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RNA polymerase II promoter-proximal pausing upregulates c-fos gene expression.

Transcription elongation regulates c- fos expression in mouse and human cells. In the inactive state of the gene, RNA polymerases are engaged only in the promoter-proximal region. Upon activation, RNA polymerases move efficiently along the complete gene. We have used Epstein–Barr virus (EBV) episome... Full description

Journal Title: Gene September 19, 2000, Vol.255(2), pp.185-194
Main Author: Fivaz, J
Other Authors: Bassi, M C , Pinaud, S , Mirkovitch, J
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
DNA
ID: ISSN: 0378-1119
Link: http://search.proquest.com/docview/72320115/?pq-origsite=primo
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recordid: proquest72320115
title: RNA polymerase II promoter-proximal pausing upregulates c-fos gene expression.
format: Article
creator:
  • Fivaz, J
  • Bassi, M C
  • Pinaud, S
  • Mirkovitch, J
subjects:
  • Animals–Genetics
  • Cell Line–Metabolism
  • DNA–Drug Effects
  • Gene Expression Regulation–Genetics
  • Herpesvirus 4, Human–Genetics
  • Humans–Metabolism
  • Jurkat Cells–Genetics
  • Luciferases–Genetics
  • Mice–Genetics
  • Plasmids–Drug Effects
  • Promoter Regions, Genetic–Genetics
  • Proto-Oncogene Proteins C-Fos–Metabolism
  • RNA–Metabolism
  • RNA Polymerase II–Drug Effects
  • Recombinant Fusion Proteins–Genetics
  • Tetradecanoylphorbol Acetate–Metabolism
  • Transcription, Genetic–Pharmacology
  • Tumor Cells, Cultured–Pharmacology
  • Up-Regulation–Pharmacology
  • Proto-Oncogene Proteins C-Fos
  • Recombinant Fusion Proteins
  • RNA
  • DNA
  • Luciferases
  • RNA Polymerase II
  • Tetradecanoylphorbol Acetate
ispartof: Gene, September 19, 2000, Vol.255(2), pp.185-194
description: Transcription elongation regulates c- fos expression in mouse and human cells. In the inactive state of the gene, RNA polymerases are engaged only in the promoter-proximal region. Upon activation, RNA polymerases move efficiently along the complete gene. We have used Epstein–Barr virus (EBV) episomes as a gene transfer system to study the role of promoter-proximal pausing and transcript elongation in c- fos expression. We find that the sequence located immediately downstream of the transcriptional start site specifies pausing of RNA polymerases, dependent on both its orientation and position relative to the promoter. This sequence is, however, not necessary to maintain repression in the absence of a stimulus. As promoter-proximal pausing is therefore not a repression mechanism for the c- fos gene, the promoter and enhancer sequences are the main determinants of RNA polymerase elongation competence. Surprisingly, we find that promoter-proximal pausing further increases transcriptional levels from a variety of promoters. These observations lead us to hypothesize that promoter-proximal pausing of RNA polymerase II augments c- fos expression by allowing more efficient phosphorylation of the C-terminal domain of the large subunit.
language: eng
source:
identifier: ISSN: 0378-1119
fulltext: fulltext
issn:
  • 03781119
  • 0378-1119
url: Link


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titleRNA polymerase II promoter-proximal pausing upregulates c-fos gene expression.
creatorFivaz, J ; Bassi, M C ; Pinaud, S ; Mirkovitch, J
contributorFivaz, J (correspondence author) ; Fivaz, J (record owner)
ispartofGene, September 19, 2000, Vol.255(2), pp.185-194
identifierISSN: 0378-1119
subjectAnimals–Genetics ; Cell Line–Metabolism ; DNA–Drug Effects ; Gene Expression Regulation–Genetics ; Herpesvirus 4, Human–Genetics ; Humans–Metabolism ; Jurkat Cells–Genetics ; Luciferases–Genetics ; Mice–Genetics ; Plasmids–Drug Effects ; Promoter Regions, Genetic–Genetics ; Proto-Oncogene Proteins C-Fos–Metabolism ; RNA–Metabolism ; RNA Polymerase II–Drug Effects ; Recombinant Fusion Proteins–Genetics ; Tetradecanoylphorbol Acetate–Metabolism ; Transcription, Genetic–Pharmacology ; Tumor Cells, Cultured–Pharmacology ; Up-Regulation–Pharmacology ; Proto-Oncogene Proteins C-Fos ; Recombinant Fusion Proteins ; RNA ; DNA ; Luciferases ; RNA Polymerase II ; Tetradecanoylphorbol Acetate
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descriptionTranscription elongation regulates c- fos expression in mouse and human cells. In the inactive state of the gene, RNA polymerases are engaged only in the promoter-proximal region. Upon activation, RNA polymerases move efficiently along the complete gene. We have used Epstein–Barr virus (EBV) episomes as a gene transfer system to study the role of promoter-proximal pausing and transcript elongation in c- fos expression. We find that the sequence located immediately downstream of the transcriptional start site specifies pausing of RNA polymerases, dependent on both its orientation and position relative to the promoter. This sequence is, however, not necessary to maintain repression in the absence of a stimulus. As promoter-proximal pausing is therefore not a repression mechanism for the c- fos gene, the promoter and enhancer sequences are the main determinants of RNA polymerase elongation competence. Surprisingly, we find that promoter-proximal pausing further increases transcriptional levels from a variety of promoters. These observations lead us to hypothesize that promoter-proximal pausing of RNA polymerase II augments c- fos expression by allowing more efficient phosphorylation of the C-terminal domain of the large subunit.
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authorFivaz, J ; Bassi, M C ; Pinaud, S ; Mirkovitch, J
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