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Selection of reference genes for gene expression studies in astrocytomas.

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ab.2010.09.010 Byline: Sylwia M. Gresner (a), Ewa Golanska (a), Dominika Kulczycka-Wojdala (b), Dariusz J. Jaskolski (c), Wielislaw Papierz (d), Pawel P. Liberski (a) Abstract: This study was aimed to test a p... Full description

Journal Title: Analytical biochemistry January 1, 2011, Vol.408(1), pp.163-165
Main Author: Gresner, Sylwia M
Other Authors: Golanska, Ewa , Kulczycka-Wojdala, Dominika , Jaskolski, Dariusz J , Papierz, Wielislaw , Liberski, Pawel P
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1096-0309 ; DOI: 10.1016/j.ab.2010.09.010
Link: http://search.proquest.com/docview/760152392/?pq-origsite=primo
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title: Selection of reference genes for gene expression studies in astrocytomas.
format: Article
creator:
  • Gresner, Sylwia M
  • Golanska, Ewa
  • Kulczycka-Wojdala, Dominika
  • Jaskolski, Dariusz J
  • Papierz, Wielislaw
  • Liberski, Pawel P
subjects:
  • Actins–Genetics
  • Astrocytoma–Metabolism
  • Gene Expression Profiling–Standards
  • Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)–Genetics
  • Histones–Metabolism
  • Humans–Genetics
  • Hypoxanthine Phosphoribosyltransferase–Metabolism
  • Polymerase Chain Reaction–Standards
  • Reference Standards–Genetics
  • Ribosomal Proteins–Metabolism
  • Ribosomal Proteins–Standards
  • Ribosomal Proteins–Genetics
  • Ribosomal Proteins–Metabolism
  • Ribosomal Proteins–Standards
  • Ribosomal Proteins–Methods
  • Ribosomal Proteins–Standards
  • Ribosomal Proteins–Genetics
  • Ribosomal Proteins–Metabolism
  • Ribosomal Proteins–Standards
  • Actins
ispartof: Analytical biochemistry, January 1, 2011, Vol.408(1), pp.163-165
description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ab.2010.09.010 Byline: Sylwia M. Gresner (a), Ewa Golanska (a), Dominika Kulczycka-Wojdala (b), Dariusz J. Jaskolski (c), Wielislaw Papierz (d), Pawel P. Liberski (a) Abstract: This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene. The effect of reference gene selection on quantitative real-time polymerase chain reaction data interpretation was demonstrated, normalizing the expression data of a selected gene of interest. Thus, GAPDH may be recommended for data normalization in gene expression studies in astrocytomas. Nevertheless, a preliminary validation of reference gene stability is required prior to every study. Author Affiliation: (a) Department of Molecular Pathology and Neuropathology, Medical University of Lodz, 92-216 Lodz, Poland (b) Department of Oncological Pathology, Medical University of Lodz, 93-509 Lodz, Poland (c) Department of Neurosurgery, Medical University of Lodz, 90-153 Lodz, Poland (d) Department of Pathomorphology, Medical University of Lodz, 92-216 Lodz, Poland Article History: Received 18 June 2010; Revised 2 September 2010; Accepted 4 September 2010
language: eng
source:
identifier: E-ISSN: 1096-0309 ; DOI: 10.1016/j.ab.2010.09.010
fulltext: fulltext
issn:
  • 10960309
  • 1096-0309
url: Link


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titleSelection of reference genes for gene expression studies in astrocytomas.
creatorGresner, Sylwia M ; Golanska, Ewa ; Kulczycka-Wojdala, Dominika ; Jaskolski, Dariusz J ; Papierz, Wielislaw ; Liberski, Pawel P
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identifierE-ISSN: 1096-0309 ; DOI: 10.1016/j.ab.2010.09.010
subjectActins–Genetics ; Astrocytoma–Metabolism ; Gene Expression Profiling–Standards ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)–Genetics ; Histones–Metabolism ; Humans–Genetics ; Hypoxanthine Phosphoribosyltransferase–Metabolism ; Polymerase Chain Reaction–Standards ; Reference Standards–Genetics ; Ribosomal Proteins–Metabolism ; Ribosomal Proteins–Standards ; Ribosomal Proteins–Genetics ; Ribosomal Proteins–Metabolism ; Ribosomal Proteins–Standards ; Ribosomal Proteins–Methods ; Ribosomal Proteins–Standards ; Ribosomal Proteins–Genetics ; Ribosomal Proteins–Metabolism ; Ribosomal Proteins–Standards ; Actins
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