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Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts

This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pr... Full description

Journal Title: Brazilian Journal of Microbiology 01 December 2002, Vol.33(4), pp.339-341
Main Author: Ribeiro, Aldemir Reginato
Other Authors: Nascimento, Vladimir Pinheiro Do , Cardoso, Martha Oliveira , Santos, Luciana Ruschel Dos , Rocha, Silvio Luís Da Silveira
Format: Electronic Article Electronic Article
Language: Portugiesisch
Subjects:
ID: ISSN: 1678-4405 ; DOI: 10.1590/S1517-83822002000400012
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recordid: scielo_sS1517_83822002000400012
title: Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
format: Article
creator:
  • Ribeiro, Aldemir Reginato
  • Nascimento, Vladimir Pinheiro Do
  • Cardoso, Martha Oliveira
  • Santos, Luciana Ruschel Dos
  • Rocha, Silvio Luís Da Silveira
subjects:
  • Microbiology
  • Separação Imunomagnética
  • Salmonella
  • Partes de Frango
  • Immunomagnetic Separation
  • Salmonella
  • Raw Broiler Parts
ispartof: Brazilian Journal of Microbiology, 01 December 2002, Vol.33(4), pp.339-341
description: This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.
language: por
source:
identifier: ISSN: 1678-4405 ; DOI: 10.1590/S1517-83822002000400012
fulltext: fulltext_linktorsrc
issn:
  • 1678-4405
  • 16784405
url: Link


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titleUtilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
creatorRibeiro, Aldemir Reginato ; Nascimento, Vladimir Pinheiro Do ; Cardoso, Martha Oliveira ; Santos, Luciana Ruschel Dos ; Rocha, Silvio Luís Da Silveira
ispartofBrazilian Journal of Microbiology, 01 December 2002, Vol.33(4), pp.339-341
identifierISSN: 1678-4405 ; DOI: 10.1590/S1517-83822002000400012
subjectMicrobiology ; Separação Imunomagnética ; Salmonella ; Partes de Frango ; Immunomagnetic Separation ; Salmonella ; Raw Broiler Parts
descriptionThis study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.
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titleUtilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
description

This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.

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titleUtilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
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This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.

pubSociedade Brasileira de Microbiologia
doi10.1590/S1517-83822002000400012
urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400012&lng=en&tlng=en
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