schliessen

Filtern

 

Bibliotheken

A biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher

An efficient ferrocenyl BODIPY based dark quencher has been developed and employed to construct a FRET-based fluorescent probe that contains a biotin moiety as a potential directing ligand for cancer cells and a glutathione-cleavable disulfide linker connecting the quencher and a distyryl BODIPY flu... Full description

Journal Title: Dalton Transactions Dalton Transactions, 2016, Vol.45(44), pp.17798-17806
Main Author: Shi, Wen-Jing
Other Authors: Lo, Pui-Chi , Zhao, Shirui , Wong, Roy C. H. , Wang, Qiong , Fong, Wing-Ping , Ng, Dennis K. P.
Format: Electronic Article Electronic Article
Language:
Subjects:
ID: ISSN: 1477-9239 ; E-ISSN: 1477-9247 ; DOI: 10.1039/c6dt03141b
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: rscc6dt03141b
title: A biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
format: Article
creator:
  • Shi, Wen-Jing
  • Lo, Pui-Chi
  • Zhao, Shirui
  • Wong, Roy C. H.
  • Wang, Qiong
  • Fong, Wing-Ping
  • Ng, Dennis K. P.
subjects:
  • Biotin -- Chemistry
  • Boron Compounds -- Chemistry
  • Fluorescent Dyes -- Chemistry
  • Glutathione -- Analysis
ispartof: Dalton Transactions, Dalton Transactions, 2016, Vol.45(44), pp.17798-17806
description: An efficient ferrocenyl BODIPY based dark quencher has been developed and employed to construct a FRET-based fluorescent probe that contains a biotin moiety as a potential directing ligand for cancer cells and a glutathione-cleavable disulfide linker connecting the quencher and a distyryl BODIPY fluorophore. This molecular probe is deactivated in the native form through FRET followed by intramolecular charge transfer due to the ferrocenyl unit. However, upon interaction with glutathione in phosphate buffered saline and inside cancer cells, the fluorescence emission is significantly increased due to detachment of the fluorophore from the quencher. As shown by flow cytometry, this probe also exhibits preferential uptake by the biotin-receptor-expressing A549 human lung adenocarcinoma epithelial cells over the Chinese hamster ovary CHO-K1 cells used as the negative control. On the basis that both biotin receptor and GSH level are often overexpressed or elevated in cancer cells, this dual functional fluorescent probe serves as a promising agent for cancer imaging.
language:
source:
identifier: ISSN: 1477-9239 ; E-ISSN: 1477-9247 ; DOI: 10.1039/c6dt03141b
fulltext: fulltext
issn:
  • 1477-9226
  • 1477-9234
  • 14779234
  • 14779226
url: Link


@attributes
ID1768338088
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidc6dt03141b
sourceidrsc
recordidTN_rscc6dt03141b
sourceformatXML
sourcesystemPC
pqid1864528378
display
typearticle
titleA biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
creatorShi, Wen-Jing ; Lo, Pui-Chi ; Zhao, Shirui ; Wong, Roy C. H. ; Wang, Qiong ; Fong, Wing-Ping ; Ng, Dennis K. P.
ispartofDalton Transactions, Dalton Transactions, 2016, Vol.45(44), pp.17798-17806
identifier
descriptionAn efficient ferrocenyl BODIPY based dark quencher has been developed and employed to construct a FRET-based fluorescent probe that contains a biotin moiety as a potential directing ligand for cancer cells and a glutathione-cleavable disulfide linker connecting the quencher and a distyryl BODIPY fluorophore. This molecular probe is deactivated in the native form through FRET followed by intramolecular charge transfer due to the ferrocenyl unit. However, upon interaction with glutathione in phosphate buffered saline and inside cancer cells, the fluorescence emission is significantly increased due to detachment of the fluorophore from the quencher. As shown by flow cytometry, this probe also exhibits preferential uptake by the biotin-receptor-expressing A549 human lung adenocarcinoma epithelial cells over the Chinese hamster ovary CHO-K1 cells used as the negative control. On the basis that both biotin receptor and GSH level are often overexpressed or elevated in cancer cells, this dual functional fluorescent probe serves as a promising agent for cancer imaging.
source
subjectBiotin -- Chemistry ; Boron Compounds -- Chemistry ; Fluorescent Dyes -- Chemistry ; Glutathione -- Analysis;
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Shi, Wen-Jing
1Lo, Pui-Chi
2Zhao, Shirui
3Wong, Roy C. H.
4Wang, Qiong
5Fong, Wing-Ping
6Ng, Dennis K. P.
titleA biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
descriptionAn efficient ferrocenyl BODIPY based dark quencher has been developed and employed to construct a FRET-based fluorescent probe that contains a biotin moiety as a potential directing ligand for cancer cells and a glutathione-cleavable disulfide linker connecting the quencher and a distyryl BODIPY fluorophore. This molecular probe is deactivated in the native form through FRET followed by intramolecular charge transfer due to the ferrocenyl unit. However, upon interaction with glutathione in phosphate buffered saline and inside cancer cells, the fluorescence emission is significantly increased due to detachment of the fluorophore from the quencher. As shown by flow cytometry, this probe also exhibits preferential uptake by the biotin-receptor-expressing A549 human lung adenocarcinoma epithelial cells over the Chinese hamster ovary CHO-K1 cells used as the negative control. On the basis that both biotin receptor and GSH level are often overexpressed or elevated in cancer cells, this dual functional fluorescent probe serves as a promising agent for cancer imaging.
general
010.1039/c6dt03141b
1Royal Society of Chemistry
sourceidrsc
recordidrscc6dt03141b
issn
01477-9226
11477-9234
214779234
314779226
rsrctypearticle
creationdate2016
addtitle
0Dalton Transactions
1Dalton Trans.
2Journal of the Chemical Society, Dalton Transactions
searchscope
0RSC
1rsc
scope
0RSC
1rsc
lsr30VSR-Enriched:[pages, subject, pqid]
sort
titleA biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
authorShi, Wen-Jing ; Lo, Pui-Chi ; Zhao, Shirui ; Wong, Roy C. H. ; Wang, Qiong ; Fong, Wing-Ping ; Ng, Dennis K. P.
creationdate20161108
facets
frbrgroupid3548590745272433798
frbrtype5
creationdate2016
collectionRSC Journals (Royal Society of Chemistry)
prefilterarticles
rsrctypearticles
creatorcontrib
0Shi,Wen-Jing
1Lo,Pui-Chi
2Zhao,Shirui
3Wong,Roy C. H.
4Wang,Qiong
5Fong,Wing-Ping
6Ng,Dennis K. P.
jtitleDalton Transactions
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Shi
1Lo
2Zhao
3Wong
4Wang
5Fong
6Ng
aufirst
0Wen-Jing
1Pui-Chi
2Shirui
3Roy C. H.
4Qiong
5Wing-Ping
6Dennis K. P.
au
0Shi, Wen-Jing
1Lo, Pui-Chi
2Zhao, Shirui
3Wong, Roy C. H.
4Wang, Qiong
5Fong, Wing-Ping
6Ng, Dennis K. P.
atitleA biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
jtitle
0Dalton Transactions
1Journal of the Chemical Society, Dalton Transactions
addtitleDalton Trans.
risdate20161108
volume45
issue44
spage17798
epage17806
issn1477-9226
eissn1477-9234
genrearticle
ristypeJOUR
abstractAn efficient ferrocenyl BODIPY based dark quencher has been developed and employed to construct a FRET-based fluorescent probe that contains a biotin moiety as a potential directing ligand for cancer cells and a glutathione-cleavable disulfide linker connecting the quencher and a distyryl BODIPY fluorophore. This molecular probe is deactivated in the native form through FRET followed by intramolecular charge transfer due to the ferrocenyl unit. However, upon interaction with glutathione in phosphate buffered saline and inside cancer cells, the fluorescence emission is significantly increased due to detachment of the fluorophore from the quencher. As shown by flow cytometry, this probe also exhibits preferential uptake by the biotin-receptor-expressing A549 human lung adenocarcinoma epithelial cells over the Chinese hamster ovary CHO-K1 cells used as the negative control. On the basis that both biotin receptor and GSH level are often overexpressed or elevated in cancer cells, this dual functional fluorescent probe serves as a promising agent for cancer imaging.
doi10.1039/c6dt03141b
pages17798-17806
date2016-11-08