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Toxicological assessment of TiO 2 nanoparticles by recombinant Escherichia coli bacteria

Rapid and efficient methods to assess nanoparticle toxicity are desired in current research. Here we showed that Escherichia coli labeled by green fluorescent protein can be a good model bacterium for assessing acute toxicity of TiO 2 (about 50% inhibition ratios after 135 min exposure). Sodium dode... Full description

Journal Title: Journal of Environmental Monitoring 2011, Vol.13(1), pp.42-48
Main Author: Jiang, Guoxiang
Other Authors: Shen, Zhenyao , Niu, Junfeng , Bao, Yueping , Chen, Jing , He, Tiande
Format: Electronic Article Electronic Article
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ID: ISSN: 1464-0325 ; E-ISSN: 1464-0333 ; DOI: 10.1039/c0em00499e
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recordid: rscc0em00499e
title: Toxicological assessment of TiO 2 nanoparticles by recombinant Escherichia coli bacteria
format: Article
creator:
  • Jiang, Guoxiang
  • Shen, Zhenyao
  • Niu, Junfeng
  • Bao, Yueping
  • Chen, Jing
  • He, Tiande
subjects:
  • Environmental Monitoring
  • Fluorescence
  • Reactive Oxygen Species
  • Post-Translation
  • Humic Acids
  • Green Fluorescent Protein
  • Chromophores
  • Acute Toxicity
  • Nanoparticles
  • Gel Electrophoresis
  • Sodium
  • Acute Toxicity
  • Oxygen
  • Electrophoresis
  • Fluorescence
  • Humic Acids
  • Proteins
  • Toxicity
  • Escherichia Coli
  • Toxicology And Health
  • Toxicology & Environmental Safety
  • Environmental Pollution & Waste Treatment
  • Bacteria
  • Biochemistry
  • Methods
ispartof: Journal of Environmental Monitoring, 2011, Vol.13(1), pp.42-48
description: Rapid and efficient methods to assess nanoparticle toxicity are desired in current research. Here we showed that Escherichia coli labeled by green fluorescent protein can be a good model bacterium for assessing acute toxicity of TiO 2 (about 50% inhibition ratios after 135 min exposure). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that TiO 2 nanoparticles (NPs) can influence certain protein expression in the recombinant bacterium, and the obvious effects in repressed expression and elevated expression were observed in 30/40, 10/20 μg mL −1 treated cells, respectively. However, the GFP expression (27 kD) was not influenced by introduced TiO 2 NPs. The change of the fluorescence intensity may be caused by the damage in folding and chromophore formation of the GFP post-translational modification due to generated reactive oxygen species. Furthermore, TiO 2 NPs at higher concentrations decreased their toxicity because of aggregation. 20 μg mL −1 humic acid (HA) introduced to the medium can decrease the fluorescent inhibition owing to the barrier of steric hindrance it provides between NPs and cells.
language:
source:
identifier: ISSN: 1464-0325 ; E-ISSN: 1464-0333 ; DOI: 10.1039/c0em00499e
fulltext: no_fulltext
issn:
  • 1464-0325
  • 1464-0333
  • 14640333
  • 14640325
url: Link


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titleToxicological assessment of TiO 2 nanoparticles by recombinant Escherichia coli bacteria
creatorJiang, Guoxiang ; Shen, Zhenyao ; Niu, Junfeng ; Bao, Yueping ; Chen, Jing ; He, Tiande
ispartofJournal of Environmental Monitoring, 2011, Vol.13(1), pp.42-48
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descriptionRapid and efficient methods to assess nanoparticle toxicity are desired in current research. Here we showed that Escherichia coli labeled by green fluorescent protein can be a good model bacterium for assessing acute toxicity of TiO 2 (about 50% inhibition ratios after 135 min exposure). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that TiO 2 nanoparticles (NPs) can influence certain protein expression in the recombinant bacterium, and the obvious effects in repressed expression and elevated expression were observed in 30/40, 10/20 μg mL −1 treated cells, respectively. However, the GFP expression (27 kD) was not influenced by introduced TiO 2 NPs. The change of the fluorescence intensity may be caused by the damage in folding and chromophore formation of the GFP post-translational modification due to generated reactive oxygen species. Furthermore, TiO 2 NPs at higher concentrations decreased their toxicity because of aggregation. 20 μg mL −1 humic acid (HA) introduced to the medium can decrease the fluorescent inhibition owing to the barrier of steric hindrance it provides between NPs and cells.
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subjectEnvironmental Monitoring ; Fluorescence ; Reactive Oxygen Species ; Post-Translation ; Humic Acids ; Green Fluorescent Protein ; Chromophores ; Acute Toxicity ; Nanoparticles ; Gel Electrophoresis ; Sodium ; Acute Toxicity ; Oxygen ; Electrophoresis ; Fluorescence ; Humic Acids ; Proteins ; Toxicity ; Escherichia Coli ; Toxicology And Health ; Toxicology & Environmental Safety ; Environmental Pollution & Waste Treatment ; Bacteria ; Biochemistry ; Methods;
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descriptionRapid and efficient methods to assess nanoparticle toxicity are desired in current research. Here we showed that Escherichia coli labeled by green fluorescent protein can be a good model bacterium for assessing acute toxicity of TiO 2 (about 50% inhibition ratios after 135 min exposure). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that TiO 2 nanoparticles (NPs) can influence certain protein expression in the recombinant bacterium, and the obvious effects in repressed expression and elevated expression were observed in 30/40, 10/20 μg mL −1 treated cells, respectively. However, the GFP expression (27 kD) was not influenced by introduced TiO 2 NPs. The change of the fluorescence intensity may be caused by the damage in folding and chromophore formation of the GFP post-translational modification due to generated reactive oxygen species. Furthermore, TiO 2 NPs at higher concentrations decreased their toxicity because of aggregation. 20 μg mL −1 humic acid (HA) introduced to the medium can decrease the fluorescent inhibition owing to the barrier of steric hindrance it provides between NPs and cells.
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abstractRapid and efficient methods to assess nanoparticle toxicity are desired in current research. Here we showed that Escherichia coli labeled by green fluorescent protein can be a good model bacterium for assessing acute toxicity of TiO 2 (about 50% inhibition ratios after 135 min exposure). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that TiO 2 nanoparticles (NPs) can influence certain protein expression in the recombinant bacterium, and the obvious effects in repressed expression and elevated expression were observed in 30/40, 10/20 μg mL −1 treated cells, respectively. However, the GFP expression (27 kD) was not influenced by introduced TiO 2 NPs. The change of the fluorescence intensity may be caused by the damage in folding and chromophore formation of the GFP post-translational modification due to generated reactive oxygen species. Furthermore, TiO 2 NPs at higher concentrations decreased their toxicity because of aggregation. 20 μg mL −1 humic acid (HA) introduced to the medium can decrease the fluorescent inhibition owing to the barrier of steric hindrance it provides between NPs and cells.
doi10.1039/c0em00499e
pages42-48
date2011-01-12