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Development of a naturally miniaturised testing method based on the mitochondrial activity of fern spores: a new higher plant bioassay.

One of the main concerns of current environmental toxicology is the low number of taxa used for standard bioassays. Ferns, with more than 10000 living species, are the second largest group of vascular plants and are important components of numerous plant communities. Fern spores and gametophytes hav... Full description

Journal Title: Chemosphere November 2009, Vol.77(7), pp.983-988
Main Author: Catala, Myriam
Other Authors: Esteban, Marta , Rodríguez-Gil, José-Luis , Quintanilla, Luis G
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1879-1298 ; DOI: 10.1016/j.chemosphere.2009.07.080
Link: http://search.proquest.com/docview/734090044/?pq-origsite=primo
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title: Development of a naturally miniaturised testing method based on the mitochondrial activity of fern spores: a new higher plant bioassay.
format: Article
creator:
  • Catala, Myriam
  • Esteban, Marta
  • Rodríguez-Gil, José-Luis
  • Quintanilla, Luis G
subjects:
  • Biological Assay–Methods
  • Dryopteris–Metabolism
  • Mitochondria–Drug Effects
  • Oxidation-Reduction–Metabolism
  • Spores–Metabolism
  • Tetrazolium Salts–Chemistry
  • Toxicity Tests–Toxicity
  • Tetrazolium Salts
ispartof: Chemosphere, November 2009, Vol.77(7), pp.983-988
description: One of the main concerns of current environmental toxicology is the low number of taxa used for standard bioassays. Ferns, with more than 10000 living species, are the second largest group of vascular plants and are important components of numerous plant communities. Fern spores and gametophytes have long been recognized as useful models for plant research since they constitute a naturally miniaturised and economic higher plant model. Mitochondria are the main energy source in eukaryotic cells and any toxic damage will affect the whole organism. The reduction of tetrazolium salts to water-insoluble coloured formazan salts by the respiratory chain has been used for more than 50years as a measure of cell mitochondrial activity and viability in eukaryotic organisms. Here, the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by mitochondria is adapted and optimized to measure fern spore or gametophyte viability. Procedures selected as optimum in the model species Dryopteris guanchica are as follows: bleach sterilization, incubation without shaking at 20 super(o)C in the dark for 1-4h with 0.05-1.5% TTC in Dyer medium supplemented with 0.001-0.005% Tween 20 at pH 8. We conclude that this method constitutes a promising low cost bioassay for higher plant toxicity during development.
language: eng
source:
identifier: E-ISSN: 1879-1298 ; DOI: 10.1016/j.chemosphere.2009.07.080
fulltext: fulltext
issn:
  • 18791298
  • 1879-1298
url: Link


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titleDevelopment of a naturally miniaturised testing method based on the mitochondrial activity of fern spores: a new higher plant bioassay.
creatorCatala, Myriam ; Esteban, Marta ; Rodríguez-Gil, José-Luis ; Quintanilla, Luis G
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ispartofChemosphere, November 2009, Vol.77(7), pp.983-988
identifierE-ISSN: 1879-1298 ; DOI: 10.1016/j.chemosphere.2009.07.080
subjectBiological Assay–Methods ; Dryopteris–Metabolism ; Mitochondria–Drug Effects ; Oxidation-Reduction–Metabolism ; Spores–Metabolism ; Tetrazolium Salts–Chemistry ; Toxicity Tests–Toxicity ; Tetrazolium Salts
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descriptionOne of the main concerns of current environmental toxicology is the low number of taxa used for standard bioassays. Ferns, with more than 10000 living species, are the second largest group of vascular plants and are important components of numerous plant communities. Fern spores and gametophytes have long been recognized as useful models for plant research since they constitute a naturally miniaturised and economic higher plant model. Mitochondria are the main energy source in eukaryotic cells and any toxic damage will affect the whole organism. The reduction of tetrazolium salts to water-insoluble coloured formazan salts by the respiratory chain has been used for more than 50years as a measure of cell mitochondrial activity and viability in eukaryotic organisms. Here, the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by mitochondria is adapted and optimized to measure fern spore or gametophyte viability. Procedures selected as optimum in the model species Dryopteris guanchica are as follows: bleach sterilization, incubation without shaking at 20 super(o)C in the dark for 1-4h with 0.05-1.5% TTC in Dyer medium supplemented with 0.001-0.005% Tween 20 at pH 8. We conclude that this method constitutes a promising low cost bioassay for higher plant toxicity during development.
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