (correspondence author) ; Liu, Xun (record owner)
ispartofYi chuan = Hereditas, August 2009, Vol.31(8), pp.859-864
identifierISSN: 0253-9772
subjectEscherichia Coli–Genetics ; Gene Expression–Metabolism ; Gene Expression Regulation, Plant–Genetics ; Glutathione Transferase–Isolation & Purification ; Ipomoea Batatas–Metabolism ; Plant Proteins–Enzymology ; Recombinant Fusion Proteins–Genetics ; Stress, Physiological–Physiology ; Stress, Physiological–Genetics ; Stress, Physiological–Isolation & Purification ; Stress, Physiological–Metabolism ; Stress, Physiological–Genetics ; Stress, Physiological–Metabolism ; Plant Proteins ; Recombinant Fusion Proteins ; Glutathione Transferase
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descriptionA prokaryotic expression plasmid pET-IbGST, which contains the full encoding region of a glutathione-S- transferase (GST) gene of sweet potato, was constructed. The recombinant IBGSTU1 protein was expressed in Escherichia coli and found in the soluble fraction, as well as in insoluble inclusion bodies of lysed cells. Its enzymatic activity was detected using UV spectrophotometer. The protein was purified and used to prepare antibody. Semi-quantitative RT-PCR and Western blotting analyses demonstrated that IBGSTU1 gene was not expressed under normal conditions. When subjected to some environ-mental stresses such as cold-stress or heavy-medal stress, the organism switches on the expression of IBGSTU1 at both mRNA and protein levels, and its expression has tissue specificity.
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title[Stress-responsive expression analysis of glutathione-S-transferase gene of Ipomoea batatas (L.) Lam].
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