schliessen

Filtern

 

Bibliotheken

MicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer

MicroRNA-17 (miRNA-17/miR-17) expression has been confirmed to be significantly higher in colorectal cancer tissues than in normal tissues. However, its exact role in colorectal cancer has not yet been fully elucidated. In this study, we found that miR-17 not only promoted epithelial-mesenchymal tra... Full description

Journal Title: International Journal of Molecular Medicine 08/2016, Vol.38(2), pp.499-506
Main Author: Xi, Xiang-Peng
Other Authors: Zhuang, Jing , Teng, Mu-Jian , Xia, Li-Jian , Yang, Ming-Yu , Liu, Qing-Gen , Chen, Jing-Bo
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1107-3756 ; E-ISSN: 1791-244X ; DOI: 10.3892/ijmm.2016.2624
Link: http://www.spandidos-publications.com/ijmm/38/2/499
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: spandido10.3892/ijmm.2016.2624
title: MicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer
format: Article
creator:
  • Xi, Xiang-Peng
  • Zhuang, Jing
  • Teng, Mu-Jian
  • Xia, Li-Jian
  • Yang, Ming-Yu
  • Liu, Qing-Gen
  • Chen, Jing-Bo
subjects:
  • Microrna-17
  • Epithelial-Mesenchymal Transition
  • Cyp7b1
  • Colorectal Cancer
ispartof: International Journal of Molecular Medicine, 08/2016, Vol.38(2), pp.499-506
description: MicroRNA-17 (miRNA-17/miR-17) expression has been confirmed to be significantly higher in colorectal cancer tissues than in normal tissues. However, its exact role in colorectal cancer has not yet been fully elucidated. In this study, we found that miR-17 not only promoted epithelial-mesenchymal transition (EMT), but also promoted the formation of a stem cell-like population in colon cancer DLD1 cells. We also wished to determine the role of cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) in CRC. miR-17 was overexpressed using a recombinant plasmid and CYP7B1 was silenced by transfection with shRNA. Western blot analysis was used to determine protein expression in the DLD1 cells and in tumor tissues obtained from patients with colon cancer. Our results revealed that miR-17 overexpression led to the degradation of CYP7B1 mRNA expression in DLD1 cells. In addition, we found that the silencing of CYB7B1 promoted EMT and the formation of a stem cell-like population in the cells. Thus, our findings demonstrate that miR-17 induces EMT consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer.
language: eng
source:
identifier: ISSN: 1107-3756 ; E-ISSN: 1791-244X ; DOI: 10.3892/ijmm.2016.2624
fulltext: fulltext
issn:
  • 1107-3756
  • 11073756
  • 1791-244X
  • 1791244X
url: Link


@attributes
ID534202849
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.3892/ijmm.2016.2624
sourceidspandido
recordidTN_spandido10.3892/ijmm.2016.2624
sourceformatXML
sourcesystemPC
pqid1805759726
galeid459658204
display
typearticle
titleMicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer
creatorXi, Xiang-Peng ; Zhuang, Jing ; Teng, Mu-Jian ; Xia, Li-Jian ; Yang, Ming-Yu ; Liu, Qing-Gen ; Chen, Jing-Bo
ispartofInternational Journal of Molecular Medicine, 08/2016, Vol.38(2), pp.499-506
identifier
subjectMicrorna-17 ; Epithelial-Mesenchymal Transition ; Cyp7b1 ; Colorectal Cancer
descriptionMicroRNA-17 (miRNA-17/miR-17) expression has been confirmed to be significantly higher in colorectal cancer tissues than in normal tissues. However, its exact role in colorectal cancer has not yet been fully elucidated. In this study, we found that miR-17 not only promoted epithelial-mesenchymal transition (EMT), but also promoted the formation of a stem cell-like population in colon cancer DLD1 cells. We also wished to determine the role of cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) in CRC. miR-17 was overexpressed using a recombinant plasmid and CYP7B1 was silenced by transfection with shRNA. Western blot analysis was used to determine protein expression in the DLD1 cells and in tumor tissues obtained from patients with colon cancer. Our results revealed that miR-17 overexpression led to the degradation of CYP7B1 mRNA expression in DLD1 cells. In addition, we found that the silencing of CYB7B1 promoted EMT and the formation of a stem cell-like population in the cells. Thus, our findings demonstrate that miR-17 induces EMT consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer.
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://www.spandidos-publications.com/ijmm/38/2/499$$EView_this_record_in_Spandidos_Publications
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Xi, Xiang-Peng
1Zhuang, Jing
2Teng, Mu-Jian
3Xia, Li-Jian
4Yang, Ming-Yu
5Liu, Qing-Gen
6Chen, Jing-Bo
titleMicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer
descriptionMicroRNA-17 (miRNA-17/miR-17) expression has been confirmed to be significantly higher in colorectal cancer tissues than in normal tissues. However, its exact role in colorectal cancer has not yet been fully elucidated. In this study, we found that miR-17 not only promoted epithelial-mesenchymal transition (EMT), but also promoted the formation of a stem cell-like population in colon cancer DLD1 cells. We also wished to determine the role of cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) in CRC. miR-17 was overexpressed using a recombinant plasmid and CYP7B1 was silenced by transfection with shRNA. Western blot analysis was used to determine protein expression in the DLD1 cells and in tumor tissues obtained from patients with colon cancer. Our results revealed that miR-17 overexpression led to the degradation of CYP7B1 mRNA expression in DLD1 cells. In addition, we found that the silencing of CYB7B1 promoted EMT and the formation of a stem cell-like population in the cells. Thus, our findings demonstrate that miR-17 induces EMT consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer.
subject
0microRNA-17
1epithelial-mesenchymal transition
2CYP7B1
3colorectal cancer
general
010.3892/ijmm.2016.2624
1D.A. Spandidos
2English
3Spandidios Publications (Spandidos Publications Ltd.)
sourceidspandido
recordidspandido10.3892/ijmm.2016.2624
issn
01107-3756
111073756
21791-244X
31791244X
rsrctypearticle
creationdate2016
addtitleInternational Journal of Molecular Medicine
searchscopespandidos
scopespandidos
lsr30VSR-Enriched:[pqid, galeid]
sort
titleMicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer
authorXi, Xiang-Peng ; Zhuang, Jing ; Teng, Mu-Jian ; Xia, Li-Jian ; Yang, Ming-Yu ; Liu, Qing-Gen ; Chen, Jing-Bo
creationdate20160800
lso0120160800
facets
frbrgroupid7608834896196677499
frbrtype5
languageeng
creationdate2016
topic
0microRNA-17
1epithelial-mesenchymal transition
2CYP7B1
3colorectal cancer
collectionSpandidios Publications (Spandidos Publications Ltd.)
prefilterarticles
rsrctypearticles
creatorcontrib
0Xi, Xiang-Peng
1Zhuang, Jing
2Teng, Mu-Jian
3Xia, Li-Jian
4Yang, Ming-Yu
5Liu, Qing-Gen
6Chen, Jing-Bo
jtitleInternational Journal of Molecular Medicine
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Xi
1Zhuang
2Teng
3Xia
4Yang
5Liu
6Chen
aufirst
0Xiang-Peng
1Jing
2Mu-Jian
3Li-Jian
4Ming-Yu
5Qing-Gen
6Jing-Bo
au
0Xi, Xiang-Peng
1Zhuang, Jing
2Teng, Mu-Jian
3Xia, Li-Jian
4Yang, Ming-Yu
5Liu, Qing-Gen
6Chen, Jing-Bo
atitleMicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer
jtitleInternational Journal of Molecular Medicine
risdate201608
volume38
issue2
spage499
epage506
pages499-506
issn1107-3756
eissn1791-244X
formatjournal
genrearticle
ristypeJOUR
abstractMicroRNA-17 (miRNA-17/miR-17) expression has been confirmed to be significantly higher in colorectal cancer tissues than in normal tissues. However, its exact role in colorectal cancer has not yet been fully elucidated. In this study, we found that miR-17 not only promoted epithelial-mesenchymal transition (EMT), but also promoted the formation of a stem cell-like population in colon cancer DLD1 cells. We also wished to determine the role of cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) in CRC. miR-17 was overexpressed using a recombinant plasmid and CYP7B1 was silenced by transfection with shRNA. Western blot analysis was used to determine protein expression in the DLD1 cells and in tumor tissues obtained from patients with colon cancer. Our results revealed that miR-17 overexpression led to the degradation of CYP7B1 mRNA expression in DLD1 cells. In addition, we found that the silencing of CYB7B1 promoted EMT and the formation of a stem cell-like population in the cells. Thus, our findings demonstrate that miR-17 induces EMT consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer.
pubD.A. Spandidos
doi10.3892/ijmm.2016.2624
date2016-08