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Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children

The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disea... Full description

Journal Title: Memórias do Instituto Oswaldo Cruz 01 May 2010, Vol.105(3), pp.310-313
Main Author: Fraga, Thiago Leite
Other Authors: Brustoloni, Yvone Maia , Lima, Rosimar Baptista , Dorval, Maria Elizabeth Cavalheiros , Oshiro, Elisa Teruya , Oliveira, Janaina , Oliveira, Ana Lúcia Lyrio de , Pirmez, Claude
Format: Electronic Article Electronic Article
Language: Portugiesisch
Subjects:
Pcr
ID: ISSN: 1678-8060 ; E-ISSN: 1678-8060 ; DOI: 10.1590/S0074-02762010000300011
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title: Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
format: Article
creator:
  • Fraga, Thiago Leite
  • Brustoloni, Yvone Maia
  • Lima, Rosimar Baptista
  • Dorval, Maria Elizabeth Cavalheiros
  • Oshiro, Elisa Teruya
  • Oliveira, Janaina
  • Oliveira, Ana Lúcia Lyrio de
  • Pirmez, Claude
subjects:
  • Parasitology
  • Tropical Medicine
  • Leishmaniasis
  • Pcr
  • Peripheral Blood
  • Children
  • Medicine
ispartof: Memórias do Instituto Oswaldo Cruz, 01 May 2010, Vol.105(3), pp.310-313
description: The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests.
language: por
source:
identifier: ISSN: 1678-8060 ; E-ISSN: 1678-8060 ; DOI: 10.1590/S0074-02762010000300011
fulltext: fulltext_linktorsrc
issn:
  • 1678-8060
  • 16788060
url: Link


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titlePolymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
creatorFraga, Thiago Leite ; Brustoloni, Yvone Maia ; Lima, Rosimar Baptista ; Dorval, Maria Elizabeth Cavalheiros ; Oshiro, Elisa Teruya ; Oliveira, Janaina ; Oliveira, Ana Lúcia Lyrio de ; Pirmez, Claude
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subjectParasitology ; Tropical Medicine ; Leishmaniasis ; Pcr ; Peripheral Blood ; Children ; Medicine
descriptionThe diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests.
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titlePolymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
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The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests.

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The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests.

pubInstituto Oswaldo Cruz, Ministério da Saúde
doi10.1590/S0074-02762010000300011
urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300011&lng=en&tlng=en
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date2010-05-01