schliessen

Filtern

 

Bibliotheken

Expression of Receptors for Corticotropin-Releasing Factor in the Vasculature of Pregnant Rats

Objective: To identify and localize the receptor(s) responsible for modulating vascular effects of corticotropin-releasing factor (CRF) during pregnancy. Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR), competitive RT-PCR, and Western blot analyses were used to study the expression... Full description

Journal Title: Journal of the Society for Gynecologic Investigation May 2000, Vol.7(3), pp.153-160
Main Author: Jain, Venu
Other Authors: Longo, Monica , Ali, Mariam , Saade, George R , Chwalisz, Kristof , Garfield, Robert E
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1071-5576 ; E-ISSN: 1556-7117 ; DOI: 10.1177/107155760000700303
Link: https://journals.sagepub.com/doi/full/10.1177/107155760000700303
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: sage_s10_1177_107155760000700303
title: Expression of Receptors for Corticotropin-Releasing Factor in the Vasculature of Pregnant Rats
format: Article
creator:
  • Jain, Venu
  • Longo, Monica
  • Ali, Mariam
  • Saade, George R
  • Chwalisz, Kristof
  • Garfield, Robert E
subjects:
  • Corticotropin-Releasing Factor Receptor
  • Pregnant Rat
  • Aorta
  • Uterine Artery
  • Endothelium
  • Medicine
ispartof: Journal of the Society for Gynecologic Investigation, May 2000, Vol.7(3), pp.153-160
description: Objective: To identify and localize the receptor(s) responsible for modulating vascular effects of corticotropin-releasing factor (CRF) during pregnancy. Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR), competitive RT-PCR, and Western blot analyses were used to study the expression of CRF receptors (CRFR1, CRFR2α, and CRFR2β) in the aorta and uterine vascular bed of nonpregnant and late (day 18) and term pregnant (day 22) Sprague-Dawley rats. Immunohistochemistry was done to localize the CRF receptor in the aortic wall. There were six rats in each study group. Results: Only CRFR2β was identified in the aorta and uterine vascular bed by RT-PCR and Western blot analyses. The PCR product was sequenced to confirm its identity. Competitive RT-PCR and Western blot analyses showed that expression of CRFR2β is not different in late pregnancy compared with the nonpregnant but is decreased at term. Immunohistochemistry showed...
language: eng
source:
identifier: ISSN: 1071-5576 ; E-ISSN: 1556-7117 ; DOI: 10.1177/107155760000700303
fulltext: no_fulltext
issn:
  • 1071-5576
  • 10715576
  • 1556-7117
  • 15567117
url: Link


@attributes
ID1488954185
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10_1177_107155760000700303
sourceidsage_s
recordidTN_sage_s10_1177_107155760000700303
sourcesystemPC
dbid
0.2E
1.2J
2.2N
331R
431U
531X
631Z
7AACTG
8AAGMC
9AAMPI
10AAQDB
11AARDL
12AATBZ
13AAUAS
14ABEIX
15ABJUO
16ABQXT
17ACDXX
18ACGEY
19ACGZU
20ACJTF
21ADGDL
22AECGH
23AEDTQ
24AEKYL
25AERKM
26AGWFA
27AMCVQ
28B3H
29B8R
30B8Z
31B94
32BKSCU
33DB0
34DF0
35DO-
36J8X
37Q7L
38Q7U
39Q7X
40Q81
41Q83
42SCNPE
43SDB
44SFC
45SFK
46SFT
47SGO
48SGR
49SGV
50SGZ
51SNB
52SPJ
53STM
pqid71228057
display
typearticle
titleExpression of Receptors for Corticotropin-Releasing Factor in the Vasculature of Pregnant Rats
creatorJain, Venu ; Longo, Monica ; Ali, Mariam ; Saade, George R ; Chwalisz, Kristof ; Garfield, Robert E
ispartofJournal of the Society for Gynecologic Investigation, May 2000, Vol.7(3), pp.153-160
identifier
subjectCorticotropin-Releasing Factor Receptor ; Pregnant Rat ; Aorta ; Uterine Artery ; Endothelium ; Medicine
descriptionObjective: To identify and localize the receptor(s) responsible for modulating vascular effects of corticotropin-releasing factor (CRF) during pregnancy. Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR), competitive RT-PCR, and Western blot analyses were used to study the expression of CRF receptors (CRFR1, CRFR2α, and CRFR2β) in the aorta and uterine vascular bed of nonpregnant and late (day 18) and term pregnant (day 22) Sprague-Dawley rats. Immunohistochemistry was done to localize the CRF receptor in the aortic wall. There were six rats in each study group. Results: Only CRFR2β was identified in the aorta and uterine vascular bed by RT-PCR and Western blot analyses. The PCR product was sequenced to confirm its identity. Competitive RT-PCR and Western blot analyses showed that expression of CRFR2β is not different in late pregnancy compared with the nonpregnant but is decreased at term. Immunohistochemistry showed...
languageeng
source
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttps://journals.sagepub.com/doi/full/10.1177/107155760000700303$$EView_record_in_Sage_(Access_to_full_text_may_be_restricted)
search
creatorcontrib
0Jain, Venu
1Longo, Monica
2Ali, Mariam
3Saade, George R
4Chwalisz, Kristof
5Garfield, Robert E
titleExpression of Receptors for Corticotropin-Releasing Factor in the Vasculature of Pregnant Rats
description

Objective: To identify and localize the receptor(s) responsible for modulating vascular effects of corticotropin-releasing factor (CRF) during pregnancy. Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR), competitive RT-PCR, and Western blot analyses were used to study the expression of CRF receptors (CRFR1, CRFR2α, and CRFR2β) in the aorta and uterine vascular bed of nonpregnant and late (day 18) and term pregnant (day 22) Sprague-Dawley rats. Immunohistochemistry was done to localize the CRF receptor in the aortic wall. There were six rats in each study group. Results: Only CRFR2β was identified in the aorta and uterine vascular bed by RT-PCR and Western blot analyses. The PCR product was sequenced to confirm its identity. Competitive RT-PCR and Western blot analyses showed that expression of CRFR2β is not different in late pregnancy compared with the nonpregnant but is decreased at term. Immunohistochemistry showed...

subject
0Corticotropin-Releasing Factor Receptor
1Pregnant Rat
2Aorta
3Uterine Artery
4Endothelium
5Medicine
general
0English
1Sage Publications
210.1177/107155760000700303
3Sage Journals (Sage Publications)
4SAGE Clinical Medicine (Sage Publications)
5SAGE Health Sciences (Sage Publications)
6SAGE STM (Sage Publications)
7SAGE Journals (Sage Publications)
sourceidsage_s
recordidsage_s10_1177_107155760000700303
issn
01071-5576
110715576
21556-7117
315567117
rsrctypearticle
creationdate2000
addtitleJournal of the Society for Gynecologic Investigation
searchscope
0sage_full
1sage24
2sage131
3sage252
4sage21
5sage128
6sage210
7sage249
8sage254
9sage27
10sage219
11sage15
12sage139
13sage255
14sage29
15sage238
16sage220
17sage221
18sage216
19sage134
20sage28
21sage257
22sage135
23sage133
24sage26
25sage138
26sage256
27sage136
28sage22
29sage129
30sage211
31sage250
32sage30
33sage25
34sage132
35sage253
36sage42
37sage130
38sage23
39sage54
40sage212
41sage251
42sage31
43sage19
44sage11
45sage208
46sage206
47sage20
48sage127
49sage209
50sage248
51sage126
52sage207
53sage247
scope
0sage_full
1sage24
2sage131
3sage252
4sage21
5sage128
6sage210
7sage249
8sage254
9sage27
10sage219
11sage15
12sage139
13sage255
14sage29
15sage238
16sage220
17sage221
18sage216
19sage134
20sage28
21sage257
22sage135
23sage133
24sage26
25sage138
26sage256
27sage136
28sage22
29sage129
30sage211
31sage250
32sage30
33sage25
34sage132
35sage253
36sage42
37sage130
38sage23
39sage54
40sage212
41sage251
42sage31
43sage19
44sage11
45sage208
46sage206
47sage20
48sage127
49sage209
50sage248
51sage126
52sage207
53sage247
lsr44$$EView_record_in_Sage_(Access_to_full_text_may_be_restricted)
tmp01
0SAGE Clinical Medicine (Sage Publications)
1SAGE Health Sciences (Sage Publications)
2SAGE STM (Sage Publications)
3Sage Journals (Sage Publications)
4SAGE Journals (Sage Publications)
tmp02
0.2E
1.2J
2.2N
331R
431U
531X
631Z
7AACTG
8AAGMC
9AAMPI
10AAQDB
11AARDL
12AATBZ
13AAUAS
14ABEIX
15ABJUO
16ABQXT
17ACDXX
18ACGEY
19ACGZU
20ACJTF
21ADGDL
22AECGH
23AEDTQ
24AEKYL
25AERKM
26AGWFA
27AMCVQ
28B3H
29B8R
30B8Z
31B94
32BKSCU
33DB0
34DF0
35DO-
36J8X
37Q7L
38Q7U
39Q7X
40Q81
41Q83
42SCNPE
43SDB
44SFC
45SFK
46SFT
47SGO
48SGR
49SGV
50SGZ
51SNB
52SPJ
53STM
startdate20000501
enddate20000531
lsr40Journal of the Society for Gynecologic Investigation, May 2000, Vol.7 (3), pp.153-160
doi10.1177/107155760000700303
citationpf 153 pt 160 vol 7 issue 3
lsr30VSR-Enriched:[pqid]
sort
titleExpression of Receptors for Corticotropin-Releasing Factor in the Vasculature of Pregnant Rats
authorJain, Venu ; Longo, Monica ; Ali, Mariam ; Saade, George R ; Chwalisz, Kristof ; Garfield, Robert E
creationdate20000500
lso0120000500
facets
frbrgroupid9205970597723578352
frbrtype5
newrecords20170607
languageeng
topic
0Corticotropin-Releasing Factor Receptor
1Pregnant Rat
2Aorta
3Uterine Artery
4Endothelium
5Medicine
collectionSage Journals (Sage Publications)
prefilterarticles
rsrctypearticles
creatorcontrib
0Jain, Venu
1Longo, Monica
2Ali, Mariam
3Saade, George R
4Chwalisz, Kristof
5Garfield, Robert E
jtitleJournal of the Society for Gynecologic Investigation
creationdate2000
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
aulast
0Jain
1Longo
2Ali
3Saade
4Chwalisz
5Garfield
aufirst
0Venu
1Monica
2Mariam
3George R
4Kristof
5Robert E
auinitV
auinit1V
au
0Jain, Venu
1Longo, Monica
2Ali, Mariam
3Saade, George R
4Chwalisz, Kristof
5Garfield, Robert E
atitleExpression of Receptors for Corticotropin-Releasing Factor in the Vasculature of Pregnant Rats
jtitleJournal of the Society for Gynecologic Investigation
risdate200005
volume7
issue3
spage153
epage160
pages153-160
issn1071-5576
eissn1556-7117
formatjournal
genrearticle
ristypeJOUR
abstract

Objective: To identify and localize the receptor(s) responsible for modulating vascular effects of corticotropin-releasing factor (CRF) during pregnancy. Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR), competitive RT-PCR, and Western blot analyses were used to study the expression of CRF receptors (CRFR1, CRFR2α, and CRFR2β) in the aorta and uterine vascular bed of nonpregnant and late (day 18) and term pregnant (day 22) Sprague-Dawley rats. Immunohistochemistry was done to localize the CRF receptor in the aortic wall. There were six rats in each study group. Results: Only CRFR2β was identified in the aorta and uterine vascular bed by RT-PCR and Western blot analyses. The PCR product was sequenced to confirm its identity. Competitive RT-PCR and Western blot analyses showed that expression of CRFR2β is not different in late pregnancy compared with the nonpregnant but is decreased at term. Immunohistochemistry showed...

copThousand Oaks, CA
pubSage Publications
doi10.1177/107155760000700303
lad01Journal of the Society for Gynecologic Investigation
date2000-05