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Enzymatic dissection of the functions of the mouse egg's receptor for sperm.

During the course of sperm-egg interaction in mice, zona pellucida glycoprotein ZP3 (approximately equal to 80 kDa) serves as both receptor for sperm (J. D. Bleil and P. M. Wassarman, 1980c, Cell 20, 873-882) and inducer of the acrosome reaction (J. D. Bleil and P. M. Wassarman, 1983, Dev. Biol. 95,... Full description

Journal Title: Developmental biology November 1984, Vol.106(1), pp.243-255
Main Author: Florman, H M
Other Authors: Bechtol, K B , Wassarman, P M
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0012-1606
Link: http://search.proquest.com/docview/81296089/?pq-origsite=primo
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title: Enzymatic dissection of the functions of the mouse egg's receptor for sperm.
format: Article
creator:
  • Florman, H M
  • Bechtol, K B
  • Wassarman, P M
subjects:
  • Acrosome–Analysis
  • Animals–Analysis
  • Chromatography, Gel–Analysis
  • Egg Proteins–Analysis
  • Electrophoresis, Polyacrylamide Gel–Analysis
  • Female–Analysis
  • Fluorescent Antibody Technique–Analysis
  • Glycoproteins–Analysis
  • Male–Analysis
  • Membrane Glycoproteins–Analysis
  • Mice–Analysis
  • Receptors, Cell Surface–Analysis
  • Sperm-Ovum Interactions–Analysis
  • Zona Pellucida–Analysis
  • Zona Pellucida Glycoproteins–Analysis
  • Egg Proteins
  • Glycoproteins
  • Membrane Glycoproteins
  • Receptors, Cell Surface
  • Zona Pellucida Glycoproteins
ispartof: Developmental biology, November 1984, Vol.106(1), pp.243-255
description: During the course of sperm-egg interaction in mice, zona pellucida glycoprotein ZP3 (approximately equal to 80 kDa) serves as both receptor for sperm (J. D. Bleil and P. M. Wassarman, 1980c, Cell 20, 873-882) and inducer of the acrosome reaction (J. D. Bleil and P. M. Wassarman, 1983, Dev. Biol. 95, 317-324). In this investigation, small ZP3 glycopeptides (approximately equal to 1.5-6 kDa), obtained by extensive digestion of the purified glycoprotein with insoluble Pronase, were assayed for both sperm receptor and acrosome reaction-inducing activities. While ZP3 glycopeptides were virtually as effective as intact ZP3 in inhibiting binding of sperm to eggs in vitro ("receptor activity"), unlike intact ZP3, they failed to induce sperm to undergo the acrosome reaction. The latter was determined by indirect immunofluorescence using a monoclonal antibody directed against the acrosomal cap region of sperm. These results suggest that the sperm receptor activity of ZP3 is dependent only on its...
language: eng
source:
identifier: ISSN: 0012-1606
fulltext: fulltext
issn:
  • 00121606
  • 0012-1606
url: Link


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titleEnzymatic dissection of the functions of the mouse egg's receptor for sperm.
creatorFlorman, H M ; Bechtol, K B ; Wassarman, P M
contributorFlorman, H M (correspondence author) ; Florman, H M (record owner)
ispartofDevelopmental biology, November 1984, Vol.106(1), pp.243-255
identifierISSN: 0012-1606
subjectAcrosome–Analysis ; Animals–Analysis ; Chromatography, Gel–Analysis ; Egg Proteins–Analysis ; Electrophoresis, Polyacrylamide Gel–Analysis ; Female–Analysis ; Fluorescent Antibody Technique–Analysis ; Glycoproteins–Analysis ; Male–Analysis ; Membrane Glycoproteins–Analysis ; Mice–Analysis ; Receptors, Cell Surface–Analysis ; Sperm-Ovum Interactions–Analysis ; Zona Pellucida–Analysis ; Zona Pellucida Glycoproteins–Analysis ; Egg Proteins ; Glycoproteins ; Membrane Glycoproteins ; Receptors, Cell Surface ; Zona Pellucida Glycoproteins
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descriptionDuring the course of sperm-egg interaction in mice, zona pellucida glycoprotein ZP3 (approximately equal to 80 kDa) serves as both receptor for sperm (J. D. Bleil and P. M. Wassarman, 1980c, Cell 20, 873-882) and inducer of the acrosome reaction (J. D. Bleil and P. M. Wassarman, 1983, Dev. Biol. 95, 317-324). In this investigation, small ZP3 glycopeptides (approximately equal to 1.5-6 kDa), obtained by extensive digestion of the purified glycoprotein with insoluble Pronase, were assayed for both sperm receptor and acrosome reaction-inducing activities. While ZP3 glycopeptides were virtually as effective as intact ZP3 in inhibiting binding of sperm to eggs in vitro ("receptor activity"), unlike intact ZP3, they failed to induce sperm to undergo the acrosome reaction. The latter was determined by indirect immunofluorescence using a monoclonal antibody directed against the acrosomal cap region of sperm. These results suggest that the sperm receptor activity of ZP3 is dependent only on its...
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