schliessen

Filtern

 

Bibliotheken

Probing transient Hoogsteen hydrogen bonds in canonical duplex DNA using NMR relaxation dispersion and single-atom substitution.

Nucleic acids transiently morph into alternative conformations that can be difficult to characterize at the atomic level by conventional methods because they exist for too little time and in too little abundance. We recently reported evidence for transient Hoogsteen (HG) base pairs in canonical B-DN... Full description

Journal Title: Journal of the American Chemical Society February 29, 2012, Vol.134(8), pp.3667-3670
Main Author: Nikolova, Evgenia N
Other Authors: Gottardo, Federico L , Al-Hashimi, Hashim M
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: E-ISSN: 1520-5126 ; DOI: 10.1021/ja2117816
Link: http://search.proquest.com/docview/925716309/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest925716309
title: Probing transient Hoogsteen hydrogen bonds in canonical duplex DNA using NMR relaxation dispersion and single-atom substitution.
format: Article
creator:
  • Nikolova, Evgenia N
  • Gottardo, Federico L
  • Al-Hashimi, Hashim M
subjects:
  • DNA–Chemistry
  • Hydrogen Bonding–Chemistry
  • Nuclear Magnetic Resonance, Biomolecular–Chemistry
  • Nucleic Acid Conformation–Chemistry
  • DNA
ispartof: Journal of the American Chemical Society, February 29, 2012, Vol.134(8), pp.3667-3670
description: Nucleic acids transiently morph into alternative conformations that can be difficult to characterize at the atomic level by conventional methods because they exist for too little time and in too little abundance. We recently reported evidence for transient Hoogsteen (HG) base pairs in canonical B-DNA based on NMR carbon relaxation dispersion. While the carbon chemical shifts measured for the transient state were consistent with a syn orientation for the purine base, as expected for A(syn)•T(anti) and G(syn)•C(+)(anti) HG base pairing, HG type hydrogen bonding could only be inferred indirectly. Here, we develop two independent approaches for directly probing transient changes in N-H···N hydrogen bonds and apply them to the characterization of transient Hoogsteen type hydrogen bonds in canonical duplex DNA. The first approach takes advantage of the strong dependence of the imino nitrogen chemical shift on hydrogen bonding and involves measurement of R(1ρ) relaxation dispersion for the hydrogen-bond donor imino nitrogens in G and T residues. In the second approach, we assess the consequence of substituting the hydrogen-bond acceptor nitrogen (N7) with a carbon (C7H7) on both carbon and nitrogen relaxation dispersion data. Together, these data allow us to obtain direct evidence for transient Hoogsteen base pairs that are stabilized by N-H···N type hydrogen bonds in canonical duplex DNA. The methods introduced here greatly expand the utility of NMR in the structural characterization of transient states in nucleic acids.
language: eng
source:
identifier: E-ISSN: 1520-5126 ; DOI: 10.1021/ja2117816
fulltext: fulltext
issn:
  • 15205126
  • 1520-5126
url: Link


@attributes
ID477180268
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid925716309
sourceidproquest
recordidTN_proquest925716309
sourcesystemPC
pqid925716309
galeid287674931
display
typearticle
titleProbing transient Hoogsteen hydrogen bonds in canonical duplex DNA using NMR relaxation dispersion and single-atom substitution.
creatorNikolova, Evgenia N ; Gottardo, Federico L ; Al-Hashimi, Hashim M
contributorNikolova, Evgenia N (correspondence author) ; Nikolova, Evgenia N (record owner)
ispartofJournal of the American Chemical Society, February 29, 2012, Vol.134(8), pp.3667-3670
identifierE-ISSN: 1520-5126 ; DOI: 10.1021/ja2117816
subjectDNA–Chemistry ; Hydrogen Bonding–Chemistry ; Nuclear Magnetic Resonance, Biomolecular–Chemistry ; Nucleic Acid Conformation–Chemistry ; DNA
languageeng
source
descriptionNucleic acids transiently morph into alternative conformations that can be difficult to characterize at the atomic level by conventional methods because they exist for too little time and in too little abundance. We recently reported evidence for transient Hoogsteen (HG) base pairs in canonical B-DNA based on NMR carbon relaxation dispersion. While the carbon chemical shifts measured for the transient state were consistent with a syn orientation for the purine base, as expected for A(syn)•T(anti) and G(syn)•C(+)(anti) HG base pairing, HG type hydrogen bonding could only be inferred indirectly. Here, we develop two independent approaches for directly probing transient changes in N-H···N hydrogen bonds and apply them to the characterization of transient Hoogsteen type hydrogen bonds in canonical duplex DNA. The first approach takes advantage of the strong dependence of the imino nitrogen chemical shift on hydrogen bonding and involves measurement of R(1ρ) relaxation dispersion for the hydrogen-bond donor imino nitrogens in G and T residues. In the second approach, we assess the consequence of substituting the hydrogen-bond acceptor nitrogen (N7) with a carbon (C7H7) on both carbon and nitrogen relaxation dispersion data. Together, these data allow us to obtain direct evidence for transient Hoogsteen base pairs that are stabilized by N-H···N type hydrogen bonds in canonical duplex DNA. The methods introduced here greatly expand the utility of NMR in the structural characterization of transient states in nucleic acids.
version4
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/925716309/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Nikolova, Evgenia N
1Gottardo, Federico L
2Al-Hashimi, Hashim M
titleProbing transient Hoogsteen hydrogen bonds in canonical duplex DNA using NMR relaxation dispersion and single-atom substitution.
subject
0DNA–Chemistry
1Hydrogen Bonding–Chemistry
2Nuclear Magnetic Resonance, Biomolecular–Chemistry
3Nucleic Acid Conformation–Chemistry
4DNA
general
0English
110.1021/ja2117816
2MEDLINE (ProQuest)
3ProQuest Biological Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Biological Science Database
7Natural Science Collection
8SciTech Premium Collection
9Health Research Premium Collection
10Health Research Premium Collection (Alumni edition)
11Biological Science Index (ProQuest)
sourceidproquest
recordidproquest925716309
issn
015205126
11520-5126
rsrctypearticle
creationdate2012
addtitleJournal of the American Chemical Society
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorNikolova, Evgenia N
startdate20120229
enddate20120229
citationpf 3667 pt 3670 vol 134 issue 8
lsr30VSR-Enriched:[galeid, pqid, issn, description]
sort
titleProbing transient Hoogsteen hydrogen bonds in canonical duplex DNA using NMR relaxation dispersion and single-atom substitution.
authorNikolova, Evgenia N ; Gottardo, Federico L ; Al-Hashimi, Hashim M
creationdate20120229
lso0120120229
facets
frbrgroupid1515026298947416822
frbrtype5
newrecords20181218
languageeng
creationdate2012
topic
0DNA–Chemistry
1Hydrogen Bonding–Chemistry
2Nuclear Magnetic Resonance, Biomolecular–Chemistry
3Nucleic Acid Conformation–Chemistry
4DNA
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Nikolova, Evgenia N
1Gottardo, Federico L
2Al-Hashimi, Hashim M
jtitleJournal of the American Chemical Society
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Nikolova
1Gottardo
2Al-Hashimi
aufirst
0Evgenia N
1Federico L
2Hashim M
au
0Nikolova, Evgenia N
1Gottardo, Federico L
2Al-Hashimi, Hashim M
addauNikolova, Evgenia N
atitleProbing transient Hoogsteen hydrogen bonds in canonical duplex DNA using NMR relaxation dispersion and single-atom substitution.
jtitleJournal of the American Chemical Society
risdate20120229
volume134
issue8
spage3667
epage3670
pages3667-3670
eissn1520-5126
formatjournal
genrearticle
ristypeJOUR
doi10.1021/ja2117816
urlhttp://search.proquest.com/docview/925716309/
issn00027863
date2012-02-29