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A genetically encoded aldehyde for rapid protein labelling

Using a mutant pyrrolysyl-tRNA synthetase-tRNA Pyl CUA pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli . This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.

Journal Title: Chemical Communications 2014, Vol.50(56), pp.7424-7426
Main Author: Tuley, Alfred
Other Authors: Lee, Yan-Jiun , Wu, Bo , Wang, Zhiyong U. , Liu, Wenshe R.
Format: Electronic Article Electronic Article
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ID: ISSN: 1359-7345 ; E-ISSN: 1364-548X ; DOI: 10.1039/c4cc02000f
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recordid: rscc4cc02000f
title: A genetically encoded aldehyde for rapid protein labelling
format: Article
creator:
  • Tuley, Alfred
  • Lee, Yan-Jiun
  • Wu, Bo
  • Wang, Zhiyong U.
  • Liu, Wenshe R.
subjects:
  • Labelling
  • Amino Acids
  • Encoding
  • Dyes
  • Mutations
  • Escherichia Coli
  • Proteins
  • Aldehydes
  • Miscellaneous Sciences (So)
  • Components and Materials (General) (Ea)
ispartof: Chemical Communications, 2014, Vol.50(56), pp.7424-7426
description: Using a mutant pyrrolysyl-tRNA synthetase-tRNA Pyl CUA pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli . This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
language:
source:
identifier: ISSN: 1359-7345 ; E-ISSN: 1364-548X ; DOI: 10.1039/c4cc02000f
fulltext: fulltext
issn:
  • 1359-7345
  • 1364-548X
  • 1364548X
  • 13597345
url: Link


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descriptionUsing a mutant pyrrolysyl-tRNA synthetase-tRNA Pyl CUA pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli . This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
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subjectLabelling ; Amino Acids ; Encoding ; Dyes ; Mutations ; Escherichia Coli ; Proteins ; Aldehydes ; Miscellaneous Sciences (So) ; Components and Materials (General) (Ea);
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titleA genetically encoded aldehyde for rapid protein labelling
descriptionUsing a mutant pyrrolysyl-tRNA synthetase-tRNA Pyl CUA pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli . This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
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authorTuley, Alfred ; Lee, Yan-Jiun ; Wu, Bo ; Wang, Zhiyong U. ; Liu, Wenshe R.
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abstractUsing a mutant pyrrolysyl-tRNA synthetase-tRNA Pyl CUA pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli . This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
doi10.1039/c4cc02000f
pages7424-7426
date2014-06-17