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Thr-370 is responsible for CDK11(p58) autophosphorylation, dimerization, and kinase activity.

CDK11(p58), a member of the p34(cdc2)-related kinase family, is associated with cell cycle progression, tumorigenesis, and proapoptotic signaling. It is also required for the maintenance of chromosome cohesion, the maturation of centrosome, the formation of bipolar spindle, and the completion of mit... Full description

Journal Title: The Journal of biological chemistry January 21, 2011, Vol.286(3), pp.1748-1757
Main Author: Chi, Yayun
Other Authors: Zhang, Chunyi , Zong, Hongliang , Hong, Yi , Kong, Xiangfei , Liu, Haiou , Zou, Weiying , Wang, Yanlin , Yun, Xiaojing , Gu, Jianxin
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1083-351X ; DOI: 10.1074/jbc.M110.107367
Link: http://search.proquest.com/docview/843412650/?pq-origsite=primo
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title: Thr-370 is responsible for CDK11(p58) autophosphorylation, dimerization, and kinase activity.
format: Article
creator:
  • Chi, Yayun
  • Zhang, Chunyi
  • Zong, Hongliang
  • Hong, Yi
  • Kong, Xiangfei
  • Liu, Haiou
  • Zou, Weiying
  • Wang, Yanlin
  • Yun, Xiaojing
  • Gu, Jianxin
subjects:
  • Amino Acid Substitution–Physiology
  • Apoptosis–Genetics
  • Cyclin-Dependent Kinases–Metabolism
  • Hela Cells–Physiology
  • Humans–Physiology
  • Mutation, Missense–Physiology
  • Phosphorylation–Physiology
  • Protein Multimerization–Genetics
  • Signal Transduction–Metabolism
  • Transcriptional Activation–Metabolism
  • Tryptophan–Metabolism
  • Tryptophan
  • Cdk11a Protein, Human
  • Cyclin-Dependent Kinases
ispartof: The Journal of biological chemistry, January 21, 2011, Vol.286(3), pp.1748-1757
description: CDK11(p58), a member of the p34(cdc2)-related kinase family, is associated with cell cycle progression, tumorigenesis, and proapoptotic signaling. It is also required for the maintenance of chromosome cohesion, the maturation of centrosome, the formation of bipolar spindle, and the completion of mitosis. Here we identified that CDK11(p58) interacted with itself to form homodimers in cells, whereas D224N, the kinase-dead mutant, failed to form homodimers. CDK11(p58) was autophosphorylated, and the main functions of CDK11(p58), such as kinase activity, transactivation of nuclear receptors, and proapoptotic signal transduction, were dependent on its autophosphorylation. Furthermore, the in vitro kinase assay indicated that CDK11(p58) was autophosphorylated at Thr-370. By mutagenesis, we created CDK11(p58) T370A and CDK11(p58) T370D, which mimic the dephosphorylated and phosphorylated forms of CDK11(p58), respectively. The T370A mutant could not form dimers and be phosphorylated by the wild type CDK11(p58) and finally lost the kinase activity. Further functional research revealed that T370A failed to repress the transactivation of androgen receptor and enhance the cell apoptosis. Overall, our data indicated that Thr-370 is responsible for the autophosphorylation, dimerization, and kinase activity of CDK11(p58). Moreover, Thr-370 mutants might affect CDK11(p58)-mediated signaling pathways.
language: eng
source:
identifier: E-ISSN: 1083-351X ; DOI: 10.1074/jbc.M110.107367
fulltext: fulltext
issn:
  • 1083351X
  • 1083-351X
url: Link


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titleThr-370 is responsible for CDK11(p58) autophosphorylation, dimerization, and kinase activity.
creatorChi, Yayun ; Zhang, Chunyi ; Zong, Hongliang ; Hong, Yi ; Kong, Xiangfei ; Liu, Haiou ; Zou, Weiying ; Wang, Yanlin ; Yun, Xiaojing ; Gu, Jianxin
contributorChi, Yayun (correspondence author) ; Chi, Yayun (record owner)
ispartofThe Journal of biological chemistry, January 21, 2011, Vol.286(3), pp.1748-1757
identifierE-ISSN: 1083-351X ; DOI: 10.1074/jbc.M110.107367
subjectAmino Acid Substitution–Physiology ; Apoptosis–Genetics ; Cyclin-Dependent Kinases–Metabolism ; Hela Cells–Physiology ; Humans–Physiology ; Mutation, Missense–Physiology ; Phosphorylation–Physiology ; Protein Multimerization–Genetics ; Signal Transduction–Metabolism ; Transcriptional Activation–Metabolism ; Tryptophan–Metabolism ; Tryptophan ; Cdk11a Protein, Human ; Cyclin-Dependent Kinases
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descriptionCDK11(p58), a member of the p34(cdc2)-related kinase family, is associated with cell cycle progression, tumorigenesis, and proapoptotic signaling. It is also required for the maintenance of chromosome cohesion, the maturation of centrosome, the formation of bipolar spindle, and the completion of mitosis. Here we identified that CDK11(p58) interacted with itself to form homodimers in cells, whereas D224N, the kinase-dead mutant, failed to form homodimers. CDK11(p58) was autophosphorylated, and the main functions of CDK11(p58), such as kinase activity, transactivation of nuclear receptors, and proapoptotic signal transduction, were dependent on its autophosphorylation. Furthermore, the in vitro kinase assay indicated that CDK11(p58) was autophosphorylated at Thr-370. By mutagenesis, we created CDK11(p58) T370A and CDK11(p58) T370D, which mimic the dephosphorylated and phosphorylated forms of CDK11(p58), respectively. The T370A mutant could not form dimers and be phosphorylated by the wild type CDK11(p58) and finally lost the kinase activity. Further functional research revealed that T370A failed to repress the transactivation of androgen receptor and enhance the cell apoptosis. Overall, our data indicated that Thr-370 is responsible for the autophosphorylation, dimerization, and kinase activity of CDK11(p58). Moreover, Thr-370 mutants might affect CDK11(p58)-mediated signaling pathways.
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titleThr-370 is responsible for CDK11(p58) autophosphorylation, dimerization, and kinase activity.
authorChi, Yayun ; Zhang, Chunyi ; Zong, Hongliang ; Hong, Yi ; Kong, Xiangfei ; Liu, Haiou ; Zou, Weiying ; Wang, Yanlin ; Yun, Xiaojing ; Gu, Jianxin
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