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Dual-emission fluorescent silica nanoparticle-based probe for ultrasensitive detection of Cu2+.

An effective dual-emission fluorescent silica nanoparticle based probe has been constructed for rapid and ultrasensitive detection of [Cu.sup.2+]. In this nanoprobe, a dye-doped silica core served as a signal, thus providing a built-in correction for environmental effects. A response dye was covalen... Full description

Journal Title: Analytical chemistry April 15, 2011, Vol.83(8), pp.3126-3132
Main Author: Zong, Chenghua
Other Authors: Ai, Kelong , Zhang, Guo , Li, Hongwei , Lu, Lehui
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; DOI: 10.1021/ac2001324
Link: http://search.proquest.com/docview/862279303/?pq-origsite=primo
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title: Dual-emission fluorescent silica nanoparticle-based probe for ultrasensitive detection of Cu2+.
format: Article
creator:
  • Zong, Chenghua
  • Ai, Kelong
  • Zhang, Guo
  • Li, Hongwei
  • Lu, Lehui
subjects:
  • Cell Survival–Drug Effects
  • Copper–Analysis
  • Dose-Response Relationship, Drug–Chemistry
  • Fluorescence–Pharmacology
  • Fluorescent Dyes–Chemistry
  • Fresh Water–Chemistry
  • Hela Cells–Chemistry
  • Humans–Chemistry
  • Microscopy, Fluorescence–Chemistry
  • Molecular Structure–Chemistry
  • Nanoparticles–Chemistry
  • Particle Size–Chemistry
  • Sensitivity and Specificity–Chemistry
  • Silicon Dioxide–Chemistry
  • Structure-Activity Relationship–Chemistry
  • Surface Properties–Chemistry
  • Water Pollutants, Chemical–Chemistry
  • Fluorescent Dyes
  • Water Pollutants, Chemical
  • Silicon Dioxide
  • Copper
ispartof: Analytical chemistry, April 15, 2011, Vol.83(8), pp.3126-3132
description: An effective dual-emission fluorescent silica nanoparticle based probe has been constructed for rapid and ultrasensitive detection of [Cu.sup.2+]. In this nanoprobe, a dye-doped silica core served as a signal, thus providing a built-in correction for environmental effects. A response dye was covalently grafted on the surface of the silica nanoparticles through a chelating reagent for [Cu.sup.2+]. The fluorescence of the response dye could be selectively quenched in the presence of [Cu.sup.2+], accompanied by a visual orange-to green color switch of the nanoprobe. The nanoprobe provided an effective platform for reliable detection of [Cu.sup.2+] with a detection limit as low as 10 nM, which is nearly 2 x [10.sup.3] times lower than the maximum level (~20 [micro]M) of [Cu.sup.2+] in drinking water permitted by the U.S. Environmental Protection Agency (EPA). The high sensitivity was attributed to the strong chelation of [Cu.sup.2+] with polyethyleneimine (PEI) and a signal amplification effect. The nanoprobe constructed by this method was very stable, enabling the rapid detection of Cu2 in real water samples. Good linear correlations were obtained over the concentration range from 1 x [10.sup.-7] to 8 [10.sup.-7] ([R.sup.2] = 0.99) with recoveries of 103.8-99.14% and 95.5-95.14% for industrial wastewater and lake water, respectively. Additionally, the long-wavelength emission of the response dye can avoid the interference of the autofluorescence of the biosystems, which facilitated their applications in monitoring [Cu.sup.2+] in cells. Furthermore, the nanoprobe showed a good reversibility; the fluorescence can be switched "off" and "on" by an addition of [Cu.sup.2+] and EDTA, respectively. dx.doi.org/ 10.1021/ac2001324
language: eng
source:
identifier: E-ISSN: 1520-6882 ; DOI: 10.1021/ac2001324
fulltext: fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleDual-emission fluorescent silica nanoparticle-based probe for ultrasensitive detection of Cu2+.
creatorZong, Chenghua ; Ai, Kelong ; Zhang, Guo ; Li, Hongwei ; Lu, Lehui
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ispartofAnalytical chemistry, April 15, 2011, Vol.83(8), pp.3126-3132
identifierE-ISSN: 1520-6882 ; DOI: 10.1021/ac2001324
subjectCell Survival–Drug Effects ; Copper–Analysis ; Dose-Response Relationship, Drug–Chemistry ; Fluorescence–Pharmacology ; Fluorescent Dyes–Chemistry ; Fresh Water–Chemistry ; Hela Cells–Chemistry ; Humans–Chemistry ; Microscopy, Fluorescence–Chemistry ; Molecular Structure–Chemistry ; Nanoparticles–Chemistry ; Particle Size–Chemistry ; Sensitivity and Specificity–Chemistry ; Silicon Dioxide–Chemistry ; Structure-Activity Relationship–Chemistry ; Surface Properties–Chemistry ; Water Pollutants, Chemical–Chemistry ; Fluorescent Dyes ; Water Pollutants, Chemical ; Silicon Dioxide ; Copper
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descriptionAn effective dual-emission fluorescent silica nanoparticle based probe has been constructed for rapid and ultrasensitive detection of [Cu.sup.2+]. In this nanoprobe, a dye-doped silica core served as a signal, thus providing a built-in correction for environmental effects. A response dye was covalently grafted on the surface of the silica nanoparticles through a chelating reagent for [Cu.sup.2+]. The fluorescence of the response dye could be selectively quenched in the presence of [Cu.sup.2+], accompanied by a visual orange-to green color switch of the nanoprobe. The nanoprobe provided an effective platform for reliable detection of [Cu.sup.2+] with a detection limit as low as 10 nM, which is nearly 2 x [10.sup.3] times lower than the maximum level (~20 [micro]M) of [Cu.sup.2+] in drinking water permitted by the U.S. Environmental Protection Agency (EPA). The high sensitivity was attributed to the strong chelation of [Cu.sup.2+] with polyethyleneimine (PEI) and a signal amplification effect. The nanoprobe constructed by this method was very stable, enabling the rapid detection of Cu2 in real water samples. Good linear correlations were obtained over the concentration range from 1 x [10.sup.-7] to 8 [10.sup.-7] ([R.sup.2] = 0.99) with recoveries of 103.8-99.14% and 95.5-95.14% for industrial wastewater and lake water, respectively. Additionally, the long-wavelength emission of the response dye can avoid the interference of the autofluorescence of the biosystems, which facilitated their applications in monitoring [Cu.sup.2+] in cells. Furthermore, the nanoprobe showed a good reversibility; the fluorescence can be switched "off" and "on" by an addition of [Cu.sup.2+] and EDTA, respectively. dx.doi.org/ 10.1021/ac2001324
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