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Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients.

Morphine significantly stimulated invertebrate immunocyte intracellular calcium level increases in a concentration-dependent manner in cells preloaded with Fura super(TM) 2/AM. Morphine's action was blocked by prior exposure of the cells to the opiate receptor antagonist naloxone. Various opioid pep... Full description

Journal Title: Comparative biochemistry and physiology. Part B Biochemistry & molecular biology, July 1999, Vol.123(3), pp.295-299
Main Author: Nieto-Fernandez, F E
Other Authors: Mattocks, D , Cavani, F , Salzet, M , Stefano, G B
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1096-4959
Link: http://search.proquest.com/docview/70032604/?pq-origsite=primo
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title: Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients.
format: Article
creator:
  • Nieto-Fernandez, F E
  • Mattocks, D
  • Cavani, F
  • Salzet, M
  • Stefano, G B
subjects:
  • Animals–Metabolism
  • Bivalvia–Pharmacology
  • Calcium–Drug Effects
  • Cells, Cultured–Metabolism
  • Enkephalin, Ala(2)-Mephe(4)-Gly(5)-–Pharmacology
  • Enkephalins–Pharmacology
  • Fura-2–Pharmacology
  • Hemocytes–Metabolism
  • Kinetics–Metabolism
  • Morphine–Metabolism
  • Naloxone–Metabolism
  • Narcotic Antagonists–Metabolism
  • Nitric Oxide–Metabolism
  • Nitric Oxide Synthase–Metabolism
  • Signal Transduction–Metabolism
  • Enkephalins
  • Narcotic Antagonists
  • Enkephalin, Ala(2)-Mephe(4)-Gly(5)-
  • Nitric Oxide
  • Naloxone
  • Morphine
  • Nitric Oxide Synthase
  • Calcium
  • Fura-2
ispartof: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, July 1999, Vol.123(3), pp.295-299
description: Morphine significantly stimulated invertebrate immunocyte intracellular calcium level increases in a concentration-dependent manner in cells preloaded with Fura super(TM) 2/AM. Morphine's action was blocked by prior exposure of the cells to the opiate receptor antagonist naloxone. Various opioid peptides did not exhibit this ability, indicating a morphine- mu sub(3) mediated process. In comparing the sequence of events concerning morphine's action in stimulating both [Ca super(2+)] sub(i) and NO production in these cells, we found that the first event precedes the second by 42 plus or minus 7 s. The opiate stimulation of [Ca super(2+)] sub(i) was attenuated in cells leached of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity in invertebrate immunocytes.
language: eng
source:
identifier: ISSN: 1096-4959
fulltext: fulltext
issn:
  • 10964959
  • 1096-4959
url: Link


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titleMorphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients.
creatorNieto-Fernandez, F E ; Mattocks, D ; Cavani, F ; Salzet, M ; Stefano, G B
contributorNieto-Fernandez, F E (correspondence author) ; Nieto-Fernandez, F E (record owner)
ispartofComparative biochemistry and physiology. Part B, Biochemistry & molecular biology, July 1999, Vol.123(3), pp.295-299
identifierISSN: 1096-4959
subjectAnimals–Metabolism ; Bivalvia–Pharmacology ; Calcium–Drug Effects ; Cells, Cultured–Metabolism ; Enkephalin, Ala(2)-Mephe(4)-Gly(5)-–Pharmacology ; Enkephalins–Pharmacology ; Fura-2–Pharmacology ; Hemocytes–Metabolism ; Kinetics–Metabolism ; Morphine–Metabolism ; Naloxone–Metabolism ; Narcotic Antagonists–Metabolism ; Nitric Oxide–Metabolism ; Nitric Oxide Synthase–Metabolism ; Signal Transduction–Metabolism ; Enkephalins ; Narcotic Antagonists ; Enkephalin, Ala(2)-Mephe(4)-Gly(5)- ; Nitric Oxide ; Naloxone ; Morphine ; Nitric Oxide Synthase ; Calcium ; Fura-2
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descriptionMorphine significantly stimulated invertebrate immunocyte intracellular calcium level increases in a concentration-dependent manner in cells preloaded with Fura super(TM) 2/AM. Morphine's action was blocked by prior exposure of the cells to the opiate receptor antagonist naloxone. Various opioid peptides did not exhibit this ability, indicating a morphine- mu sub(3) mediated process. In comparing the sequence of events concerning morphine's action in stimulating both [Ca super(2+)] sub(i) and NO production in these cells, we found that the first event precedes the second by 42 plus or minus 7 s. The opiate stimulation of [Ca super(2+)] sub(i) was attenuated in cells leached of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity in invertebrate immunocytes.
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titleMorphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients.
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