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NEBcutter: A program to cleave DNA with restriction enzymes.

NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, the... Full description

Journal Title: Nucleic acids research July 1, 2003, Vol.31(13), pp.3688-3691
Main Author: Vincze, Tamas
Other Authors: Posfai, Janos , Roberts, Richard J
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1362-4962
Link: http://search.proquest.com/docview/73420919/?pq-origsite=primo
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recordid: proquest73420919
title: NEBcutter: A program to cleave DNA with restriction enzymes.
format: Article
creator:
  • Vincze, Tamas
  • Posfai, Janos
  • Roberts, Richard J
subjects:
  • DNA Methylation–Metabolism
  • DNA Restriction Enzymes–Metabolism
  • Deoxyribonucleases, Type II Site-Specific–Methods
  • Internet–Methods
  • Plasmids–Methods
  • Restriction Mapping–Methods
  • Sequence Analysis, DNA–Methods
  • Software–Methods
  • Substrate Specificity–Methods
  • DNA Restriction Enzymes
  • Deoxyribonucleases, Type II Site-Specific
ispartof: Nucleic acids research, July 1, 2003, Vol.31(13), pp.3688-3691
description: NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme database, REBASE (http://www.neb.com/rebase). Importantly, its table of recognition sites is updated daily from REBASE and it marks all sites that are potentially affected by DNA methylation (Dam, Dcm, etc.). Many options exist to choose the enzymes used for digestion, including all known specificities, subsets of those that are commercially available or sets of enzymes that produce compatible termini.
language: eng
source:
identifier: E-ISSN: 1362-4962
fulltext: fulltext
issn:
  • 13624962
  • 1362-4962
url: Link


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subjectDNA Methylation–Metabolism ; DNA Restriction Enzymes–Metabolism ; Deoxyribonucleases, Type II Site-Specific–Methods ; Internet–Methods ; Plasmids–Methods ; Restriction Mapping–Methods ; Sequence Analysis, DNA–Methods ; Software–Methods ; Substrate Specificity–Methods ; DNA Restriction Enzymes ; Deoxyribonucleases, Type II Site-Specific
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descriptionNEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme database, REBASE (http://www.neb.com/rebase). Importantly, its table of recognition sites is updated daily from REBASE and it marks all sites that are potentially affected by DNA methylation (Dam, Dcm, etc.). Many options exist to choose the enzymes used for digestion, including all known specificities, subsets of those that are commercially available or sets of enzymes that produce compatible termini.
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