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A proteome reference map and proteomic analysis of Bifidobacterium longum NCC2705.

A comprehensive proteomic study was carried out to identify and characterize proteins expressed by Bifidobacterium longum NCC2705. A total of 708 spots representing 369 protein entries were identified by MALDI-TOF-MS and/or ESI-MS/MS. Isoelectric point values estimated by gel electrophoresis matched... Full description

Journal Title: Molecular & cellular proteomics : MCP June 2006, Vol.5(6), pp.1105-1118
Main Author: Yuan, Jing
Other Authors: Zhu, Li , Liu, Xiankai , Li, Ting , Zhang, Ying , Ying, Tianyi , Wang, Bin , Wang, Junjun , Dong, Hua , Feng, Erling , Li, Qiang , Wang, Jie , Wang, Hongxia , Wei, Kaihua , Zhang, Xuemin , Huang, Cuifeng , Huang, Peitang , Huang, Liuyu , Zeng, Ming , Wang, Hengliang
Format: Electronic Article Electronic Article
Language: English
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ID: ISSN: 1535-9476
Link: http://search.proquest.com/docview/68078295/?pq-origsite=primo
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title: A proteome reference map and proteomic analysis of Bifidobacterium longum NCC2705.
format: Article
creator:
  • Yuan, Jing
  • Zhu, Li
  • Liu, Xiankai
  • Li, Ting
  • Zhang, Ying
  • Ying, Tianyi
  • Wang, Bin
  • Wang, Junjun
  • Dong, Hua
  • Feng, Erling
  • Li, Qiang
  • Wang, Jie
  • Wang, Hongxia
  • Wei, Kaihua
  • Zhang, Xuemin
  • Huang, Cuifeng
  • Huang, Peitang
  • Huang, Liuyu
  • Zeng, Ming
  • Wang, Hengliang
subjects:
  • ATP-Binding Cassette Transporters–Metabolism
  • Amino Acids–Analysis
  • Bacterial Proteins–Chemistry
  • Bifidobacterium–Genetics
  • Electrophoresis, Gel, Two-Dimensional–Metabolism
  • Energy Metabolism–Metabolism
  • Fructose–Metabolism
  • Glucose–Analysis
  • Glycolysis–Metabolism
  • Heat-Shock Proteins–Analysis
  • Nucleotides–Analysis
  • Open Reading Frames–Analysis
  • Proteome–Analysis
  • Proteomics–Analysis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization–Analysis
  • ATP-Binding Cassette Transporters
  • Amino Acids
  • Bacterial Proteins
  • Heat-Shock Proteins
  • Nucleotides
  • Proteome
  • Fructose
  • Glucose
ispartof: Molecular & cellular proteomics : MCP, June 2006, Vol.5(6), pp.1105-1118
description: A comprehensive proteomic study was carried out to identify and characterize proteins expressed by Bifidobacterium longum NCC2705. A total of 708 spots representing 369 protein entries were identified by MALDI-TOF-MS and/or ESI-MS/MS. Isoelectric point values estimated by gel electrophoresis matched closely with their predicted ones, although some discrepancies exist suggesting that post-translational protein modifications might be common in B. longum. The identified proteins represent 21.4% of the predicted 1727 ORFs in the genome and correspond to 30% of the predicted proteome. Moreover 95 hypothetical proteins were experimentally identified. This is the first compilation of a proteomic reference map for the important probiotic organism B. longum NCC2705. The study aimed to define a number of cellular pathways related to important physiological processes at the proteomic level. Proteomic comparison of glucose- and fructose-grown cells revealed that fructose and glucose are catabolized via the same degradation pathway. Interestingly the sugar-binding protein specific to fructose (BL0033) and Frk showed higher levels of expression in cells grown on fructose than on glucose as determined by semiquantitative RT-PCR. BL0033 time course and concentration experiments showed that the induction time and fructose concentration correlates to increased expression of BL0033. At the same time, an ABC (ATP-binding cassette) transporter ATP-binding protein (BL0034) was slightly up-regulated in cells grown on fructose compared with glucose. All of the above results suggest that the uptake of fructose into the cell may be conducted by a specific transport system in which BL0033 might play an important role.
language: eng
source:
identifier: ISSN: 1535-9476
fulltext: fulltext
issn:
  • 15359476
  • 1535-9476
url: Link


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titleA proteome reference map and proteomic analysis of Bifidobacterium longum NCC2705.
creatorYuan, Jing ; Zhu, Li ; Liu, Xiankai ; Li, Ting ; Zhang, Ying ; Ying, Tianyi ; Wang, Bin ; Wang, Junjun ; Dong, Hua ; Feng, Erling ; Li, Qiang ; Wang, Jie ; Wang, Hongxia ; Wei, Kaihua ; Zhang, Xuemin ; Huang, Cuifeng ; Huang, Peitang ; Huang, Liuyu ; Zeng, Ming ; Wang, Hengliang
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identifierISSN: 1535-9476
subjectATP-Binding Cassette Transporters–Metabolism ; Amino Acids–Analysis ; Bacterial Proteins–Chemistry ; Bifidobacterium–Genetics ; Electrophoresis, Gel, Two-Dimensional–Metabolism ; Energy Metabolism–Metabolism ; Fructose–Metabolism ; Glucose–Analysis ; Glycolysis–Metabolism ; Heat-Shock Proteins–Analysis ; Nucleotides–Analysis ; Open Reading Frames–Analysis ; Proteome–Analysis ; Proteomics–Analysis ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization–Analysis ; ATP-Binding Cassette Transporters ; Amino Acids ; Bacterial Proteins ; Heat-Shock Proteins ; Nucleotides ; Proteome ; Fructose ; Glucose
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descriptionA comprehensive proteomic study was carried out to identify and characterize proteins expressed by Bifidobacterium longum NCC2705. A total of 708 spots representing 369 protein entries were identified by MALDI-TOF-MS and/or ESI-MS/MS. Isoelectric point values estimated by gel electrophoresis matched closely with their predicted ones, although some discrepancies exist suggesting that post-translational protein modifications might be common in B. longum. The identified proteins represent 21.4% of the predicted 1727 ORFs in the genome and correspond to 30% of the predicted proteome. Moreover 95 hypothetical proteins were experimentally identified. This is the first compilation of a proteomic reference map for the important probiotic organism B. longum NCC2705. The study aimed to define a number of cellular pathways related to important physiological processes at the proteomic level. Proteomic comparison of glucose- and fructose-grown cells revealed that fructose and glucose are catabolized via the same degradation pathway. Interestingly the sugar-binding protein specific to fructose (BL0033) and Frk showed higher levels of expression in cells grown on fructose than on glucose as determined by semiquantitative RT-PCR. BL0033 time course and concentration experiments showed that the induction time and fructose concentration correlates to increased expression of BL0033. At the same time, an ABC (ATP-binding cassette) transporter ATP-binding protein (BL0034) was slightly up-regulated in cells grown on fructose compared with glucose. All of the above results suggest that the uptake of fructose into the cell may be conducted by a specific transport system in which BL0033 might play an important role.
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