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Active transcription and essential role of RNA polymerase II at the centromere during mitosis.

Transcription of the centromeric regions has been reported to occur in G1 and S phase in different species. Here, we investigate whether transcription also occurs and plays a functional role at the mammalian centromere during mitosis. We show the presence of actively transcribing RNA polymerase II (... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America February 7, 2012, Vol.109(6), pp.1979-1984
Main Author: Chan, F Lyn
Other Authors: Marshall, Owen J , Saffery, Richard , Kim, Bo Won , Earle, Elizabeth , Choo, K H Andy , Wong, Lee H
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1108705109
Link: http://search.proquest.com/docview/921143853/?pq-origsite=primo
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title: Active transcription and essential role of RNA polymerase II at the centromere during mitosis.
format: Article
creator:
  • Chan, F Lyn
  • Marshall, Owen J
  • Saffery, Richard
  • Kim, Bo Won
  • Earle, Elizabeth
  • Choo, K H Andy
  • Wong, Lee H
subjects:
  • Animals–Metabolism
  • Centromere–Metabolism
  • Chromosomal Proteins, Non-Histone–Metabolism
  • Chromosomes, Mammalian–Metabolism
  • DNA, Satellite–Metabolism
  • DNA-Binding Proteins–Metabolism
  • Gene Expression Regulation–Metabolism
  • Hela Cells–Genetics
  • Humans–Metabolism
  • Kinetochores–Metabolism
  • Mice–Chemistry
  • Mitosis–Metabolism
  • Phosphoprotein Phosphatases–Metabolism
  • Protein Transport–Metabolism
  • RNA Polymerase II–Metabolism
  • RNA, Messenger–Metabolism
  • Serine–Metabolism
  • Transcription Factors–Metabolism
  • Transcription, Genetic–Metabolism
  • Chromosomal Proteins, Non-Histone
ispartof: Proceedings of the National Academy of Sciences of the United States of America, February 7, 2012, Vol.109(6), pp.1979-1984
description: Transcription of the centromeric regions has been reported to occur in G1 and S phase in different species. Here, we investigate whether transcription also occurs and plays a functional role at the mammalian centromere during mitosis. We show the presence of actively transcribing RNA polymerase II (RNAPII) and its associated transcription factors, coupled with the production of centromere satellite transcripts at the mitotic kinetochore. Specific inhibition of RNAPII activity during mitosis leads to a decrease in centromeric a-satellite transcription and a concomitant increase in anaphase-lagging cells, with the lagging chromosomes showing reduced centromere protein C binding. These findings demonstrate an essential role of RNAPII in the transcription of a-satellite DNA, binding of centromere protein C, and the proper functioning of the mitotic kinetochore. chromatin | noncoding RNA | epigenetics doi/ 10.1073/pnas.1108705109
language: eng
source:
identifier: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1108705109
fulltext: fulltext
issn:
  • 10916490
  • 1091-6490
url: Link


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titleActive transcription and essential role of RNA polymerase II at the centromere during mitosis.
creatorChan, F Lyn ; Marshall, Owen J ; Saffery, Richard ; Kim, Bo Won ; Earle, Elizabeth ; Choo, K H Andy ; Wong, Lee H
contributorChan, F Lyn (correspondence author) ; Chan, F Lyn (record owner)
ispartofProceedings of the National Academy of Sciences of the United States of America, February 7, 2012, Vol.109(6), pp.1979-1984
identifierE-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1108705109
subjectAnimals–Metabolism ; Centromere–Metabolism ; Chromosomal Proteins, Non-Histone–Metabolism ; Chromosomes, Mammalian–Metabolism ; DNA, Satellite–Metabolism ; DNA-Binding Proteins–Metabolism ; Gene Expression Regulation–Metabolism ; Hela Cells–Genetics ; Humans–Metabolism ; Kinetochores–Metabolism ; Mice–Chemistry ; Mitosis–Metabolism ; Phosphoprotein Phosphatases–Metabolism ; Protein Transport–Metabolism ; RNA Polymerase II–Metabolism ; RNA, Messenger–Metabolism ; Serine–Metabolism ; Transcription Factors–Metabolism ; Transcription, Genetic–Metabolism ; Chromosomal Proteins, Non-Histone
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descriptionTranscription of the centromeric regions has been reported to occur in G1 and S phase in different species. Here, we investigate whether transcription also occurs and plays a functional role at the mammalian centromere during mitosis. We show the presence of actively transcribing RNA polymerase II (RNAPII) and its associated transcription factors, coupled with the production of centromere satellite transcripts at the mitotic kinetochore. Specific inhibition of RNAPII activity during mitosis leads to a decrease in centromeric a-satellite transcription and a concomitant increase in anaphase-lagging cells, with the lagging chromosomes showing reduced centromere protein C binding. These findings demonstrate an essential role of RNAPII in the transcription of a-satellite DNA, binding of centromere protein C, and the proper functioning of the mitotic kinetochore. chromatin | noncoding RNA | epigenetics doi/ 10.1073/pnas.1108705109
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date2012-02-07