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Aberrant epigenetic changes and gene expression in cloned cattle dying around birth.

BACKGROUNDAberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes... Full description

Journal Title: BMC developmental biology February 11, 2008, Vol.8, p.14
Main Author: Lin, Li
Other Authors: Li, Qiang , Zhang, Lei , Zhao, Dingsheng , Dai, Yunping , Li, Ning
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1471-213X ; DOI: 10.1186/1471-213X-8-14
Link: http://search.proquest.com/docview/70432938/?pq-origsite=primo
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title: Aberrant epigenetic changes and gene expression in cloned cattle dying around birth.
format: Article
creator:
  • Lin, Li
  • Li, Qiang
  • Zhang, Lei
  • Zhao, Dingsheng
  • Dai, Yunping
  • Li, Ning
subjects:
  • Acetylation–Genetics
  • Actins–Metabolism
  • Animals–Genetics
  • Cattle–Genetics
  • Cloning, Organism–Genetics
  • DNA Methylation–Genetics
  • Epigenesis, Genetic–Metabolism
  • Fetal Death–Genetics
  • Gene Expression Regulation, Developmental–Metabolism
  • Histones–Metabolism
  • Insulin-Like Growth Factor II–Metabolism
  • Organ Specificity–Metabolism
  • Parturition–Metabolism
  • Repetitive Sequences, Nucleic Acid–Metabolism
  • Vascular Endothelial Growth Factor A–Metabolism
  • Actins
  • Histones
  • Vascular Endothelial Growth Factor A
  • Insulin-Like Growth Factor II
ispartof: BMC developmental biology, February 11, 2008, Vol.8, p.14
description: BACKGROUNDAberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (beta-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3). RESULTSAberrant DNA methylation, histone H4 acetylation and gene expression were observed in both cloned groups. The ED group showed relatively fewer severe DNA methylation abnormalities (p < 0.05) but more abnormal histone H4 acetylations (p < 0.05) and more abnormal expression (p < 0.05) of the selected genes compared to the LD group. However, our data also suggest no widespread gene expression abnormalities in the organs of the dead clones. CONCLUSIONDeaths of clones may be ascribed to abnormal expression of a very limited number of genes.
language: eng
source:
identifier: E-ISSN: 1471-213X ; DOI: 10.1186/1471-213X-8-14
fulltext: fulltext
issn:
  • 1471213X
  • 1471-213X
url: Link


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titleAberrant epigenetic changes and gene expression in cloned cattle dying around birth.
creatorLin, Li ; Li, Qiang ; Zhang, Lei ; Zhao, Dingsheng ; Dai, Yunping ; Li, Ning
contributorLin, Li (correspondence author) ; Lin, Li (record owner)
ispartofBMC developmental biology, February 11, 2008, Vol.8, p.14
identifierE-ISSN: 1471-213X ; DOI: 10.1186/1471-213X-8-14
subjectAcetylation–Genetics ; Actins–Metabolism ; Animals–Genetics ; Cattle–Genetics ; Cloning, Organism–Genetics ; DNA Methylation–Genetics ; Epigenesis, Genetic–Metabolism ; Fetal Death–Genetics ; Gene Expression Regulation, Developmental–Metabolism ; Histones–Metabolism ; Insulin-Like Growth Factor II–Metabolism ; Organ Specificity–Metabolism ; Parturition–Metabolism ; Repetitive Sequences, Nucleic Acid–Metabolism ; Vascular Endothelial Growth Factor A–Metabolism ; Actins ; Histones ; Vascular Endothelial Growth Factor A ; Insulin-Like Growth Factor II
descriptionBACKGROUNDAberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (beta-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3). RESULTSAberrant DNA methylation, histone H4 acetylation and gene expression were observed in both cloned groups. The ED group showed relatively fewer severe DNA methylation abnormalities (p < 0.05) but more abnormal histone H4 acetylations (p < 0.05) and more abnormal expression (p < 0.05) of the selected genes compared to the LD group. However, our data also suggest no widespread gene expression abnormalities in the organs of the dead clones. CONCLUSIONDeaths of clones may be ascribed to abnormal expression of a very limited number of genes.
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titleAberrant epigenetic changes and gene expression in cloned cattle dying around birth.
descriptionBACKGROUNDAberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (beta-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3). RESULTSAberrant DNA methylation, histone H4 acetylation and gene expression were observed in both cloned groups. The ED group showed relatively fewer severe DNA methylation abnormalities (p < 0.05) but more abnormal histone H4 acetylations (p < 0.05) and more abnormal expression (p < 0.05) of the selected genes compared to the LD group. However, our data also suggest no widespread gene expression abnormalities in the organs of the dead clones. CONCLUSIONDeaths of clones may be ascribed to abnormal expression of a very limited number of genes.
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authorLin, Li ; Li, Qiang ; Zhang, Lei ; Zhao, Dingsheng ; Dai, Yunping ; Li, Ning
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abstractBACKGROUNDAberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (beta-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3). RESULTSAberrant DNA methylation, histone H4 acetylation and gene expression were observed in both cloned groups. The ED group showed relatively fewer severe DNA methylation abnormalities (p < 0.05) but more abnormal histone H4 acetylations (p < 0.05) and more abnormal expression (p < 0.05) of the selected genes compared to the LD group. However, our data also suggest no widespread gene expression abnormalities in the organs of the dead clones. CONCLUSIONDeaths of clones may be ascribed to abnormal expression of a very limited number of genes.
doi10.1186/1471-213X-8-14
urlhttp://search.proquest.com/docview/70432938/
date2008-02-11