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Visualization of mouse barrel cortex using ex-vivo track density imaging

We describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adult mouse brain with short-tracks track density imaging (stTDI). stTDI produced much higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color... Full description

Journal Title: NeuroImage 15 February 2014, Vol.87, pp.465-475
Main Author: Kurniawan, Nyoman D.
Other Authors: Richards, Kay L. , Yang, Zhengyi , She, David , Ullmann, Jeremy F.P. , Moldrich, Randal X. , Liu, Sha , Yaksic, Javier Urriola , Leanage, Gayeshika , Kharatishvili, Irina , Wimmer, Verena , Calamante, Fernando , Galloway, Graham J. , Petrou, Steven , Reutens, David C.
Format: Electronic Article Electronic Article
Language: English
Subjects:
Csd
Dec
Dti
Dwi
Fod
FA
Sh
Tdi
Vpm
ID: ISSN: 1053-8119 ; DOI: 10.1016/j.neuroimage.2013.09.030
Link: http://dx.doi.org/10.1016/j.neuroimage.2013.09.030
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recordid: sciversesciencedirect_elsevierS1053-8119(13)00966-X
title: Visualization of mouse barrel cortex using ex-vivo track density imaging
format: Article
creator:
  • Kurniawan, Nyoman D.
  • Richards, Kay L.
  • Yang, Zhengyi
  • She, David
  • Ullmann, Jeremy F.P.
  • Moldrich, Randal X.
  • Liu, Sha
  • Yaksic, Javier Urriola
  • Leanage, Gayeshika
  • Kharatishvili, Irina
  • Wimmer, Verena
  • Calamante, Fernando
  • Galloway, Graham J.
  • Petrou, Steven
  • Reutens, David C.
subjects:
  • Csd
  • Dec
  • Dti
  • Dwi
  • Fod
  • FA
  • Hardi
  • Sh
  • Sttdi
  • Tdi
  • Vpm
  • Pmbsf
  • Mouse Brain
  • Barrel Cortex
  • Magnetic Resonance
  • Diffusion Weighted Imaging
  • Track-Density Imaging
  • Infraorbital Nerve Cut
ispartof: NeuroImage, 15 February 2014, Vol.87, pp.465-475
description: We describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adult mouse brain with short-tracks track density imaging (stTDI). stTDI produced much higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color FA maps, spin-echo T1- and T2-weighted imaging and gradient echo T1/T2*-weighted imaging. 3D high angular resolution diffusion imaging (HARDI) data were acquired at 48micron isotropic resolution for a (3mm)3 block of cortex containing the barrel field and reconstructed using stTDI at 10micron isotropic resolution. HARDI data were also acquired at 100micron isotropic resolution to image the whole brain and reconstructed using stTDI at 20micron isotropic resolution. The 10micron resolution stTDI maps showed exceptionally clear delineation of barrel structures. Individual barrels could also be distinguished in the 20micron stTDI maps but the septa separating the individual barrels appeared thicker compared to the 10micron maps, indicating that the ability of stTDI to produce high quality structural delineation is dependent upon acquisition resolution. Close homology was observed between the barrel structure delineated using stTDI and reconstructed histological data from the same samples. stTDI also detects barrel deletions in the posterior medial barrel sub-field in mice with infraorbital nerve cuts. The results demonstrate that stTDI is a novel imaging technique that enables three-dimensional characterization of complex structures such as the barrels in S1 and provides an important complementary non-invasive imaging tool for studying synaptic connectivity, development and plasticity of the sensory system. •Mouse barrel cortex can be visualized in high definition using stTDI.•stTDI produced superior barrel visualization compared to conventional MRI and FA maps.•stTDI is a novel imaging technique for 3D characterization of the barrel cortex.•stTDI can detect barrel changes resulting from infraorbital nerve cut.
language: eng
source:
identifier: ISSN: 1053-8119 ; DOI: 10.1016/j.neuroimage.2013.09.030
fulltext: fulltext
issn:
  • 10538119
  • 1053-8119
url: Link


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titleVisualization of mouse barrel cortex using ex-vivo track density imaging
creatorKurniawan, Nyoman D. ; Richards, Kay L. ; Yang, Zhengyi ; She, David ; Ullmann, Jeremy F.P. ; Moldrich, Randal X. ; Liu, Sha ; Yaksic, Javier Urriola ; Leanage, Gayeshika ; Kharatishvili, Irina ; Wimmer, Verena ; Calamante, Fernando ; Galloway, Graham J. ; Petrou, Steven ; Reutens, David C.
ispartofNeuroImage, 15 February 2014, Vol.87, pp.465-475
identifierISSN: 1053-8119 ; DOI: 10.1016/j.neuroimage.2013.09.030
subjectCsd ; Dec ; Dti ; Dwi ; Fod ; FA ; Hardi ; Sh ; Sttdi ; Tdi ; Vpm ; Pmbsf ; Mouse Brain ; Barrel Cortex ; Magnetic Resonance ; Diffusion Weighted Imaging ; Track-Density Imaging ; Infraorbital Nerve Cut
descriptionWe describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adult mouse brain with short-tracks track density imaging (stTDI). stTDI produced much higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color FA maps, spin-echo T1- and T2-weighted imaging and gradient echo T1/T2*-weighted imaging. 3D high angular resolution diffusion imaging (HARDI) data were acquired at 48micron isotropic resolution for a (3mm)3 block of cortex containing the barrel field and reconstructed using stTDI at 10micron isotropic resolution. HARDI data were also acquired at 100micron isotropic resolution to image the whole brain and reconstructed using stTDI at 20micron isotropic resolution. The 10micron resolution stTDI maps showed exceptionally clear delineation of barrel structures. Individual barrels could also be distinguished in the 20micron stTDI maps but the septa separating the individual barrels appeared thicker compared to the 10micron maps, indicating that the ability of stTDI to produce high quality structural delineation is dependent upon acquisition resolution. Close homology was observed between the barrel structure delineated using stTDI and reconstructed histological data from the same samples. stTDI also detects barrel deletions in the posterior medial barrel sub-field in mice with infraorbital nerve cuts. The results demonstrate that stTDI is a novel imaging technique that enables three-dimensional characterization of complex structures such as the barrels in S1 and provides an important complementary non-invasive imaging tool for studying synaptic connectivity, development and plasticity of the sensory system. •Mouse barrel cortex can be visualized in high definition using stTDI.•stTDI produced superior barrel visualization compared to conventional MRI and FA maps.•stTDI is a novel imaging technique for 3D characterization of the barrel cortex.•stTDI can detect barrel changes resulting from infraorbital nerve cut.
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titleVisualization of mouse barrel cortex using ex-vivo track density imaging
descriptionWe describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adult mouse brain with short-tracks track density imaging (stTDI). stTDI produced much higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color FA maps, spin-echo T1- and T2-weighted imaging and gradient echo T1/T2*-weighted imaging. 3D high angular resolution diffusion imaging (HARDI) data were acquired at 48micron isotropic resolution for a (3mm)3 block of cortex containing the barrel field and reconstructed using stTDI at 10micron isotropic resolution. HARDI data were also acquired at 100micron isotropic resolution to image the whole brain and reconstructed using stTDI at 20micron isotropic resolution. The 10micron resolution stTDI maps showed exceptionally clear delineation of barrel structures. Individual barrels could also be distinguished in the 20micron stTDI maps but the septa separating the individual barrels appeared thicker compared to the 10micron maps, indicating that the ability of stTDI to produce high quality structural delineation is dependent upon acquisition resolution. Close homology was observed between the barrel structure delineated using stTDI and reconstructed histological data from the same samples. stTDI also detects barrel deletions in the posterior medial barrel sub-field in mice with infraorbital nerve cuts. The results demonstrate that stTDI is a novel imaging technique that enables three-dimensional characterization of complex structures such as the barrels in S1 and provides an important complementary non-invasive imaging tool for studying synaptic connectivity, development and plasticity of the sensory system. •Mouse barrel cortex can be visualized in high definition using stTDI.•stTDI produced superior barrel visualization compared to conventional MRI and FA maps.•stTDI is a novel imaging technique for 3D characterization of the barrel cortex.•stTDI can detect barrel changes resulting from infraorbital nerve cut.
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authorKurniawan, Nyoman D. ; Richards, Kay L. ; Yang, Zhengyi ; She, David ; Ullmann, Jeremy F.P. ; Moldrich, Randal X. ; Liu, Sha ; Yaksic, Javier Urriola ; Leanage, Gayeshika ; Kharatishvili, Irina ; Wimmer, Verena ; Calamante, Fernando ; Galloway, Graham J. ; Petrou, Steven ; Reutens, David C.
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abstractWe describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adult mouse brain with short-tracks track density imaging (stTDI). stTDI produced much higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color FA maps, spin-echo T1- and T2-weighted imaging and gradient echo T1/T2*-weighted imaging. 3D high angular resolution diffusion imaging (HARDI) data were acquired at 48micron isotropic resolution for a (3mm)3 block of cortex containing the barrel field and reconstructed using stTDI at 10micron isotropic resolution. HARDI data were also acquired at 100micron isotropic resolution to image the whole brain and reconstructed using stTDI at 20micron isotropic resolution. The 10micron resolution stTDI maps showed exceptionally clear delineation of barrel structures. Individual barrels could also be distinguished in the 20micron stTDI maps but the septa separating the individual barrels appeared thicker compared to the 10micron maps, indicating that the ability of stTDI to produce high quality structural delineation is dependent upon acquisition resolution. Close homology was observed between the barrel structure delineated using stTDI and reconstructed histological data from the same samples. stTDI also detects barrel deletions in the posterior medial barrel sub-field in mice with infraorbital nerve cuts. The results demonstrate that stTDI is a novel imaging technique that enables three-dimensional characterization of complex structures such as the barrels in S1 and provides an important complementary non-invasive imaging tool for studying synaptic connectivity, development and plasticity of the sensory system. •Mouse barrel cortex can be visualized in high definition using stTDI.•stTDI produced superior barrel visualization compared to conventional MRI and FA maps.•stTDI is a novel imaging technique for 3D characterization of the barrel cortex.•stTDI can detect barrel changes resulting from infraorbital nerve cut.
pubElsevier Inc.
doi10.1016/j.neuroimage.2013.09.030
eissn10959572
date2014-02-15