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Isolation and characterization of the human cyclin T1 promoter.

Cyclin T1 (CycT1) is a regulatory subunit of a general RNA polymerase II (RNAP II) elongation factor termed P-TEFb. The human immunodeficiency virus Tat protein directly associates with CycT1 to utilize CycT1/P-TEFb (also called TAK) for activation of RNAP II elongation of the integrated proviral ge... Full description

Journal Title: Gene July 11, 2000, Vol.252(1-2), pp.39-49
Main Author: Liu, H
Other Authors: Rice, A P
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: ISSN: 0378-1119
Link: http://search.proquest.com/docview/71231103/?pq-origsite=primo
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recordid: proquest71231103
title: Isolation and characterization of the human cyclin T1 promoter.
format: Article
creator:
  • Liu, H
  • Rice, A P
subjects:
  • Base Sequence–Genetics
  • Binding Sites–Chemistry
  • Cyclin T–Isolation & Purification
  • Cyclins–Metabolism
  • DNA–Genetics
  • HL-60 Cells–Metabolism
  • Hela Cells–Genetics
  • Humans–Genetics
  • Jurkat Cells–Metabolism
  • Luciferases–Metabolism
  • Molecular Sequence Data–Metabolism
  • Promoter Regions, Genetic–Metabolism
  • Recombinant Fusion Proteins–Metabolism
  • Sequence Analysis, DNA–Metabolism
  • Transcription Factors–Metabolism
  • Transcription, Genetic–Metabolism
  • AIDS/HIV
  • Ccnt1 Protein, Human
  • Cyclin T
  • Cyclins
  • Recombinant Fusion Proteins
  • Transcription Factors
  • DNA
  • Luciferases
ispartof: Gene, July 11, 2000, Vol.252(1-2), pp.39-49
description: Cyclin T1 (CycT1) is a regulatory subunit of a general RNA polymerase II (RNAP II) elongation factor termed P-TEFb. The human immunodeficiency virus Tat protein directly associates with CycT1 to utilize CycT1/P-TEFb (also called TAK) for activation of RNAP II elongation of the integrated proviral genome. CycT1 mRNA and protein levels are induced in activated human peripheral blood lymphocytes and CycT1 protein levels are induced by a post-transcriptional mechanism when human U937 promonocytic cells are stimulated to differentiate into macrophage-like cells. To investigate mechanisms that regulate CycT1 RNA expression, we isolated the CycT1 promoter. Multiple transcription start sites were identified within 330 nucleotides upstream of the ATG initiation codon at +1. The CycT1 promoter lacks a TATA element and possesses high constitutive activity in plasmid transfection assays. Two distinct regions of the promoter were identified upstream of +1 that contain critical regulatory elements for CycT1 promoter function.
language: eng
source:
identifier: ISSN: 0378-1119
fulltext: fulltext
issn:
  • 03781119
  • 0378-1119
url: Link


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titleIsolation and characterization of the human cyclin T1 promoter.
creatorLiu, H ; Rice, A P
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ispartofGene, July 11, 2000, Vol.252(1-2), pp.39-49
identifierISSN: 0378-1119
subjectBase Sequence–Genetics ; Binding Sites–Chemistry ; Cyclin T–Isolation & Purification ; Cyclins–Metabolism ; DNA–Genetics ; HL-60 Cells–Metabolism ; Hela Cells–Genetics ; Humans–Genetics ; Jurkat Cells–Metabolism ; Luciferases–Metabolism ; Molecular Sequence Data–Metabolism ; Promoter Regions, Genetic–Metabolism ; Recombinant Fusion Proteins–Metabolism ; Sequence Analysis, DNA–Metabolism ; Transcription Factors–Metabolism ; Transcription, Genetic–Metabolism ; AIDS/HIV ; Ccnt1 Protein, Human ; Cyclin T ; Cyclins ; Recombinant Fusion Proteins ; Transcription Factors ; DNA ; Luciferases
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descriptionCyclin T1 (CycT1) is a regulatory subunit of a general RNA polymerase II (RNAP II) elongation factor termed P-TEFb. The human immunodeficiency virus Tat protein directly associates with CycT1 to utilize CycT1/P-TEFb (also called TAK) for activation of RNAP II elongation of the integrated proviral genome. CycT1 mRNA and protein levels are induced in activated human peripheral blood lymphocytes and CycT1 protein levels are induced by a post-transcriptional mechanism when human U937 promonocytic cells are stimulated to differentiate into macrophage-like cells. To investigate mechanisms that regulate CycT1 RNA expression, we isolated the CycT1 promoter. Multiple transcription start sites were identified within 330 nucleotides upstream of the ATG initiation codon at +1. The CycT1 promoter lacks a TATA element and possesses high constitutive activity in plasmid transfection assays. Two distinct regions of the promoter were identified upstream of +1 that contain critical regulatory elements for CycT1 promoter function.
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