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A triacylated lipoprotein from Mycoplasma genitalium activates NF-kappaB through Toll-like receptor 1 (TLR1) and TLR2.

Mycoplasma genitalium is a sexually transmitted bacterial pathogen that causes nongonococcal chlamydia-negative urethritis, mucopurulent cervicitis, endometritis, pelvic inflammatory disease, and tubal factor infertility in humans. However, pathogenic agents that induce inflammatory responses have n... Full description

Journal Title: Infection and immunity August 2008, Vol.76(8), pp.3672-3678
Main Author: Shimizu, Takashi
Other Authors: Kida, Yutaka , Kuwano, Koichi
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1098-5522 ; DOI: 10.1128/IAI.00257-08
Link: http://search.proquest.com/docview/69327129/?pq-origsite=primo
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title: A triacylated lipoprotein from Mycoplasma genitalium activates NF-kappaB through Toll-like receptor 1 (TLR1) and TLR2.
format: Article
creator:
  • Shimizu, Takashi
  • Kida, Yutaka
  • Kuwano, Koichi
subjects:
  • Amino Acid Sequence–Immunology
  • Cell Line–Isolation & Purification
  • Humans–Immunology
  • Lipoproteins–Metabolism
  • Molecular Sequence Data–Chemical Synthesis
  • Mycoplasma Genitalium–Immunology
  • Nf-Kappa B–Immunology
  • Peptides–Immunology
  • Toll-Like Receptor 1–Immunology
  • Toll-Like Receptor 2–Immunology
  • Lipoproteins
  • Nf-Kappa B
  • Peptides
  • Toll-Like Receptor 1
  • Toll-Like Receptor 2
ispartof: Infection and immunity, August 2008, Vol.76(8), pp.3672-3678
description: Mycoplasma genitalium is a sexually transmitted bacterial pathogen that causes nongonococcal chlamydia-negative urethritis, mucopurulent cervicitis, endometritis, pelvic inflammatory disease, and tubal factor infertility in humans. However, pathogenic agents that induce inflammatory responses have not been identified in M. genitalium. In this study, we examined the involvement of Toll-like receptors (TLRs) in activation of the immune response by a lipoprotein from M. genitalium and their active component responsible for NF-kappaB activation. The Triton X-114 detergent phase of M. genitalium was found to induce NF-kappaB through TLR2. The active component of the Triton X-114 detergent phase was a lipoprotein precursor, MG149. The activation of NF-kappaB by MG149 was inhibited by a dominant negative (DN) construct of TLR1 but not by a DN construct of TLR6. These results indicate that the activation of NF-kappaB by MG149 is dependent on TLR1 and TLR2. A synthetic lipopeptide derived from MG149 containing three acyl chains also induced NF-kappaB through TLR1 and TLR2. Thus, the results show that MG149, a triacylated lipoprotein from M. genitalium, activates NF-kappaB through TLR1 and TLR2.
language: eng
source:
identifier: E-ISSN: 1098-5522 ; DOI: 10.1128/IAI.00257-08
fulltext: fulltext
issn:
  • 10985522
  • 1098-5522
url: Link


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titleA triacylated lipoprotein from Mycoplasma genitalium activates NF-kappaB through Toll-like receptor 1 (TLR1) and TLR2.
creatorShimizu, Takashi ; Kida, Yutaka ; Kuwano, Koichi
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identifierE-ISSN: 1098-5522 ; DOI: 10.1128/IAI.00257-08
subjectAmino Acid Sequence–Immunology ; Cell Line–Isolation & Purification ; Humans–Immunology ; Lipoproteins–Metabolism ; Molecular Sequence Data–Chemical Synthesis ; Mycoplasma Genitalium–Immunology ; Nf-Kappa B–Immunology ; Peptides–Immunology ; Toll-Like Receptor 1–Immunology ; Toll-Like Receptor 2–Immunology ; Lipoproteins ; Nf-Kappa B ; Peptides ; Toll-Like Receptor 1 ; Toll-Like Receptor 2
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descriptionMycoplasma genitalium is a sexually transmitted bacterial pathogen that causes nongonococcal chlamydia-negative urethritis, mucopurulent cervicitis, endometritis, pelvic inflammatory disease, and tubal factor infertility in humans. However, pathogenic agents that induce inflammatory responses have not been identified in M. genitalium. In this study, we examined the involvement of Toll-like receptors (TLRs) in activation of the immune response by a lipoprotein from M. genitalium and their active component responsible for NF-kappaB activation. The Triton X-114 detergent phase of M. genitalium was found to induce NF-kappaB through TLR2. The active component of the Triton X-114 detergent phase was a lipoprotein precursor, MG149. The activation of NF-kappaB by MG149 was inhibited by a dominant negative (DN) construct of TLR1 but not by a DN construct of TLR6. These results indicate that the activation of NF-kappaB by MG149 is dependent on TLR1 and TLR2. A synthetic lipopeptide derived from MG149 containing three acyl chains also induced NF-kappaB through TLR1 and TLR2. Thus, the results show that MG149, a triacylated lipoprotein from M. genitalium, activates NF-kappaB through TLR1 and TLR2.
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