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Characterization of the promoter controlling Mona/Gads expression in the megakaryocytic lineage.

Mona/grb2 related adapter downstream of shc is a molecular adapter expressed in platelets, T lymphocytes and myelomonocytic cells. Using human hematopoietic cell lines, we have previously shown that lineage-specific Mona expression is achieved through the production of two transcripts (named 1A and... Full description

Journal Title: Gene August 21, 2002, Vol.296(1-2), pp.151-159
Main Author: Guyot, Boris
Other Authors: Mouchiroud, Guy
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0378-1119
Link: http://search.proquest.com/docview/72184601/?pq-origsite=primo
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title: Characterization of the promoter controlling Mona/Gads expression in the megakaryocytic lineage.
format: Article
creator:
  • Guyot, Boris
  • Mouchiroud, Guy
subjects:
  • Adaptor Proteins, Signal Transducing–Genetics
  • Base Sequence–Genetics
  • Binding Sites–Genetics
  • Carrier Proteins–Metabolism
  • Cell Differentiation–Drug Effects
  • DNA-Binding Proteins–Genetics
  • Electrophoretic Mobility Shift Assay–Metabolism
  • Erythroid-Specific DNA-Binding Factors–Metabolism
  • Gata1 Transcription Factor–Pathology
  • Gene Expression Regulation–Genetics
  • Humans–Metabolism
  • Jurkat Cells–Genetics
  • K562 Cells–Metabolism
  • Luciferases–Genetics
  • Megakaryocytes–Metabolism
  • Molecular Sequence Data–Genetics
  • Promoter Regions, Genetic–Pharmacology
  • Protein Binding–Metabolism
  • Proto-Oncogene Proteins–Genetics
  • Proto-Oncogene Proteins C-Ets
ispartof: Gene, August 21, 2002, Vol.296(1-2), pp.151-159
description: Mona/grb2 related adapter downstream of shc is a molecular adapter expressed in platelets, T lymphocytes and myelomonocytic cells. Using human hematopoietic cell lines, we have previously shown that lineage-specific Mona expression is achieved through the production of two transcripts (named 1A and 1B) differing by their 5′ untranslated region (5′UTR). Thus, platelets and megakaryocytic cell lines K562 and HEL (Human Erythro-Leukemia) specifically express 1B messenger RNA (mRNA). We report here characterization of the (−2031/+72) genomic region relative to the putative transcription start site of 1B mRNA. We show this region is sufficient to ensure specific reporter gene expression in megakaryocytic cell lines, and that most promoter activity is contained in the (−225/+72) fragment. Electro-mobility shift assay and mutational analyses indicated that GATA-1 and a yet unidentified E-26 family member transcription factor are required for 1B (−2031/+72) promoter activity. Thus, Mona 1B promoter exhibits typical features of megakaryocyte-specific promoters.
language: eng
source:
identifier: ISSN: 0378-1119
fulltext: fulltext
issn:
  • 03781119
  • 0378-1119
url: Link


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titleCharacterization of the promoter controlling Mona/Gads expression in the megakaryocytic lineage.
creatorGuyot, Boris ; Mouchiroud, Guy
contributorGuyot, Boris (correspondence author) ; Guyot, Boris (record owner)
ispartofGene, August 21, 2002, Vol.296(1-2), pp.151-159
identifierISSN: 0378-1119
subjectAdaptor Proteins, Signal Transducing–Genetics ; Base Sequence–Genetics ; Binding Sites–Genetics ; Carrier Proteins–Metabolism ; Cell Differentiation–Drug Effects ; DNA-Binding Proteins–Genetics ; Electrophoretic Mobility Shift Assay–Metabolism ; Erythroid-Specific DNA-Binding Factors–Metabolism ; Gata1 Transcription Factor–Pathology ; Gene Expression Regulation–Genetics ; Humans–Metabolism ; Jurkat Cells–Genetics ; K562 Cells–Metabolism ; Luciferases–Genetics ; Megakaryocytes–Metabolism ; Molecular Sequence Data–Genetics ; Promoter Regions, Genetic–Pharmacology ; Protein Binding–Metabolism ; Proto-Oncogene Proteins–Genetics ; Proto-Oncogene Proteins C-Ets
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descriptionMona/grb2 related adapter downstream of shc is a molecular adapter expressed in platelets, T lymphocytes and myelomonocytic cells. Using human hematopoietic cell lines, we have previously shown that lineage-specific Mona expression is achieved through the production of two transcripts (named 1A and 1B) differing by their 5′ untranslated region (5′UTR). Thus, platelets and megakaryocytic cell lines K562 and HEL (Human Erythro-Leukemia) specifically express 1B messenger RNA (mRNA). We report here characterization of the (−2031/+72) genomic region relative to the putative transcription start site of 1B mRNA. We show this region is sufficient to ensure specific reporter gene expression in megakaryocytic cell lines, and that most promoter activity is contained in the (−225/+72) fragment. Electro-mobility shift assay and mutational analyses indicated that GATA-1 and a yet unidentified E-26 family member transcription factor are required for 1B (−2031/+72) promoter activity. Thus, Mona 1B promoter exhibits typical features of megakaryocyte-specific promoters.
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9Gene Expression Regulation–Genetics
10Humans–Metabolism
11Jurkat Cells–Genetics
12K562 Cells–Metabolism
13Luciferases–Genetics
14Megakaryocytes–Metabolism
15Molecular Sequence Data–Genetics
16Promoter Regions, Genetic–Pharmacology
17Protein Binding–Metabolism
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19Proto-Oncogene Proteins C-Ets
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titleCharacterization of the promoter controlling Mona/Gads expression in the megakaryocytic lineage.
authorGuyot, Boris ; Mouchiroud, Guy
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1Base Sequence–Genetics
2Binding Sites–Genetics
3Carrier Proteins–Metabolism
4Cell Differentiation–Drug Effects
5DNA-Binding Proteins–Genetics
6Electrophoretic Mobility Shift Assay–Metabolism
7Erythroid-Specific DNA-Binding Factors–Metabolism
8Gata1 Transcription Factor–Pathology
9Gene Expression Regulation–Genetics
10Humans–Metabolism
11Jurkat Cells–Genetics
12K562 Cells–Metabolism
13Luciferases–Genetics
14Megakaryocytes–Metabolism
15Molecular Sequence Data–Genetics
16Promoter Regions, Genetic–Pharmacology
17Protein Binding–Metabolism
18Proto-Oncogene Proteins–Genetics
19Proto-Oncogene Proteins C-Ets
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date2002-08-21