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Genomic organization and characterization of promoter function of the human CDK9 gene.

CDK9 is the catalytic subunit of a general RNA polymerase II (RNAP II) elongation factor termed p-TEFb which is targeted by the human immunodeficiency virus (HIV) Tat protein to activate elongation of the integrated proviral genome. CDK9 mRNA and protein levels have been observed to be induced in ac... Full description

Journal Title: Gene July 11, 2000, Vol.252(1-2), pp.51-59
Main Author: Liu, H
Other Authors: Rice, A P
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: ISSN: 0378-1119
Link: http://search.proquest.com/docview/71230146/?pq-origsite=primo
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title: Genomic organization and characterization of promoter function of the human CDK9 gene.
format: Article
creator:
  • Liu, H
  • Rice, A P
subjects:
  • Base Sequence–Genetics
  • Cyclin-Dependent Kinase 9–Chemistry
  • Cyclin-Dependent Kinases–Genetics
  • DNA–Genetics
  • Exons–Genetics
  • Genes–Metabolism
  • HL-60 Cells–Genetics
  • Hela Cells–Genetics
  • Humans–Metabolism
  • Introns–Metabolism
  • Jurkat Cells–Metabolism
  • Luciferases–Metabolism
  • Molecular Sequence Data–Metabolism
  • Promoter Regions, Genetic–Metabolism
  • Recombinant Fusion Proteins–Metabolism
  • Repetitive Sequences, Nucleic Acid–Metabolism
  • Sequence Analysis, DNA–Metabolism
  • Transcription, Genetic–Metabolism
  • AIDS/HIV
  • Recombinant Fusion Proteins
  • DNA
  • Luciferases
  • Cdk9 Protein, Human
  • Cyclin-Dependent Kinase 9
  • Cyclin-Dependent Kinases
ispartof: Gene, July 11, 2000, Vol.252(1-2), pp.51-59
description: CDK9 is the catalytic subunit of a general RNA polymerase II (RNAP II) elongation factor termed p-TEFb which is targeted by the human immunodeficiency virus (HIV) Tat protein to activate elongation of the integrated proviral genome. CDK9 mRNA and protein levels have been observed to be induced in activated peripheral blood lymphocytes, a cell type relevant to HIV infection. To investigate mechanisms that regulate CDK9 RNA expression, we isolated genomic sequences containing the human CDK9 gene and found that CDK9 coding sequences are interrupted by six introns. There is a major transcriptional start site located 79 nucleotides upstream of the ATG initiator codon at nucleotide +1. Nucleotides −352 to −1 contain all the transcriptional regulatory elements needed for full promoter activity in transient expression assays. The CDK9 promoter contains features characteristic of a housekeeping gene, including GC-rich sequences and absence of a functional TATA element. The CDK9 promoter possesses high constitutive activity and may therefore have utility in expression vectors or gene therapy vectors.
language: eng
source:
identifier: ISSN: 0378-1119
fulltext: fulltext
issn:
  • 03781119
  • 0378-1119
url: Link


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titleGenomic organization and characterization of promoter function of the human CDK9 gene.
creatorLiu, H ; Rice, A P
contributorLiu, H (correspondence author) ; Liu, H (record owner)
ispartofGene, July 11, 2000, Vol.252(1-2), pp.51-59
identifierISSN: 0378-1119
subjectBase Sequence–Genetics ; Cyclin-Dependent Kinase 9–Chemistry ; Cyclin-Dependent Kinases–Genetics ; DNA–Genetics ; Exons–Genetics ; Genes–Metabolism ; HL-60 Cells–Genetics ; Hela Cells–Genetics ; Humans–Metabolism ; Introns–Metabolism ; Jurkat Cells–Metabolism ; Luciferases–Metabolism ; Molecular Sequence Data–Metabolism ; Promoter Regions, Genetic–Metabolism ; Recombinant Fusion Proteins–Metabolism ; Repetitive Sequences, Nucleic Acid–Metabolism ; Sequence Analysis, DNA–Metabolism ; Transcription, Genetic–Metabolism ; AIDS/HIV ; Recombinant Fusion Proteins ; DNA ; Luciferases ; Cdk9 Protein, Human ; Cyclin-Dependent Kinase 9 ; Cyclin-Dependent Kinases
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descriptionCDK9 is the catalytic subunit of a general RNA polymerase II (RNAP II) elongation factor termed p-TEFb which is targeted by the human immunodeficiency virus (HIV) Tat protein to activate elongation of the integrated proviral genome. CDK9 mRNA and protein levels have been observed to be induced in activated peripheral blood lymphocytes, a cell type relevant to HIV infection. To investigate mechanisms that regulate CDK9 RNA expression, we isolated genomic sequences containing the human CDK9 gene and found that CDK9 coding sequences are interrupted by six introns. There is a major transcriptional start site located 79 nucleotides upstream of the ATG initiator codon at nucleotide +1. Nucleotides −352 to −1 contain all the transcriptional regulatory elements needed for full promoter activity in transient expression assays. The CDK9 promoter contains features characteristic of a housekeeping gene, including GC-rich sequences and absence of a functional TATA element. The CDK9 promoter possesses high constitutive activity and may therefore have utility in expression vectors or gene therapy vectors.
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