schliessen

Filtern

 

Bibliotheken

Acute treatment of mice with cadmium salts results in amplification of the metallothionein-1 gene in liver.

A variety of genes have been shown to change copy number during development, including rRNA genes in amphibians and chorion proteins in insects. Dihydrofolate reductase and metallothionein-1 (MT-1) genes are present in high copy number in cultured mammalian cells subjected to low levels of agents th... Full description

Journal Title: Nucleic acids research August 12, 1985, Vol.13(15), pp.5423-5439
Main Author: Koropatnick, J
Other Authors: Winning, R , Wiese, E , Heschl, M , Gedamu, L , Duerksen, J
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: ISSN: 0305-1048
Link: http://search.proquest.com/docview/76296600/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest76296600
title: Acute treatment of mice with cadmium salts results in amplification of the metallothionein-1 gene in liver.
format: Article
creator:
  • Koropatnick, J
  • Winning, R
  • Wiese, E
  • Heschl, M
  • Gedamu, L
  • Duerksen, J
subjects:
  • Animals–Pharmacology
  • Cadmium–Drug Effects
  • Cadmium Compounds–Metabolism
  • Cell Nucleus–Genetics
  • DNA–Metabolism
  • DNA Replication–Drug Effects
  • Gene Amplification–Drug Effects
  • Kinetics–Ultrastructure
  • Liver–Genetics
  • Male–Pharmacology
  • Metallothionein–Drug Effects
  • Mice–Genetics
  • Micrococcal Nuclease–Genetics
  • Nucleic Acid Hybridization–Genetics
  • Sulfates–Genetics
  • Transcription, Genetic–Genetics
  • Alpha-Fetoproteins–Genetics
  • Cadmium Compounds
  • Sulfates
  • Alpha-Fetoproteins
  • Cadmium
  • DNA
  • Metallothionein
  • Cadmium Sulfate
  • Micrococcal Nuclease
ispartof: Nucleic acids research, August 12, 1985, Vol.13(15), pp.5423-5439
description: A variety of genes have been shown to change copy number during development, including rRNA genes in amphibians and chorion proteins in insects. Dihydrofolate reductase and metallothionein-1 (MT-1) genes are present in high copy number in cultured mammalian cells subjected to low levels of agents that will select for cells with amplified copies of specific genes. Recent studies have shown that the metallothlonein-1 gene in mouse liver is regulated at the transcriptional level by treatment with heavy metals. We report here that, at cadmium concentrations 5 to 10-fold higher than that required to induce maximal transcription of the MT-1 gene, there is a 2 to 3-fold increase in MT-1 gene concentration in liver nuclear DNA by 6 hours after induction, and extra copies persist up to 3 weeks in the absence of further heavy metal treatment. The extra MT-1 gene copies that appear 6 hours after cadmium treatment are in a conformation that renders them relatively nuclease insensitive.
language: eng
source:
identifier: ISSN: 0305-1048
fulltext: fulltext
issn:
  • 03051048
  • 0305-1048
url: Link


@attributes
ID765854370
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid76296600
sourceidproquest
recordidTN_proquest76296600
sourcesystemOther
pqid76296600
display
typearticle
titleAcute treatment of mice with cadmium salts results in amplification of the metallothionein-1 gene in liver.
creatorKoropatnick, J ; Winning, R ; Wiese, E ; Heschl, M ; Gedamu, L ; Duerksen, J
contributorKoropatnick, J (correspondence author) ; Koropatnick, J (record owner)
ispartofNucleic acids research, August 12, 1985, Vol.13(15), pp.5423-5439
identifierISSN: 0305-1048
subjectAnimals–Pharmacology ; Cadmium–Drug Effects ; Cadmium Compounds–Metabolism ; Cell Nucleus–Genetics ; DNA–Metabolism ; DNA Replication–Drug Effects ; Gene Amplification–Drug Effects ; Kinetics–Ultrastructure ; Liver–Genetics ; Male–Pharmacology ; Metallothionein–Drug Effects ; Mice–Genetics ; Micrococcal Nuclease–Genetics ; Nucleic Acid Hybridization–Genetics ; Sulfates–Genetics ; Transcription, Genetic–Genetics ; Alpha-Fetoproteins–Genetics ; Cadmium Compounds ; Sulfates ; Alpha-Fetoproteins ; Cadmium ; DNA ; Metallothionein ; Cadmium Sulfate ; Micrococcal Nuclease
languageeng
source
descriptionA variety of genes have been shown to change copy number during development, including rRNA genes in amphibians and chorion proteins in insects. Dihydrofolate reductase and metallothionein-1 (MT-1) genes are present in high copy number in cultured mammalian cells subjected to low levels of agents that will select for cells with amplified copies of specific genes. Recent studies have shown that the metallothlonein-1 gene in mouse liver is regulated at the transcriptional level by treatment with heavy metals. We report here that, at cadmium concentrations 5 to 10-fold higher than that required to induce maximal transcription of the MT-1 gene, there is a 2 to 3-fold increase in MT-1 gene concentration in liver nuclear DNA by 6 hours after induction, and extra copies persist up to 3 weeks in the absence of further heavy metal treatment. The extra MT-1 gene copies that appear 6 hours after cadmium treatment are in a conformation that renders them relatively nuclease insensitive.
version4
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/76296600/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Koropatnick, J
1Winning, R
2Wiese, E
3Heschl, M
4Gedamu, L
5Duerksen, J
titleAcute treatment of mice with cadmium salts results in amplification of the metallothionein-1 gene in liver.
subject
0Animals–Pharmacology
1Cadmium–Drug Effects
2Cadmium Compounds–Metabolism
3Cell Nucleus–Genetics
4DNA–Metabolism
5DNA Replication–Drug Effects
6Gene Amplification–Drug Effects
7Kinetics–Ultrastructure
8Liver–Genetics
9Male–Pharmacology
10Metallothionein–Drug Effects
11Mice–Genetics
12Micrococcal Nuclease–Genetics
13Nucleic Acid Hybridization–Genetics
14Sulfates–Genetics
15Transcription, Genetic–Genetics
16Alpha-Fetoproteins–Genetics
17Cadmium Compounds
18Sulfates
19Alpha-Fetoproteins
20Cadmium
21DNA
22Metallothionein
23Cadmium Sulfate
24Micrococcal Nuclease
general
0English
1MEDLINE (ProQuest)
2ProQuest Biological Science Collection
3ProQuest Natural Science Collection
4ProQuest SciTech Collection
5Biological Science Database
6Natural Science Collection
7SciTech Premium Collection
8Health Research Premium Collection
9Health Research Premium Collection (Alumni edition)
10Biological Science Index (ProQuest)
sourceidproquest
recordidproquest76296600
issn
003051048
10305-1048
rsrctypearticle
creationdate1985
addtitleNucleic acids research
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorKoropatnick, J
startdate19850812
enddate19850812
citationpf 5423 pt 5439 vol 13 issue 15
lsr30VSR-Enriched:[doi, description, pqid, eissn]
sort
titleAcute treatment of mice with cadmium salts results in amplification of the metallothionein-1 gene in liver.
authorKoropatnick, J ; Winning, R ; Wiese, E ; Heschl, M ; Gedamu, L ; Duerksen, J
creationdate19850812
lso0119850812
facets
frbrgroupid2843809903309806059
frbrtype5
newrecords20181218
languageeng
creationdate1985
topic
0Animals–Pharmacology
1Cadmium–Drug Effects
2Cadmium Compounds–Metabolism
3Cell Nucleus–Genetics
4DNA–Metabolism
5DNA Replication–Drug Effects
6Gene Amplification–Drug Effects
7Kinetics–Ultrastructure
8Liver–Genetics
9Male–Pharmacology
10Metallothionein–Drug Effects
11Mice–Genetics
12Micrococcal Nuclease–Genetics
13Nucleic Acid Hybridization–Genetics
14Sulfates–Genetics
15Transcription, Genetic–Genetics
16Alpha-Fetoproteins–Genetics
17Cadmium Compounds
18Sulfates
19Alpha-Fetoproteins
20Cadmium
21DNA
22Metallothionein
23Cadmium Sulfate
24Micrococcal Nuclease
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Koropatnick, J
1Winning, R
2Wiese, E
3Heschl, M
4Gedamu, L
5Duerksen, J
jtitleNucleic acids research
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Koropatnick
1Winning
2Wiese
3Heschl
4Gedamu
5Duerksen
aufirst
0J
1R
2E
3M
4L
au
0Koropatnick, J
1Winning, R
2Wiese, E
3Heschl, M
4Gedamu, L
5Duerksen, J
addauKoropatnick, J
atitleAcute treatment of mice with cadmium salts results in amplification of the metallothionein-1 gene in liver.
jtitleNucleic acids research
risdate19850812
volume13
issue15
spage5423
epage5439
pages5423-5439
issn0305-1048
formatjournal
genrearticle
ristypeJOUR
urlhttp://search.proquest.com/docview/76296600/
doi10.1093/nar/13.15.5423
eissn13624962
date1985-08-12