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A new finding concerning adenoviral-mediated gene transfer: A high-level, cell-specific transgene expression in the neural stem cells of adult mice

Graphical abstractPattern of EGFP expression on a 35μm section of the hippocampus at different time points after intracerebral injection of Ad-mCMV-EGFP (1×108iu). Highlights► We transferred Ad-mCMV-EGFP to the hippocampus and the LV of adult mice brain. ► The neural stem cells in the dentate SGZ an... Full description

Journal Title: Journal of Virological Methods December 2012, Vol.186(1-2), pp.1-6
Main Author: Yan, Rong
Other Authors: Zhang, Lin , Zhang, Qi , Li, Jia , Kang, Xiaokui , Wang, Haining , Zhang, Jianning , Yang, Shuyuan , Yang, Xinyu
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0166-0934 ; DOI: 10.1016/j.jviromet.2012.07.012
Link: http://dx.doi.org/10.1016/j.jviromet.2012.07.012
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recordid: sciversesciencedirect_elsevierS0166-0934(12)00253-4
title: A new finding concerning adenoviral-mediated gene transfer: A high-level, cell-specific transgene expression in the neural stem cells of adult mice
format: Article
creator:
  • Yan, Rong
  • Zhang, Lin
  • Zhang, Qi
  • Li, Jia
  • Kang, Xiaokui
  • Wang, Haining
  • Zhang, Jianning
  • Yang, Shuyuan
  • Yang, Xinyu
subjects:
  • Ad-Mcmv
  • Gene Transfer
  • Dentate Gyrus
  • Subgranular Zone
  • Neural Stem Cell
ispartof: Journal of Virological Methods, December 2012, Vol.186(1-2), pp.1-6
description: Graphical abstractPattern of EGFP expression on a 35μm section of the hippocampus at different time points after intracerebral injection of Ad-mCMV-EGFP (1×108iu). Highlights► We transferred Ad-mCMV-EGFP to the hippocampus and the LV of adult mice brain. ► The neural stem cells in the dentate SGZ and SVZ were preferentially transduced. ► Minimal neurons were transduced in the granule cell layer of the dentate gyrus. ► No EGFP was detected in the pyramidal cell layers of CAl to CA3 area. ► EGFP activity can be detected for 2 months after infection. Adenoviruses are highly efficient vectors for gene transfer into brain cells. Restricting transgene expression to specific cell types and maintaining long-term expression are major goals for adenoviral-mediated gene transfer in the central nervous system. Human adenovirus type 5 (Ad5) mediated transgene expression is described under the control of the murine cytomegalovirus (mCMV) immediate-early promoter. It was found that the neural stem cells in the dentate subgranular zone were transduced preferentially, minimal neurons were transduced in the granule cell layer of the dentate gyrus, no EGFP was detected in the pyramidal cell layers of CAl to CA3 area and EGFP activity can be detected for 2 months after infection. Therefore, the mCMV-adenoviral vectors can be used both for studying the function of various genes in the differentiation of neural stem cells and, ultimately, for gene therapy or to modulate specific gene expression.
language: eng
source:
identifier: ISSN: 0166-0934 ; DOI: 10.1016/j.jviromet.2012.07.012
fulltext: fulltext
issn:
  • 01660934
  • 0166-0934
url: Link


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titleA new finding concerning adenoviral-mediated gene transfer: A high-level, cell-specific transgene expression in the neural stem cells of adult mice
creatorYan, Rong ; Zhang, Lin ; Zhang, Qi ; Li, Jia ; Kang, Xiaokui ; Wang, Haining ; Zhang, Jianning ; Yang, Shuyuan ; Yang, Xinyu
ispartofJournal of Virological Methods, December 2012, Vol.186(1-2), pp.1-6
identifierISSN: 0166-0934 ; DOI: 10.1016/j.jviromet.2012.07.012
subjectAd-Mcmv ; Gene Transfer ; Dentate Gyrus ; Subgranular Zone ; Neural Stem Cell
descriptionGraphical abstractPattern of EGFP expression on a 35μm section of the hippocampus at different time points after intracerebral injection of Ad-mCMV-EGFP (1×108iu). Highlights► We transferred Ad-mCMV-EGFP to the hippocampus and the LV of adult mice brain. ► The neural stem cells in the dentate SGZ and SVZ were preferentially transduced. ► Minimal neurons were transduced in the granule cell layer of the dentate gyrus. ► No EGFP was detected in the pyramidal cell layers of CAl to CA3 area. ► EGFP activity can be detected for 2 months after infection. Adenoviruses are highly efficient vectors for gene transfer into brain cells. Restricting transgene expression to specific cell types and maintaining long-term expression are major goals for adenoviral-mediated gene transfer in the central nervous system. Human adenovirus type 5 (Ad5) mediated transgene expression is described under the control of the murine cytomegalovirus (mCMV) immediate-early promoter. It was found that the neural stem cells in the dentate subgranular zone were transduced preferentially, minimal neurons were transduced in the granule cell layer of the dentate gyrus, no EGFP was detected in the pyramidal cell layers of CAl to CA3 area and EGFP activity can be detected for 2 months after infection. Therefore, the mCMV-adenoviral vectors can be used both for studying the function of various genes in the differentiation of neural stem cells and, ultimately, for gene therapy or to modulate specific gene expression.
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abstractGraphical abstractPattern of EGFP expression on a 35μm section of the hippocampus at different time points after intracerebral injection of Ad-mCMV-EGFP (1×108iu). Highlights► We transferred Ad-mCMV-EGFP to the hippocampus and the LV of adult mice brain. ► The neural stem cells in the dentate SGZ and SVZ were preferentially transduced. ► Minimal neurons were transduced in the granule cell layer of the dentate gyrus. ► No EGFP was detected in the pyramidal cell layers of CAl to CA3 area. ► EGFP activity can be detected for 2 months after infection. Adenoviruses are highly efficient vectors for gene transfer into brain cells. Restricting transgene expression to specific cell types and maintaining long-term expression are major goals for adenoviral-mediated gene transfer in the central nervous system. Human adenovirus type 5 (Ad5) mediated transgene expression is described under the control of the murine cytomegalovirus (mCMV) immediate-early promoter. It was found that the neural stem cells in the dentate subgranular zone were transduced preferentially, minimal neurons were transduced in the granule cell layer of the dentate gyrus, no EGFP was detected in the pyramidal cell layers of CAl to CA3 area and EGFP activity can be detected for 2 months after infection. Therefore, the mCMV-adenoviral vectors can be used both for studying the function of various genes in the differentiation of neural stem cells and, ultimately, for gene therapy or to modulate specific gene expression.
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