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Preparation of protein imprinted polymer beads by Pickering emulsion polymerization

We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water... Full description

Journal Title: Journal of Materials Chemistry B 2015, Vol.3(7), pp.1254-1260
Main Author: Zhou, Tongchang
Other Authors: Zhang, Ka , Kamra, Tripta , Blow, Leif , Ye, Lei
Format: Electronic Article Electronic Article
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ID: DOI: 10.1039/c4tb01605j
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recordid: rscc4tb01605j
title: Preparation of protein imprinted polymer beads by Pickering emulsion polymerization
format: Article
creator:
  • Zhou, Tongchang
  • Zhang, Ka
  • Kamra, Tripta
  • Blow, Leif
  • Ye, Lei
subjects:
  • Naturvetenskap
  • Biologi
  • Biokemi Och Molekylärbiologi
  • Natural Sciences
  • Biological Sciences
  • Biochemistry And Molecular Biology
  • Naturvetenskap
  • Fysik
  • Atom- Och Molekylfysik Och Optik
  • Natural Sciences
  • Physical Sciences
  • Atom And Molecular Physics And Optics
ispartof: Journal of Materials Chemistry B, 2015, Vol.3(7), pp.1254-1260
description: We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.
language:
source:
identifier: DOI: 10.1039/c4tb01605j
fulltext: fulltext
issn:
  • 2050-750X
  • 2050-7518
  • 20507518
  • 2050750X
url: Link


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titlePreparation of protein imprinted polymer beads by Pickering emulsion polymerization
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identifierDOI: 10.1039/c4tb01605j
descriptionWe present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.
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subjectNaturvetenskap ; Biologi ; Biokemi Och Molekylärbiologi ; Natural Sciences ; Biological Sciences ; Biochemistry And Molecular Biology ; Naturvetenskap ; Fysik ; Atom- Och Molekylfysik Och Optik ; Natural Sciences ; Physical Sciences ; Atom And Molecular Physics And Optics;
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titlePreparation of protein imprinted polymer beads by Pickering emulsion polymerization
descriptionWe present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.
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abstractWe present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.
doi10.1039/c4tb01605j
pages1254-1260
date2015-02-04