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A note on poly-L-lysine-mediated gene transfer in HeLa cells.

Poly-L-lysines of chain lengths varying from 70 to 300 residues are shown to bring about luciferase pRSVL DNA uptake and expression in HeLa cells. Transfection was approximately 50% that of the cationic liposome DOTAB. Expression was higher in the presence of chloroquine. Of interest was the fact th... Full description

Journal Title: Drug delivery 2003 Jul-Sep, Vol.10(3), pp.209-211
Main Author: Joubert, Daniel
Other Authors: van Zyl, Johann , Hawtrey, Arthur , Ariatti, Mario
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: ISSN: 1071-7544
Link: http://search.proquest.com/docview/73590495/?pq-origsite=primo
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title: A note on poly-L-lysine-mediated gene transfer in HeLa cells.
format: Article
creator:
  • Joubert, Daniel
  • van Zyl, Johann
  • Hawtrey, Arthur
  • Ariatti, Mario
subjects:
  • DNA–Administration & Dosage
  • Gene Transfer Techniques–Chemistry
  • Hela Cells–Biosynthesis
  • Humans–Genetics
  • Liposomes–Chemistry
  • Luciferases–Chemistry
  • Plasmids–Chemistry
  • Polylysine–Chemistry
  • Liposomes
  • Polylysine
  • DNA
  • Luciferases
ispartof: Drug delivery, 2003 Jul-Sep, Vol.10(3), pp.209-211
description: Poly-L-lysines of chain lengths varying from 70 to 300 residues are shown to bring about luciferase pRSVL DNA uptake and expression in HeLa cells. Transfection was approximately 50% that of the cationic liposome DOTAB. Expression was higher in the presence of chloroquine. Of interest was the fact that luciferase activity depended on the polysine/DNA charge ratio (+/-). Maximum activity occurred at a charge ratio (+/-) of 3, while at a charge ratio of 1 (conjugate electrically neutral) activity was much lower. At the higher charge ratios (+/-) of 4 and 5, luciferase activity decreased. The results obtained are discussed.
language: eng
source:
identifier: ISSN: 1071-7544
fulltext: fulltext
issn:
  • 10717544
  • 1071-7544
url: Link


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titleA note on poly-L-lysine-mediated gene transfer in HeLa cells.
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identifierISSN: 1071-7544
subjectDNA–Administration & Dosage ; Gene Transfer Techniques–Chemistry ; Hela Cells–Biosynthesis ; Humans–Genetics ; Liposomes–Chemistry ; Luciferases–Chemistry ; Plasmids–Chemistry ; Polylysine–Chemistry ; Liposomes ; Polylysine ; DNA ; Luciferases
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descriptionPoly-L-lysines of chain lengths varying from 70 to 300 residues are shown to bring about luciferase pRSVL DNA uptake and expression in HeLa cells. Transfection was approximately 50% that of the cationic liposome DOTAB. Expression was higher in the presence of chloroquine. Of interest was the fact that luciferase activity depended on the polysine/DNA charge ratio (+/-). Maximum activity occurred at a charge ratio (+/-) of 3, while at a charge ratio of 1 (conjugate electrically neutral) activity was much lower. At the higher charge ratios (+/-) of 4 and 5, luciferase activity decreased. The results obtained are discussed.
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titleA note on poly-L-lysine-mediated gene transfer in HeLa cells.
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