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Linking power Doppler ultrasound to the presence of th17 cells in the rheumatoid arthritis joint.

BACKGROUNDPower Doppler ultrasound (PDUS) is increasingly used to assess synovitis in Rheumatoid Arthritis (RA). Prior studies have shown correlations between PDUS scores and vessel counts, but relationships with T cell immunopathology have not been described. METHODOLOGY/PRINCIPAL FINDINGSPBMC were... Full description

Journal Title: PloS one September 1, 2010, Vol.5(9)
Main Author: Gullick, Nicola J
Other Authors: Evans, Hayley G , Church, Leigh D , Jayaraj, David M , Filer, Andrew , Kirkham, Bruce W , Taams, Leonie S
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0012516
Link: http://search.proquest.com/docview/754000280/?pq-origsite=primo
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title: Linking power Doppler ultrasound to the presence of th17 cells in the rheumatoid arthritis joint.
format: Article
creator:
  • Gullick, Nicola J
  • Evans, Hayley G
  • Church, Leigh D
  • Jayaraj, David M
  • Filer, Andrew
  • Kirkham, Bruce W
  • Taams, Leonie S
subjects:
  • Adult–Diagnosis
  • Aged–Diagnostic Imaging
  • Arthritis, Rheumatoid–Immunology
  • Case-Control Studies–Immunology
  • Cells, Cultured–Diagnostic Imaging
  • Cytokines–Immunology
  • Female–Immunology
  • Humans–Diagnostic Imaging
  • Joints–Immunology
  • Male–Immunology
  • Middle Aged–Immunology
  • Synovial Fluid–Immunology
  • Synovitis–Immunology
  • Th17 Cells–Immunology
  • Ultrasonography, Doppler–Immunology
  • Cytokines
ispartof: PloS one, September 1, 2010, Vol.5(9)
description: BACKGROUNDPower Doppler ultrasound (PDUS) is increasingly used to assess synovitis in Rheumatoid Arthritis (RA). Prior studies have shown correlations between PDUS scores and vessel counts, but relationships with T cell immunopathology have not been described. METHODOLOGY/PRINCIPAL FINDINGSPBMC were isolated from healthy controls (HC) or RA patients and stimulated ex vivo with PMA and ionomycin for 3 hours in the presence of Golgistop. Paired synovial fluid (SF) or synovial tissue (ST) were analysed where available. Intracellular expression of IL-17, IFNgamma, and TNFalpha by CD4+ T cells was determined by flow cytometry. Synovial blood flow was evaluated by PDUS signal at the knees, wrists and metacarpophalangeal joints of RA patients. Serum, SF and fibroblast culture supernatant levels of vascular endothelial growth factor-A (VEGF-A) were measured by ELISA. The frequency of IL17+IFNgamma-CD4+ T cells (Th17 cells) was significantly elevated in peripheral blood (PB) from RA patients vs....
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0012516
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleLinking power Doppler ultrasound to the presence of th17 cells in the rheumatoid arthritis joint.
creatorGullick, Nicola J ; Evans, Hayley G ; Church, Leigh D ; Jayaraj, David M ; Filer, Andrew ; Kirkham, Bruce W ; Taams, Leonie S
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0012516
subjectAdult–Diagnosis ; Aged–Diagnostic Imaging ; Arthritis, Rheumatoid–Immunology ; Case-Control Studies–Immunology ; Cells, Cultured–Diagnostic Imaging ; Cytokines–Immunology ; Female–Immunology ; Humans–Diagnostic Imaging ; Joints–Immunology ; Male–Immunology ; Middle Aged–Immunology ; Synovial Fluid–Immunology ; Synovitis–Immunology ; Th17 Cells–Immunology ; Ultrasonography, Doppler–Immunology ; Cytokines
descriptionBACKGROUNDPower Doppler ultrasound (PDUS) is increasingly used to assess synovitis in Rheumatoid Arthritis (RA). Prior studies have shown correlations between PDUS scores and vessel counts, but relationships with T cell immunopathology have not been described. METHODOLOGY/PRINCIPAL FINDINGSPBMC were isolated from healthy controls (HC) or RA patients and stimulated ex vivo with PMA and ionomycin for 3 hours in the presence of Golgistop. Paired synovial fluid (SF) or synovial tissue (ST) were analysed where available. Intracellular expression of IL-17, IFNgamma, and TNFalpha by CD4+ T cells was determined by flow cytometry. Synovial blood flow was evaluated by PDUS signal at the knees, wrists and metacarpophalangeal joints of RA patients. Serum, SF and fibroblast culture supernatant levels of vascular endothelial growth factor-A (VEGF-A) were measured by ELISA. The frequency of IL17+IFNgamma-CD4+ T cells (Th17 cells) was significantly elevated in peripheral blood (PB) from RA patients vs....
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titleLinking power Doppler ultrasound to the presence of th17 cells in the rheumatoid arthritis joint.
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titleLinking power Doppler ultrasound to the presence of th17 cells in the rheumatoid arthritis joint.
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abstractBACKGROUNDPower Doppler ultrasound (PDUS) is increasingly used to assess synovitis in Rheumatoid Arthritis (RA). Prior studies have shown correlations between PDUS scores and vessel counts, but relationships with T cell immunopathology have not been described. METHODOLOGY/PRINCIPAL FINDINGSPBMC were isolated from healthy controls (HC) or RA patients and stimulated ex vivo with PMA and ionomycin for 3 hours in the presence of Golgistop. Paired synovial fluid (SF) or synovial tissue (ST) were analysed where available. Intracellular expression of IL-17, IFNgamma, and TNFalpha by CD4+ T cells was determined by flow cytometry. Synovial blood flow was evaluated by PDUS signal at the knees, wrists and metacarpophalangeal joints of RA patients. Serum, SF and fibroblast culture supernatant levels of vascular endothelial growth factor-A (VEGF-A) were measured by ELISA. The frequency of IL17+IFNgamma-CD4+ T cells (Th17 cells) was significantly elevated in peripheral blood (PB) from RA patients vs....
doi10.1371/journal.pone.0012516
urlhttp://search.proquest.com/docview/754000280/
pages1-11
date2010-09-01