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Microfluidic Western blot.

We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze... Full description

Journal Title: Analytical chemistry May 15, 2010, Vol.82(10), pp.3974-3976
Main Author: Pan, Wenying
Other Authors: Chen, Wei , Jiang, Xingyu
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; DOI: 1520-6882 ; DOI: 10.1021/ac1000493
Link: http://search.proquest.com/docview/733521133/?pq-origsite=primo
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title: Microfluidic Western blot.
format: Article
creator:
  • Pan, Wenying
  • Chen, Wei
  • Jiang, Xingyu
subjects:
  • Antibodies–Analysis
  • Biomarkers–Methods
  • Blotting, Western–Methods
  • Microfluidics–Analysis
  • Proteins–Analysis
  • Antibodies
  • Biomarkers
  • Proteins
ispartof: Analytical chemistry, May 15, 2010, Vol.82(10), pp.3974-3976
description: We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single sample, and it requires only about 1% of the amount of antibody used in conventional Western blot. 10.1021/ac1000493
language: eng
source:
identifier: E-ISSN: 1520-6882 ; DOI: 1520-6882 ; DOI: 10.1021/ac1000493
fulltext: no_fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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descriptionWe develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single sample, and it requires only about 1% of the amount of antibody used in conventional Western blot. 10.1021/ac1000493
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