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Serratia marcescens serralysin induces inflammatory responses through protease-activated receptor 2.

The Serratia marcescens-derived protease serralysin is considered to play an important role in the pathogenesis of infection. Protease-activated receptor 2 (PAR-2) is activated by trypsin and also several other trypsin-like serine proteases, leading to the modulation of inflammatory and immune respo... Full description

Journal Title: Infection and immunity January 2007, Vol.75(1), pp.164-174
Main Author: Kida, Yutaka
Other Authors: Inoue, Hiroyoshi , Shimizu, Takashi , Kuwano, Koichi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0019-9567
Link: http://search.proquest.com/docview/68380721/?pq-origsite=primo
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title: Serratia marcescens serralysin induces inflammatory responses through protease-activated receptor 2.
format: Article
creator:
  • Kida, Yutaka
  • Inoue, Hiroyoshi
  • Shimizu, Takashi
  • Kuwano, Koichi
subjects:
  • Ccaat-Enhancer-Binding Proteins–Metabolism
  • Cell Line, Tumor–Immunology
  • Electrophoretic Mobility Shift Assay–Microbiology
  • Hela Cells–Biosynthesis
  • Humans–Biosynthesis
  • Inflammation–Immunology
  • Interleukin-6–Metabolism
  • Interleukin-8–Metabolism
  • Metalloendopeptidases–Analysis
  • Nf-Kappa B–Immunology
  • Promoter Regions, Genetic–Metabolism
  • RNA, Messenger–Enzymology
  • Receptor, Par-2–Immunology
  • Reverse Transcriptase Polymerase Chain Reaction–Metabolism
  • Serratia Marcescens–Metabolism
  • Transcription Factor AP-1–Metabolism
  • Transcriptional Activation–Metabolism
  • Transfection–Metabolism
  • Ccaat-Enhancer-Binding Proteins
  • Interleukin-6
  • Interleukin-8
  • Nf-Kappa B
  • RNA, Messenger
  • Receptor, Par-2
  • Transcription Factor AP-1
  • Metalloendopeptidases
  • Serralysin
ispartof: Infection and immunity, January 2007, Vol.75(1), pp.164-174
description: The Serratia marcescens-derived protease serralysin is considered to play an important role in the pathogenesis of infection. Protease-activated receptor 2 (PAR-2) is activated by trypsin and also several other trypsin-like serine proteases, leading to the modulation of inflammatory and immune responses. However, little is known about the activation of PAR-2 by bacterial proteases and its roles in bacterial infection. In this study, we investigated whether S. marcescens serralysin activates host inflammatory responses through PAR-2. Our results demonstrated that serralysin induces interleukin-6 (IL-6) and IL-8 mRNA expression in a human lung squamous cell carcinoma, EBC-l cells. In addition, serralysin activated activator protein 1 (AP-1)-, CCAAT/enhancer-binding protein (C/EBP)-, and nuclear factor- Kappa B (NF- Kappa B)-driven promoters in EBC-1 cells. An electrophoretic mobility shift assay showed that serralysin activates the binding of AP-1, C/EBP{szligbeta}, and NF- Kappa B in the cells. Inactivation of serralysin resulted in the failure of transactivation of AP-1-, C/EBP-, and NF- Kappa B-driven promoters in the cells. Furthermore, serralysin activated AP-1-, C/EBP-, and NF- Kappa B-driven promoters via PAR-2 in HeLa cells. PAR-2 antagonist peptides decreased serralysin-induced transactivation of AP-1-, C/EBP-, and NF- Kappa B-driven promoters in EBC-1 cells. Considered together, these results suggest that serralysin requires PAR-2 to activate the critical transcription factors AP-1, C/EBP{szligbeta}, and NF- Kappa B for host inflammatory responses.
language: eng
source:
identifier: ISSN: 0019-9567
fulltext: fulltext
issn:
  • 00199567
  • 0019-9567
url: Link


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titleSerratia marcescens serralysin induces inflammatory responses through protease-activated receptor 2.
creatorKida, Yutaka ; Inoue, Hiroyoshi ; Shimizu, Takashi ; Kuwano, Koichi
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identifierISSN: 0019-9567
subjectCcaat-Enhancer-Binding Proteins–Metabolism ; Cell Line, Tumor–Immunology ; Electrophoretic Mobility Shift Assay–Microbiology ; Hela Cells–Biosynthesis ; Humans–Biosynthesis ; Inflammation–Immunology ; Interleukin-6–Metabolism ; Interleukin-8–Metabolism ; Metalloendopeptidases–Analysis ; Nf-Kappa B–Immunology ; Promoter Regions, Genetic–Metabolism ; RNA, Messenger–Enzymology ; Receptor, Par-2–Immunology ; Reverse Transcriptase Polymerase Chain Reaction–Metabolism ; Serratia Marcescens–Metabolism ; Transcription Factor AP-1–Metabolism ; Transcriptional Activation–Metabolism ; Transfection–Metabolism ; Ccaat-Enhancer-Binding Proteins ; Interleukin-6 ; Interleukin-8 ; Nf-Kappa B ; RNA, Messenger ; Receptor, Par-2 ; Transcription Factor AP-1 ; Metalloendopeptidases ; Serralysin
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descriptionThe Serratia marcescens-derived protease serralysin is considered to play an important role in the pathogenesis of infection. Protease-activated receptor 2 (PAR-2) is activated by trypsin and also several other trypsin-like serine proteases, leading to the modulation of inflammatory and immune responses. However, little is known about the activation of PAR-2 by bacterial proteases and its roles in bacterial infection. In this study, we investigated whether S. marcescens serralysin activates host inflammatory responses through PAR-2. Our results demonstrated that serralysin induces interleukin-6 (IL-6) and IL-8 mRNA expression in a human lung squamous cell carcinoma, EBC-l cells. In addition, serralysin activated activator protein 1 (AP-1)-, CCAAT/enhancer-binding protein (C/EBP)-, and nuclear factor- Kappa B (NF- Kappa B)-driven promoters in EBC-1 cells. An electrophoretic mobility shift assay showed that serralysin activates the binding of AP-1, C/EBP{szligbeta}, and NF- Kappa B in the cells. Inactivation of serralysin resulted in the failure of transactivation of AP-1-, C/EBP-, and NF- Kappa B-driven promoters in the cells. Furthermore, serralysin activated AP-1-, C/EBP-, and NF- Kappa B-driven promoters via PAR-2 in HeLa cells. PAR-2 antagonist peptides decreased serralysin-induced transactivation of AP-1-, C/EBP-, and NF- Kappa B-driven promoters in EBC-1 cells. Considered together, these results suggest that serralysin requires PAR-2 to activate the critical transcription factors AP-1, C/EBP{szligbeta}, and NF- Kappa B for host inflammatory responses.
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