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Precisely positioned nucleosomes are not essential for c-fos gene regulation in vivo.

Chromatin architecture plays a decisive role in many aspects of transcription regulation. We have tested the role of specific chromatin structures in c- fos gene regulation, using a gene transfer system based on episomes derived from the Epstein–Barr virus (EBV). This system reproduces in several re... Full description

Journal Title: Gene September 19, 2000, Vol.255(2), pp.169-184
Main Author: Fivaz, J
Other Authors: Bassi, M C , Price, M , Pinaud, S , Mirkovitch, J
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: ISSN: 0378-1119
Link: http://search.proquest.com/docview/72327452/?pq-origsite=primo
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title: Precisely positioned nucleosomes are not essential for c-fos gene regulation in vivo.
format: Article
creator:
  • Fivaz, J
  • Bassi, M C
  • Price, M
  • Pinaud, S
  • Mirkovitch, J
subjects:
  • Animals–Genetics
  • Cell Line–Pharmacology
  • Chromatin–Genetics
  • Colforsin–Metabolism
  • DNA–Drug Effects
  • Dose-Response Relationship, Drug–Genetics
  • Gene Expression Regulation–Pharmacology
  • Herpesvirus 4, Human–Genetics
  • Humans–Metabolism
  • Ionomycin–Genetics
  • Jurkat Cells–Genetics
  • Luciferases–Genetics
  • Mice–Genetics
  • Nucleosomes–Metabolism
  • Plasmids–Drug Effects
  • Promoter Regions, Genetic–Genetics
  • Proto-Oncogene Proteins C-Fos–Metabolism
  • RNA Polymerase II–Pharmacology
  • Recombinant Fusion Proteins–Pharmacology
  • Tetradecanoylphorbol Acetate–Pharmacology
  • Tumor Cells, Cultured–Pharmacology
  • Chromatin
  • Nucleosomes
  • Proto-Oncogene Proteins C-Fos
  • Recombinant Fusion Proteins
  • Colforsin
  • Ionomycin
  • DNA
  • Luciferases
  • RNA Polymerase II
  • Tetradecanoylphorbol Acetate
ispartof: Gene, September 19, 2000, Vol.255(2), pp.169-184
description: Chromatin architecture plays a decisive role in many aspects of transcription regulation. We have tested the role of specific chromatin structures in c- fos gene regulation, using a gene transfer system based on episomes derived from the Epstein–Barr virus (EBV). This system reproduces in several respects the chromatin structure and regulation of the chromosomal c- fos gene. Using this approach, we first demonstrate that the pausing of RNA polymerase II downstream of the transcriptional start site does not require precisely positioned nucleosomes. Indeed, changing the pattern of MNase hypersensitive sites along the transcribed sequence does not perturb RNA polymerase II pausing or the regulation of the c- fos gene. Next, we show that a putative nucleosome positioned between the SIE/SRE elements (−300) and the CRE/TATA elements (−36) is not necessary for activation by a variety of inducers. Accordingly, total or partial deletion of the putative nucleosome sequence does not disturb c- fos regulation while the two regulatory sites flanking the nucleosome sequence remain hypersensitive to MNase. As described in this paper, EBV episomes are useful vectors to critically examine the role of the chromatin structure in gene transcription for human cells.
language: eng
source:
identifier: ISSN: 0378-1119
fulltext: fulltext
issn:
  • 03781119
  • 0378-1119
url: Link


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titlePrecisely positioned nucleosomes are not essential for c-fos gene regulation in vivo.
creatorFivaz, J ; Bassi, M C ; Price, M ; Pinaud, S ; Mirkovitch, J
contributorFivaz, J (correspondence author) ; Fivaz, J (record owner)
ispartofGene, September 19, 2000, Vol.255(2), pp.169-184
identifierISSN: 0378-1119
subjectAnimals–Genetics ; Cell Line–Pharmacology ; Chromatin–Genetics ; Colforsin–Metabolism ; DNA–Drug Effects ; Dose-Response Relationship, Drug–Genetics ; Gene Expression Regulation–Pharmacology ; Herpesvirus 4, Human–Genetics ; Humans–Metabolism ; Ionomycin–Genetics ; Jurkat Cells–Genetics ; Luciferases–Genetics ; Mice–Genetics ; Nucleosomes–Metabolism ; Plasmids–Drug Effects ; Promoter Regions, Genetic–Genetics ; Proto-Oncogene Proteins C-Fos–Metabolism ; RNA Polymerase II–Pharmacology ; Recombinant Fusion Proteins–Pharmacology ; Tetradecanoylphorbol Acetate–Pharmacology ; Tumor Cells, Cultured–Pharmacology ; Chromatin ; Nucleosomes ; Proto-Oncogene Proteins C-Fos ; Recombinant Fusion Proteins ; Colforsin ; Ionomycin ; DNA ; Luciferases ; RNA Polymerase II ; Tetradecanoylphorbol Acetate
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descriptionChromatin architecture plays a decisive role in many aspects of transcription regulation. We have tested the role of specific chromatin structures in c- fos gene regulation, using a gene transfer system based on episomes derived from the Epstein–Barr virus (EBV). This system reproduces in several respects the chromatin structure and regulation of the chromosomal c- fos gene. Using this approach, we first demonstrate that the pausing of RNA polymerase II downstream of the transcriptional start site does not require precisely positioned nucleosomes. Indeed, changing the pattern of MNase hypersensitive sites along the transcribed sequence does not perturb RNA polymerase II pausing or the regulation of the c- fos gene. Next, we show that a putative nucleosome positioned between the SIE/SRE elements (−300) and the CRE/TATA elements (−36) is not necessary for activation by a variety of inducers. Accordingly, total or partial deletion of the putative nucleosome sequence does not disturb c- fos regulation while the two regulatory sites flanking the nucleosome sequence remain hypersensitive to MNase. As described in this paper, EBV episomes are useful vectors to critically examine the role of the chromatin structure in gene transcription for human cells.
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8Humans–Metabolism
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14Plasmids–Drug Effects
15Promoter Regions, Genetic–Genetics
16Proto-Oncogene Proteins C-Fos–Metabolism
17RNA Polymerase II–Pharmacology
18Recombinant Fusion Proteins–Pharmacology
19Tetradecanoylphorbol Acetate–Pharmacology
20Tumor Cells, Cultured–Pharmacology
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22Nucleosomes
23Proto-Oncogene Proteins C-Fos
24Recombinant Fusion Proteins
25Colforsin
26Ionomycin
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28Luciferases
29RNA Polymerase II
30Tetradecanoylphorbol Acetate
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titlePrecisely positioned nucleosomes are not essential for c-fos gene regulation in vivo.
authorFivaz, J ; Bassi, M C ; Price, M ; Pinaud, S ; Mirkovitch, J
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6Gene Expression Regulation–Pharmacology
7Herpesvirus 4, Human–Genetics
8Humans–Metabolism
9Ionomycin–Genetics
10Jurkat Cells–Genetics
11Luciferases–Genetics
12Mice–Genetics
13Nucleosomes–Metabolism
14Plasmids–Drug Effects
15Promoter Regions, Genetic–Genetics
16Proto-Oncogene Proteins C-Fos–Metabolism
17RNA Polymerase II–Pharmacology
18Recombinant Fusion Proteins–Pharmacology
19Tetradecanoylphorbol Acetate–Pharmacology
20Tumor Cells, Cultured–Pharmacology
21Chromatin
22Nucleosomes
23Proto-Oncogene Proteins C-Fos
24Recombinant Fusion Proteins
25Colforsin
26Ionomycin
27DNA
28Luciferases
29RNA Polymerase II
30Tetradecanoylphorbol Acetate
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