schliessen

Filtern

 

Bibliotheken

Cartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells

Human embryonic stem cells (hESCs) have the potential to offer a virtually unlimited source of chondrogenic cells for use in cartilage repair and regeneration. We have recently shown that expandable chondrogenic cells can be derived from hESCs under selective growth factor-responsive conditions. In... Full description

Journal Title: Biomaterials 2010, Vol.31(27), pp.6968-6980
Main Author: Toh, Wei Seong
Other Authors: Lee, Eng Hin , Guo, Xi-Min , Chan, Jerry K.Y. , Yeow, Chen Hua , Choo, Andre B. , Cao, Tong
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0142-9612 ; DOI: 10.1016/j.biomaterials.2010.05.064
Link: http://dx.doi.org/10.1016/j.biomaterials.2010.05.064
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: sciversesciencedirect_elsevierS0142-9612(10)00717-9
title: Cartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells
format: Article
creator:
  • Toh, Wei Seong
  • Lee, Eng Hin
  • Guo, Xi-Min
  • Chan, Jerry K.Y.
  • Yeow, Chen Hua
  • Choo, Andre B.
  • Cao, Tong
subjects:
  • Human Embryonic Stem Cells
  • Chondrogenic
  • Cartilage
  • Differentiation
  • Hyaluronan
  • Hydrogel
ispartof: Biomaterials, 2010, Vol.31(27), pp.6968-6980
description: Human embryonic stem cells (hESCs) have the potential to offer a virtually unlimited source of chondrogenic cells for use in cartilage repair and regeneration. We have recently shown that expandable chondrogenic cells can be derived from hESCs under selective growth factor-responsive conditions. In this study, we explore the potential of these hESC-derived chondrogenic cells to produce an extracellular matrix (ECM)-enriched cartilaginous tissue construct when cultured in hyaluronic acid (HA)-based hydrogel, and further investigated the long-term reparative ability of the resulting hESC-derived chondrogenic cell-engineered cartilage (HCCEC) in an osteochondral defect model. We hypothesized that HCCEC can provide a functional template capable of undergoing orderly remodeling during the repair of critical-sized osteochondral defects (1.5 mm in diameter, 1 mm depth into the subchondral bone) in a rat model. In the process of repair, we observed an orderly spatial-temporal remodeling of HCCEC over 12 weeks into osteochondral tissue, with characteristic architectural features including a hyaline-like neocartilage layer with good surface regularity and complete integration with the adjacent host cartilage and a regenerated subchondral bone. By 12 weeks, the HCCEC-regenerated osteochondral tissue resembled closely that of age-matched unoperated native control, while only fibrous tissue filled in the control defects which left empty or treated with hydrogel alone. Here we demonstrate that transplanted hESC-derived chondrogenic cells maintain long-term viability with no evidence of tumorigenicity, providing a safe, highly-efficient and practical strategy of applying hESCs for cartilage tissue engineering.
language: eng
source:
identifier: ISSN: 0142-9612 ; DOI: 10.1016/j.biomaterials.2010.05.064
fulltext: fulltext
issn:
  • 01429612
  • 0142-9612
url: Link


@attributes
ID253991844
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordidS0142-9612(10)00717-9
sourceidsciversesciencedirect_elsevier
recordidTN_sciversesciencedirect_elsevierS0142-9612(10)00717-9
sourcesystemOther
pqid733977756
display
typearticle
titleCartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells
creatorToh, Wei Seong ; Lee, Eng Hin ; Guo, Xi-Min ; Chan, Jerry K.Y. ; Yeow, Chen Hua ; Choo, Andre B. ; Cao, Tong
ispartofBiomaterials, 2010, Vol.31(27), pp.6968-6980
identifierISSN: 0142-9612 ; DOI: 10.1016/j.biomaterials.2010.05.064
subjectHuman Embryonic Stem Cells ; Chondrogenic ; Cartilage ; Differentiation ; Hyaluronan ; Hydrogel
descriptionHuman embryonic stem cells (hESCs) have the potential to offer a virtually unlimited source of chondrogenic cells for use in cartilage repair and regeneration. We have recently shown that expandable chondrogenic cells can be derived from hESCs under selective growth factor-responsive conditions. In this study, we explore the potential of these hESC-derived chondrogenic cells to produce an extracellular matrix (ECM)-enriched cartilaginous tissue construct when cultured in hyaluronic acid (HA)-based hydrogel, and further investigated the long-term reparative ability of the resulting hESC-derived chondrogenic cell-engineered cartilage (HCCEC) in an osteochondral defect model. We hypothesized that HCCEC can provide a functional template capable of undergoing orderly remodeling during the repair of critical-sized osteochondral defects (1.5 mm in diameter, 1 mm depth into the subchondral bone) in a rat model. In the process of repair, we observed an orderly spatial-temporal remodeling of HCCEC over 12 weeks into osteochondral tissue, with characteristic architectural features including a hyaline-like neocartilage layer with good surface regularity and complete integration with the adjacent host cartilage and a regenerated subchondral bone. By 12 weeks, the HCCEC-regenerated osteochondral tissue resembled closely that of age-matched unoperated native control, while only fibrous tissue filled in the control defects which left empty or treated with hydrogel alone. Here we demonstrate that transplanted hESC-derived chondrogenic cells maintain long-term viability with no evidence of tumorigenicity, providing a safe, highly-efficient and practical strategy of applying hESCs for cartilage tissue engineering.
languageeng
source
version6
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://dx.doi.org/10.1016/j.biomaterials.2010.05.064$$EView_record_in_ScienceDirect
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Toh, Wei Seong
1Lee, Eng Hin
2Guo, Xi-Min
3Chan, Jerry K.Y.
4Yeow, Chen Hua
5Choo, Andre B.
6Cao, Tong
titleCartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells
descriptionHuman embryonic stem cells (hESCs) have the potential to offer a virtually unlimited source of chondrogenic cells for use in cartilage repair and regeneration. We have recently shown that expandable chondrogenic cells can be derived from hESCs under selective growth factor-responsive conditions. In this study, we explore the potential of these hESC-derived chondrogenic cells to produce an extracellular matrix (ECM)-enriched cartilaginous tissue construct when cultured in hyaluronic acid (HA)-based hydrogel, and further investigated the long-term reparative ability of the resulting hESC-derived chondrogenic cell-engineered cartilage (HCCEC) in an osteochondral defect model. We hypothesized that HCCEC can provide a functional template capable of undergoing orderly remodeling during the repair of critical-sized osteochondral defects (1.5 mm in diameter, 1 mm depth into the subchondral bone) in a rat model. In the process of repair, we observed an orderly spatial-temporal remodeling of HCCEC over 12 weeks into osteochondral tissue, with characteristic architectural features including a hyaline-like neocartilage layer with good surface regularity and complete integration with the adjacent host cartilage and a regenerated subchondral bone. By 12 weeks, the HCCEC-regenerated osteochondral tissue resembled closely that of age-matched unoperated native control, while only fibrous tissue filled in the control defects which left empty or treated with hydrogel alone. Here we demonstrate that transplanted hESC-derived chondrogenic cells maintain long-term viability with no evidence of tumorigenicity, providing a safe, highly-efficient and practical strategy of applying hESCs for cartilage tissue engineering.
subject
0Human Embryonic Stem Cells
1Chondrogenic
2Cartilage
3Differentiation
4Hyaluronan
5Hydrogel
general
0English
1Elsevier Ltd
210.1016/j.biomaterials.2010.05.064
3ScienceDirect (Elsevier B.V.)
4ScienceDirect Journals (Elsevier)
sourceidsciversesciencedirect_elsevier
recordidsciversesciencedirect_elsevierS0142-9612(10)00717-9
issn
001429612
10142-9612
rsrctypearticle
creationdate2010
addtitleBiomaterials
searchscope
0sciversesciencedirect_elsevier
1elsevier_sciencedirect
scope
0sciversesciencedirect_elsevier
1elsevier_sciencedirect
startdate20100101
enddate20101231
citationpf 6968 pt 6980 vol 31 issue 27
lsr30VSR-Enriched:[pqid, eissn]
sort
titleCartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells
authorToh, Wei Seong ; Lee, Eng Hin ; Guo, Xi-Min ; Chan, Jerry K.Y. ; Yeow, Chen Hua ; Choo, Andre B. ; Cao, Tong
creationdate20100000
lso0120100000
facets
frbrgroupid5502316592749372888
frbrtype5
languageeng
creationdate2010
topic
0Human Embryonic Stem Cells
1Chondrogenic
2Cartilage
3Differentiation
4Hyaluronan
5Hydrogel
collectionScienceDirect Journals (Elsevier)
prefilterarticles
rsrctypearticles
creatorcontrib
0Toh, Wei Seong
1Lee, Eng Hin
2Guo, Xi-Min
3Chan, Jerry K.Y.
4Yeow, Chen Hua
5Choo, Andre B.
6Cao, Tong
jtitleBiomaterials
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Toh
1Lee
2Guo
3Chan
4Yeow
5Choo
6Cao
aufirstWei Seong
au
0Toh, Wei Seong
1Lee, Eng Hin
2Guo, Xi-Min
3Chan, Jerry K.Y.
4Yeow, Chen Hua
5Choo, Andre B.
6Cao, Tong
atitleCartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells
jtitleBiomaterials
date2010
risdate2010
volume31
issue27
spage6968
epage6980
pages6968-6980
issn0142-9612
formatjournal
genrearticle
ristypeJOUR
abstractHuman embryonic stem cells (hESCs) have the potential to offer a virtually unlimited source of chondrogenic cells for use in cartilage repair and regeneration. We have recently shown that expandable chondrogenic cells can be derived from hESCs under selective growth factor-responsive conditions. In this study, we explore the potential of these hESC-derived chondrogenic cells to produce an extracellular matrix (ECM)-enriched cartilaginous tissue construct when cultured in hyaluronic acid (HA)-based hydrogel, and further investigated the long-term reparative ability of the resulting hESC-derived chondrogenic cell-engineered cartilage (HCCEC) in an osteochondral defect model. We hypothesized that HCCEC can provide a functional template capable of undergoing orderly remodeling during the repair of critical-sized osteochondral defects (1.5 mm in diameter, 1 mm depth into the subchondral bone) in a rat model. In the process of repair, we observed an orderly spatial-temporal remodeling of HCCEC over 12 weeks into osteochondral tissue, with characteristic architectural features including a hyaline-like neocartilage layer with good surface regularity and complete integration with the adjacent host cartilage and a regenerated subchondral bone. By 12 weeks, the HCCEC-regenerated osteochondral tissue resembled closely that of age-matched unoperated native control, while only fibrous tissue filled in the control defects which left empty or treated with hydrogel alone. Here we demonstrate that transplanted hESC-derived chondrogenic cells maintain long-term viability with no evidence of tumorigenicity, providing a safe, highly-efficient and practical strategy of applying hESCs for cartilage tissue engineering.
pubElsevier Ltd
doi10.1016/j.biomaterials.2010.05.064
eissn18785905