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Studies on the optimal conditions for inactivation of Bordetella pertussis organisms with glutaraldehyde for preparation of a safe and potent pertussis vaccine.

The optimal conditions for inactivation of Bordetella pertussis organisms with glutaraldehyde for the production of a safe and potent whole cell pertussis vaccine were investigated. Two bacterial harvests from B. pertussis strain 10536 were treated with glutaraldehyde, each with 0.025, 0.05 and 0.1%... Full description

Journal Title: Vaccine December 1988, Vol.6(6), pp.491-496
Main Author: Gupta, R K
Other Authors: Saxena, S N , Sharma, S B , Ahuja, S
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0264-410X
Link: http://search.proquest.com/docview/78751626/?pq-origsite=primo
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title: Studies on the optimal conditions for inactivation of Bordetella pertussis organisms with glutaraldehyde for preparation of a safe and potent pertussis vaccine.
format: Article
creator:
  • Gupta, R K
  • Saxena, S N
  • Sharma, S B
  • Ahuja, S
subjects:
  • Aldehydes–Toxicity
  • Animals–Drug Effects
  • Biomarkers–Toxicity
  • Bordetella Pertussis–Adverse Effects
  • Glutaral–Toxicity
  • Histamine–Toxicity
  • Hot Temperature–Toxicity
  • Mice–Toxicity
  • Pertussis Vaccine–Toxicity
  • Vaccines, Inactivated–Toxicity
  • Weight Gain–Toxicity
  • Aldehydes
  • Biomarkers
  • Pertussis Vaccine
  • Vaccines, Inactivated
  • Histamine
  • Glutaral
ispartof: Vaccine, December 1988, Vol.6(6), pp.491-496
description: The optimal conditions for inactivation of Bordetella pertussis organisms with glutaraldehyde for the production of a safe and potent whole cell pertussis vaccine were investigated. Two bacterial harvests from B. pertussis strain 10536 were treated with glutaraldehyde, each with 0.025, 0.05 and 0.1% concentrations of glutaraldehyde for 10, 60 and 120 min. The nine types of glutaraldehyde-inactivated pertussis vaccine (GIPV) and conventional heat-inactivated pertussis vaccine (HIPV) preparations made from two bacterial harvests were comparatively evaluated for the mouse weight gain test (MWGT), potency, and the histamine-sensitization (HS) and leucocytosis-promoting-factor (LPF) tests. The minimum period for killing the B. pertussis organisms with glutaraldehyde was >10 min for 0.025%, 10 min for 0.05% and 5 min for 0.1% concentration. The average loss in opacity varied from 5 to 10% for GIPV preparations and was 14% for HIPV preparations. The GIPV preparations except those inactivated with 0.025% glutaraldehyde for 10 min (GIPV-A) were much less toxic than the HIPV preparations in the MWGT. The GIPV-A preparations did not pass the MWGT. The GIPV preparations were also much less toxic in HS and LPF tests than the HIPV preparation. The potency of GIPV preparations inactivated with 0.05% glutaraldehyde for 10 min (GIPV-D) was similar to that of HIPV preparations. The prolonged treatments with glutaraldehyde reduced the potency. The GIPV-D preparation with good potency and less toxicity was found to be inactivated with glutaraldehyde under optimal conditions. All the preparations were innocuous in the abnormal toxicity test.
language: eng
source:
identifier: ISSN: 0264-410X
fulltext: fulltext
issn:
  • 0264410X
  • 0264-410X
url: Link


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titleStudies on the optimal conditions for inactivation of Bordetella pertussis organisms with glutaraldehyde for preparation of a safe and potent pertussis vaccine.
creatorGupta, R K ; Saxena, S N ; Sharma, S B ; Ahuja, S
contributorGupta, R K (correspondence author) ; Gupta, R K (record owner)
ispartofVaccine, December 1988, Vol.6(6), pp.491-496
identifierISSN: 0264-410X
subjectAldehydes–Toxicity ; Animals–Drug Effects ; Biomarkers–Toxicity ; Bordetella Pertussis–Adverse Effects ; Glutaral–Toxicity ; Histamine–Toxicity ; Hot Temperature–Toxicity ; Mice–Toxicity ; Pertussis Vaccine–Toxicity ; Vaccines, Inactivated–Toxicity ; Weight Gain–Toxicity ; Aldehydes ; Biomarkers ; Pertussis Vaccine ; Vaccines, Inactivated ; Histamine ; Glutaral
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descriptionThe optimal conditions for inactivation of Bordetella pertussis organisms with glutaraldehyde for the production of a safe and potent whole cell pertussis vaccine were investigated. Two bacterial harvests from B. pertussis strain 10536 were treated with glutaraldehyde, each with 0.025, 0.05 and 0.1% concentrations of glutaraldehyde for 10, 60 and 120 min. The nine types of glutaraldehyde-inactivated pertussis vaccine (GIPV) and conventional heat-inactivated pertussis vaccine (HIPV) preparations made from two bacterial harvests were comparatively evaluated for the mouse weight gain test (MWGT), potency, and the histamine-sensitization (HS) and leucocytosis-promoting-factor (LPF) tests. The minimum period for killing the B. pertussis organisms with glutaraldehyde was >10 min for 0.025%, 10 min for 0.05% and 5 min for 0.1% concentration. The average loss in opacity varied from 5 to 10% for GIPV preparations and was 14% for HIPV preparations. The GIPV preparations except those inactivated with 0.025% glutaraldehyde for 10 min (GIPV-A) were much less toxic than the HIPV preparations in the MWGT. The GIPV-A preparations did not pass the MWGT. The GIPV preparations were also much less toxic in HS and LPF tests than the HIPV preparation. The potency of GIPV preparations inactivated with 0.05% glutaraldehyde for 10 min (GIPV-D) was similar to that of HIPV preparations. The prolonged treatments with glutaraldehyde reduced the potency. The GIPV-D preparation with good potency and less toxicity was found to be inactivated with glutaraldehyde under optimal conditions. All the preparations were innocuous in the abnormal toxicity test.
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titleStudies on the optimal conditions for inactivation of Bordetella pertussis organisms with glutaraldehyde for preparation of a safe and potent pertussis vaccine.
authorGupta, R K ; Saxena, S N ; Sharma, S B ; Ahuja, S
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