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Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging

In this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20μm isotropic... Full description

Journal Title: NeuroImage 15 November 2014, Vol.102, pp.381-392
Main Author: Richards, Kay
Other Authors: Calamante, Fernando , Tournier, Jacques-Donald , Kurniawan, Nyoman D. , Sadeghian, Farnoosh , Retchford, Alexander R. , Jones, Gabriel Davis , Reid, Christopher A. , Reutens, David C. , Ordidge, Roger , Connelly, Alan , Petrou, Steven
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1053-8119 ; DOI: 10.1016/j.neuroimage.2014.07.048
Link: http://dx.doi.org/10.1016/j.neuroimage.2014.07.048
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recordid: sciversesciencedirect_elsevierS1053-8119(14)00631-4
title: Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging
format: Article
creator:
  • Richards, Kay
  • Calamante, Fernando
  • Tournier, Jacques-Donald
  • Kurniawan, Nyoman D.
  • Sadeghian, Farnoosh
  • Retchford, Alexander R.
  • Jones, Gabriel Davis
  • Reid, Christopher A.
  • Reutens, David C.
  • Ordidge, Roger
  • Connelly, Alan
  • Petrou, Steven
subjects:
  • Somatosensory Pathway
  • Fiber Tracking
  • Connectivity
  • Mouse Brain
  • Track-Density Imaging
ispartof: NeuroImage, 15 November 2014, Vol.102, pp.381-392
description: In this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant with results obtained using anterograde dye tracing from brainstem to thalamus. Importantly, TDI mapping overcame the constraint of tissue distortion observed in mechanically sectioned tissue, enabling 3D reconstruction and long-range connectivity data. In conclusion, our results showed that diffusion micro-imaging at ultra-high field MRI revealed the stereotypical pattern of somatosensory connectivity and is a valuable tool to complement histologic methods, achieving 3D spatial preservation of whole brain networks for characterization in mouse models of human disease. •Stereotypical pattern of somatosensory relay locations revealed by TDI mapping•Long-range pathways inferred by TDI were consistent with fiber-tracing methods.•Structure and connectivity of TDI mapping approaching histology level
language: eng
source:
identifier: ISSN: 1053-8119 ; DOI: 10.1016/j.neuroimage.2014.07.048
fulltext: fulltext
issn:
  • 10538119
  • 1053-8119
url: Link


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titleMapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging
creatorRichards, Kay ; Calamante, Fernando ; Tournier, Jacques-Donald ; Kurniawan, Nyoman D. ; Sadeghian, Farnoosh ; Retchford, Alexander R. ; Jones, Gabriel Davis ; Reid, Christopher A. ; Reutens, David C. ; Ordidge, Roger ; Connelly, Alan ; Petrou, Steven
ispartofNeuroImage, 15 November 2014, Vol.102, pp.381-392
identifierISSN: 1053-8119 ; DOI: 10.1016/j.neuroimage.2014.07.048
subjectSomatosensory Pathway ; Fiber Tracking ; Connectivity ; Mouse Brain ; Track-Density Imaging
descriptionIn this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant with results obtained using anterograde dye tracing from brainstem to thalamus. Importantly, TDI mapping overcame the constraint of tissue distortion observed in mechanically sectioned tissue, enabling 3D reconstruction and long-range connectivity data. In conclusion, our results showed that diffusion micro-imaging at ultra-high field MRI revealed the stereotypical pattern of somatosensory connectivity and is a valuable tool to complement histologic methods, achieving 3D spatial preservation of whole brain networks for characterization in mouse models of human disease. •Stereotypical pattern of somatosensory relay locations revealed by TDI mapping•Long-range pathways inferred by TDI were consistent with fiber-tracing methods.•Structure and connectivity of TDI mapping approaching histology level
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abstractIn this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant with results obtained using anterograde dye tracing from brainstem to thalamus. Importantly, TDI mapping overcame the constraint of tissue distortion observed in mechanically sectioned tissue, enabling 3D reconstruction and long-range connectivity data. In conclusion, our results showed that diffusion micro-imaging at ultra-high field MRI revealed the stereotypical pattern of somatosensory connectivity and is a valuable tool to complement histologic methods, achieving 3D spatial preservation of whole brain networks for characterization in mouse models of human disease. •Stereotypical pattern of somatosensory relay locations revealed by TDI mapping•Long-range pathways inferred by TDI were consistent with fiber-tracing methods.•Structure and connectivity of TDI mapping approaching histology level
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