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Development of a UHPLC method for the assessment of the metabolic profile of cinitapride.

An ultra high‐performance liquid chromatographic method was developed to study the cinitapride metabolism. Metabolites were generated from the incubation of cinitapride with human liver microsomes. Cinitapride and its metabolites were separated by reversed‐phase mode using a formate aqueous solution... Full description

Journal Title: Journal of separation science December 2011, Vol.34(24), pp.3502-3508
Main Author: Marquez, Helena
Other Authors: Albertí, Joan , Salvà, Miquel , Saurina, Javier , Sentellas, Sonia
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1615-9314 ; DOI: 10.1002/jssc.201100073
Link: http://search.proquest.com/docview/911939883/?pq-origsite=primo
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title: Development of a UHPLC method for the assessment of the metabolic profile of cinitapride.
format: Article
creator:
  • Marquez, Helena
  • Albertí, Joan
  • Salvà, Miquel
  • Saurina, Javier
  • Sentellas, Sonia
subjects:
  • Benzamides–Analysis
  • Chromatography, High Pressure Liquid–Metabolism
  • Humans–Pharmacology
  • Microsomes, Liver–Drug Effects
  • Microsomes, Liver–Metabolism
  • Benzamides
  • Cinitapride
ispartof: Journal of separation science, December 2011, Vol.34(24), pp.3502-3508
description: An ultra high‐performance liquid chromatographic method was developed to study the cinitapride metabolism. Metabolites were generated from the incubation of cinitapride with human liver microsomes. Cinitapride and its metabolites were separated by reversed‐phase mode using a formate aqueous solution (pH 6.5) and acetonitrile as the components of the mobile phase. Chromatographic conditions, including the establishment of an elution gradient, were optimized for obtaining the maximum number of resolved components in the minimum analysis time. Experimental design and multicriteria decision‐making strategies were utilized to facilitate the optimization of chromatographic conditions. Figures of merit were evaluated with cinitapride standards and incubated samples. Limits of detection are about 0.03 μmol/L, and repeatabilities are better than 0.06% for retention times and better than 3.5% for concentrations. The method was applied to characterize the in vitro cinitapride metabolism with human liver microsomes. ; p. 3502-3508.
language: eng
source:
identifier: E-ISSN: 1615-9314 ; DOI: 10.1002/jssc.201100073
fulltext: fulltext
issn:
  • 16159314
  • 1615-9314
url: Link


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titleDevelopment of a UHPLC method for the assessment of the metabolic profile of cinitapride.
creatorMarquez, Helena ; Albertí, Joan ; Salvà, Miquel ; Saurina, Javier ; Sentellas, Sonia
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identifierE-ISSN: 1615-9314 ; DOI: 10.1002/jssc.201100073
subjectBenzamides–Analysis ; Chromatography, High Pressure Liquid–Metabolism ; Humans–Pharmacology ; Microsomes, Liver–Drug Effects ; Microsomes, Liver–Metabolism ; Benzamides ; Cinitapride
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descriptionAn ultra high‐performance liquid chromatographic method was developed to study the cinitapride metabolism. Metabolites were generated from the incubation of cinitapride with human liver microsomes. Cinitapride and its metabolites were separated by reversed‐phase mode using a formate aqueous solution (pH 6.5) and acetonitrile as the components of the mobile phase. Chromatographic conditions, including the establishment of an elution gradient, were optimized for obtaining the maximum number of resolved components in the minimum analysis time. Experimental design and multicriteria decision‐making strategies were utilized to facilitate the optimization of chromatographic conditions. Figures of merit were evaluated with cinitapride standards and incubated samples. Limits of detection are about 0.03 μmol/L, and repeatabilities are better than 0.06% for retention times and better than 3.5% for concentrations. The method was applied to characterize the in vitro cinitapride metabolism with human liver microsomes. ; p. 3502-3508.
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