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Effects of arsenic sulfide (As 2 S 2 ) on B and T lymphoma cell lines and possible underlying mechanisms

Lymphoma is a hematological malignancy that originates from lymph nodes and lymphoid tissues and is divided into Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL), based on its histopathological characteristics. The aim of this study was to investigate the effects of arsenic sulfide (As 2 S 2 ),... Full description

Journal Title: Biomedical Reports 07/2013, Vol.1(4), pp.583-588
Main Author: Li, Xianglu
Other Authors: Liu, Xinyu , Wang, Ling , Lv, Xiao , Li, Peipei , Lu, Kang , Wang, Xin
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 2049-9434 ; E-ISSN: 2049-9442 ; DOI: 10.3892/br.2013.119
Link: http://www.spandidos-publications.com/br/1/4/583
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recordid: spandido10.3892/br.2013.119
title: Effects of arsenic sulfide (As 2 S 2 ) on B and T lymphoma cell lines and possible underlying mechanisms
format: Article
creator:
  • Li, Xianglu
  • Liu, Xinyu
  • Wang, Ling
  • Lv, Xiao
  • Li, Peipei
  • Lu, Kang
  • Wang, Xin
subjects:
  • Arsenic Sulfide
  • Raji
  • Jurkat
  • Proliferation
  • Apoptosis
  • Genes
ispartof: Biomedical Reports, 07/2013, Vol.1(4), pp.583-588
description: Lymphoma is a hematological malignancy that originates from lymph nodes and lymphoid tissues and is divided into Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL), based on its histopathological characteristics. The aim of this study was to investigate the effects of arsenic sulfide (As 2 S 2 ), the main ingredient of realgar, on the proliferation and apoptosis of the Raji B-cell lymphoma and Jurkat T-cell lymphoma lines, comparing the sensitivity between the two cell lines and investigating the possible underlying mechanisms. The two lymphoma cell lines were cultured in vitro , using different concentrations of As 2 S 2 for different time periods. The cell proliferation was detected using the Cell Counting kit-8 (CCK-8). Apoptosis was assessed via flow cytometry. Expression levels of the apoptosis-associated genes [ Homo sapiens Bcl-2-associated X protein (BAX), Homo sapiens B-cell CLL/lymphoma 2 (Bcl-2), Homo sapiens Bcl-2-like protein 1 (BCL2L1, Bcl-xL), Homo sapiens v-myc myelocytomatosis viral oncogene homolog (avian) (MYC, c-Myc) and Homo sapiens pim-1 oncogene (PIM)] were measured via the reverse transcription polymerase chain reaction (RT-PCR) method. The results demonstrated that As 2 S 2 inhibited proliferation and induced apoptosis in the two lymphoma cell lines in a time- and concentration-dependent manner, with the Raji cells being more sensitive to As 2 S 2 compared to Jurkat cells. As 2 S 2 may also alter the expression levels of different apoptosis-associated genes, with the alterations of the mRNA expression levels being different between Raji and Jurkat cells. These findings indicated that As 2 S 2 may inhibit the proliferation and promote the apoptosis of non-Hodgkin lymphoma (NHL) cell lines and that B-cell lymphoma cell lines are more sensitive compared to T-cell lymphoma cell lines. The possible underlying mechanism is that As 2 S 2 alters the expression levels of the apoptosis-associated genes and activates apoptosis-associated signaling pathways.
language: eng
source:
identifier: ISSN: 2049-9434 ; E-ISSN: 2049-9442 ; DOI: 10.3892/br.2013.119
fulltext: fulltext
issn:
  • 2049-9434
  • 20499434
  • 2049-9442
  • 20499442
url: Link


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titleEffects of arsenic sulfide (As 2 S 2 ) on B and T lymphoma cell lines and possible underlying mechanisms
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ispartofBiomedical Reports, 07/2013, Vol.1(4), pp.583-588
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subjectArsenic Sulfide ; Raji ; Jurkat ; Proliferation ; Apoptosis ; Genes
descriptionLymphoma is a hematological malignancy that originates from lymph nodes and lymphoid tissues and is divided into Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL), based on its histopathological characteristics. The aim of this study was to investigate the effects of arsenic sulfide (As 2 S 2 ), the main ingredient of realgar, on the proliferation and apoptosis of the Raji B-cell lymphoma and Jurkat T-cell lymphoma lines, comparing the sensitivity between the two cell lines and investigating the possible underlying mechanisms. The two lymphoma cell lines were cultured in vitro , using different concentrations of As 2 S 2 for different time periods. The cell proliferation was detected using the Cell Counting kit-8 (CCK-8). Apoptosis was assessed via flow cytometry. Expression levels of the apoptosis-associated genes [ Homo sapiens Bcl-2-associated X protein (BAX), Homo sapiens B-cell CLL/lymphoma 2 (Bcl-2), Homo sapiens Bcl-2-like protein 1 (BCL2L1, Bcl-xL), Homo sapiens v-myc myelocytomatosis viral oncogene homolog (avian) (MYC, c-Myc) and Homo sapiens pim-1 oncogene (PIM)] were measured via the reverse transcription polymerase chain reaction (RT-PCR) method. The results demonstrated that As 2 S 2 inhibited proliferation and induced apoptosis in the two lymphoma cell lines in a time- and concentration-dependent manner, with the Raji cells being more sensitive to As 2 S 2 compared to Jurkat cells. As 2 S 2 may also alter the expression levels of different apoptosis-associated genes, with the alterations of the mRNA expression levels being different between Raji and Jurkat cells. These findings indicated that As 2 S 2 may inhibit the proliferation and promote the apoptosis of non-Hodgkin lymphoma (NHL) cell lines and that B-cell lymphoma cell lines are more sensitive compared to T-cell lymphoma cell lines. The possible underlying mechanism is that As 2 S 2 alters the expression levels of the apoptosis-associated genes and activates apoptosis-associated signaling pathways.
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titleEffects of arsenic sulfide (As 2 S 2 ) on B and T lymphoma cell lines and possible underlying mechanisms
descriptionLymphoma is a hematological malignancy that originates from lymph nodes and lymphoid tissues and is divided into Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL), based on its histopathological characteristics. The aim of this study was to investigate the effects of arsenic sulfide (As 2 S 2 ), the main ingredient of realgar, on the proliferation and apoptosis of the Raji B-cell lymphoma and Jurkat T-cell lymphoma lines, comparing the sensitivity between the two cell lines and investigating the possible underlying mechanisms. The two lymphoma cell lines were cultured in vitro , using different concentrations of As 2 S 2 for different time periods. The cell proliferation was detected using the Cell Counting kit-8 (CCK-8). Apoptosis was assessed via flow cytometry. Expression levels of the apoptosis-associated genes [ Homo sapiens Bcl-2-associated X protein (BAX), Homo sapiens B-cell CLL/lymphoma 2 (Bcl-2), Homo sapiens Bcl-2-like protein 1 (BCL2L1, Bcl-xL), Homo sapiens v-myc myelocytomatosis viral oncogene homolog (avian) (MYC, c-Myc) and Homo sapiens pim-1 oncogene (PIM)] were measured via the reverse transcription polymerase chain reaction (RT-PCR) method. The results demonstrated that As 2 S 2 inhibited proliferation and induced apoptosis in the two lymphoma cell lines in a time- and concentration-dependent manner, with the Raji cells being more sensitive to As 2 S 2 compared to Jurkat cells. As 2 S 2 may also alter the expression levels of different apoptosis-associated genes, with the alterations of the mRNA expression levels being different between Raji and Jurkat cells. These findings indicated that As 2 S 2 may inhibit the proliferation and promote the apoptosis of non-Hodgkin lymphoma (NHL) cell lines and that B-cell lymphoma cell lines are more sensitive compared to T-cell lymphoma cell lines. The possible underlying mechanism is that As 2 S 2 alters the expression levels of the apoptosis-associated genes and activates apoptosis-associated signaling pathways.
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abstractLymphoma is a hematological malignancy that originates from lymph nodes and lymphoid tissues and is divided into Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL), based on its histopathological characteristics. The aim of this study was to investigate the effects of arsenic sulfide (As 2 S 2 ), the main ingredient of realgar, on the proliferation and apoptosis of the Raji B-cell lymphoma and Jurkat T-cell lymphoma lines, comparing the sensitivity between the two cell lines and investigating the possible underlying mechanisms. The two lymphoma cell lines were cultured in vitro , using different concentrations of As 2 S 2 for different time periods. The cell proliferation was detected using the Cell Counting kit-8 (CCK-8). Apoptosis was assessed via flow cytometry. Expression levels of the apoptosis-associated genes [ Homo sapiens Bcl-2-associated X protein (BAX), Homo sapiens B-cell CLL/lymphoma 2 (Bcl-2), Homo sapiens Bcl-2-like protein 1 (BCL2L1, Bcl-xL), Homo sapiens v-myc myelocytomatosis viral oncogene homolog (avian) (MYC, c-Myc) and Homo sapiens pim-1 oncogene (PIM)] were measured via the reverse transcription polymerase chain reaction (RT-PCR) method. The results demonstrated that As 2 S 2 inhibited proliferation and induced apoptosis in the two lymphoma cell lines in a time- and concentration-dependent manner, with the Raji cells being more sensitive to As 2 S 2 compared to Jurkat cells. As 2 S 2 may also alter the expression levels of different apoptosis-associated genes, with the alterations of the mRNA expression levels being different between Raji and Jurkat cells. These findings indicated that As 2 S 2 may inhibit the proliferation and promote the apoptosis of non-Hodgkin lymphoma (NHL) cell lines and that B-cell lymphoma cell lines are more sensitive compared to T-cell lymphoma cell lines. The possible underlying mechanism is that As 2 S 2 alters the expression levels of the apoptosis-associated genes and activates apoptosis-associated signaling pathways.
pubD.A. Spandidos
doi10.3892/br.2013.119
date2013-07