schliessen

Filtern

 

Bibliotheken

Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells

Dendritic cell (DC)-based cancer immunotherapy requires an immunogenic tumor-associated antigen and an effective therapeutic strategy. Glypican 3 (GPC3) is a valuable diagnostic marker and a potential therapeutic target in hepatocellular carcinoma (HCC). The present study investigated whether DCs tr... Full description

Journal Title: Molecular Medicine Reports 5/2015, Vol.11(5), pp.3361-3367
Main Author: Wang, Yuliang
Other Authors: Wang, Yinlong , Mu, Hong , Liu, Tao , Chen, Xiaobo , Shen, Zhongyang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1791-2997 ; E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2015.3239
Link: http://www.spandidos-publications.com/molecular medicine reports/11/5/3361
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: spandido10.3892/mmr.2015.3239
title: Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells
format: Article
creator:
  • Wang, Yuliang
  • Wang, Yinlong
  • Mu, Hong
  • Liu, Tao
  • Chen, Xiaobo
  • Shen, Zhongyang
subjects:
  • Dendritic Cells
  • Cytokine-Induced Killer Cells
  • Hepatocellular Carcinoma
  • Glypican 3
  • Cytotoxicity
ispartof: Molecular Medicine Reports, 5/2015, Vol.11(5), pp.3361-3367
description: Dendritic cell (DC)-based cancer immunotherapy requires an immunogenic tumor-associated antigen and an effective therapeutic strategy. Glypican 3 (GPC3) is a valuable diagnostic marker and a potential therapeutic target in hepatocellular carcinoma (HCC). The present study investigated whether DCs transduced with the GPC3 gene (DCs-GPC3) and co-cultured with autologous cytokine-induced killer cells (CIKs) may induce a marked specific immune response against GPC3-expressing HCC cells in vitro and in vivo . Human DCs were transfected with a green fluorescent protein plasmid with GPC3 by nucleofection and then co-cultured with autologous CIKs. Flow cytometry was used to measure the phenotypes of DCs and CIKs. The co-cultured cells were harvested and incubated with HCC cells and the cytotoxicity of the CIKs was assessed by nonradioactive cytotoxicity assay. The anti-tumor activity of these effector cells was further evaluated using a nude mouse tumor model. The results demonstrated that DCs-GPC3 significantly promoted the autologous CIKs differentiation, as well as anti-tumor cytokine interferon-γ secretion. In addition, DCs-GPC3-CIKs significantly enhanced the cytotoxic activity against GPC3-expressing HepG2 cells, indicating a GPC3-specific marked immune response against HCC cells. The in vivo data indicated that DCs-GPC3-CIKs exhibited significant HepG2 cell-induced tumor growth inhibition in nude mice. The results of the present study provided a new insight into the design of personalizing adoptive immunotherapy for GPC3-expressing HCC cells.
language: eng
source:
identifier: ISSN: 1791-2997 ; E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2015.3239
fulltext: fulltext
issn:
  • 1791-2997
  • 17912997
  • 1791-3004
  • 17913004
url: Link


@attributes
ID642590711
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.3892/mmr.2015.3239
sourceidspandido
recordidTN_spandido10.3892/mmr.2015.3239
sourceformatXML
sourcesystemPC
pqid1658416512
galeid420928616
display
typearticle
titleEnhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells
creatorWang, Yuliang ; Wang, Yinlong ; Mu, Hong ; Liu, Tao ; Chen, Xiaobo ; Shen, Zhongyang
ispartofMolecular Medicine Reports, 5/2015, Vol.11(5), pp.3361-3367
identifier
subjectDendritic Cells ; Cytokine-Induced Killer Cells ; Hepatocellular Carcinoma ; Glypican 3 ; Cytotoxicity
descriptionDendritic cell (DC)-based cancer immunotherapy requires an immunogenic tumor-associated antigen and an effective therapeutic strategy. Glypican 3 (GPC3) is a valuable diagnostic marker and a potential therapeutic target in hepatocellular carcinoma (HCC). The present study investigated whether DCs transduced with the GPC3 gene (DCs-GPC3) and co-cultured with autologous cytokine-induced killer cells (CIKs) may induce a marked specific immune response against GPC3-expressing HCC cells in vitro and in vivo . Human DCs were transfected with a green fluorescent protein plasmid with GPC3 by nucleofection and then co-cultured with autologous CIKs. Flow cytometry was used to measure the phenotypes of DCs and CIKs. The co-cultured cells were harvested and incubated with HCC cells and the cytotoxicity of the CIKs was assessed by nonradioactive cytotoxicity assay. The anti-tumor activity of these effector cells was further evaluated using a nude mouse tumor model. The results demonstrated that DCs-GPC3 significantly promoted the autologous CIKs differentiation, as well as anti-tumor cytokine interferon-γ secretion. In addition, DCs-GPC3-CIKs significantly enhanced the cytotoxic activity against GPC3-expressing HepG2 cells, indicating a GPC3-specific marked immune response against HCC cells. The in vivo data indicated that DCs-GPC3-CIKs exhibited significant HepG2 cell-induced tumor growth inhibition in nude mice. The results of the present study provided a new insight into the design of personalizing adoptive immunotherapy for GPC3-expressing HCC cells.
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://www.spandidos-publications.com/molecular medicine reports/11/5/3361$$EView_this_record_in_Spandidos_Publications
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0WANG, YULIANG
1WANG, YINLONG
2MU, HONG
3LIU, TAO
4CHEN, XIAOBO
5SHEN, ZHONGYANG
titleEnhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells
descriptionDendritic cell (DC)-based cancer immunotherapy requires an immunogenic tumor-associated antigen and an effective therapeutic strategy. Glypican 3 (GPC3) is a valuable diagnostic marker and a potential therapeutic target in hepatocellular carcinoma (HCC). The present study investigated whether DCs transduced with the GPC3 gene (DCs-GPC3) and co-cultured with autologous cytokine-induced killer cells (CIKs) may induce a marked specific immune response against GPC3-expressing HCC cells in vitro and in vivo . Human DCs were transfected with a green fluorescent protein plasmid with GPC3 by nucleofection and then co-cultured with autologous CIKs. Flow cytometry was used to measure the phenotypes of DCs and CIKs. The co-cultured cells were harvested and incubated with HCC cells and the cytotoxicity of the CIKs was assessed by nonradioactive cytotoxicity assay. The anti-tumor activity of these effector cells was further evaluated using a nude mouse tumor model. The results demonstrated that DCs-GPC3 significantly promoted the autologous CIKs differentiation, as well as anti-tumor cytokine interferon-γ secretion. In addition, DCs-GPC3-CIKs significantly enhanced the cytotoxic activity against GPC3-expressing HepG2 cells, indicating a GPC3-specific marked immune response against HCC cells. The in vivo data indicated that DCs-GPC3-CIKs exhibited significant HepG2 cell-induced tumor growth inhibition in nude mice. The results of the present study provided a new insight into the design of personalizing adoptive immunotherapy for GPC3-expressing HCC cells.
subject
0dendritic cells
1cytokine-induced killer cells
2hepatocellular carcinoma
3glypican 3
4cytotoxicity
general
010.3892/mmr.2015.3239
1D.A. Spandidos
2English
3Spandidios Publications (Spandidos Publications Ltd.)
sourceidspandido
recordidspandido10.3892/mmr.2015.3239
issn
01791-2997
117912997
21791-3004
317913004
rsrctypearticle
creationdate2015
addtitleMolecular Medicine Reports
searchscopespandidos
scopespandidos
lsr30VSR-Enriched:[pqid, galeid]
sort
titleEnhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells
authorWang, Yuliang ; Wang, Yinlong ; Mu, Hong ; Liu, Tao ; Chen, Xiaobo ; Shen, Zhongyang
facets
frbrgroupid8125014217838382633
frbrtype5
languageeng
creationdate2015
topic
0dendritic cells
1cytokine-induced killer cells
2hepatocellular carcinoma
3glypican 3
4cytotoxicity
collectionSpandidios Publications (Spandidos Publications Ltd.)
prefilterarticles
rsrctypearticles
creatorcontrib
0Wang, Yuliang
1Wang, Yinlong
2Mu, Hong
3Liu, Tao
4Chen, Xiaobo
5Shen, Zhongyang
jtitleMolecular Medicine Reports
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0WANG
1MU
2LIU
3CHEN
4SHEN
aufirst
0YULIANG
1YINLONG
2HONG
3TAO
4XIAOBO
5ZHONGYANG
au
0Wang, Yuliang
1Wang, Yinlong
2Mu, Hong
3Liu, Tao
4Chen, Xiaobo
5Shen, Zhongyang
atitleEnhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells
jtitleMolecular Medicine Reports
date20155
risdate20155
volume11
issue5
spage3361
epage3367
pages3361-3367
issn1791-2997
eissn1791-3004
formatjournal
genrearticle
ristypeJOUR
abstractDendritic cell (DC)-based cancer immunotherapy requires an immunogenic tumor-associated antigen and an effective therapeutic strategy. Glypican 3 (GPC3) is a valuable diagnostic marker and a potential therapeutic target in hepatocellular carcinoma (HCC). The present study investigated whether DCs transduced with the GPC3 gene (DCs-GPC3) and co-cultured with autologous cytokine-induced killer cells (CIKs) may induce a marked specific immune response against GPC3-expressing HCC cells in vitro and in vivo . Human DCs were transfected with a green fluorescent protein plasmid with GPC3 by nucleofection and then co-cultured with autologous CIKs. Flow cytometry was used to measure the phenotypes of DCs and CIKs. The co-cultured cells were harvested and incubated with HCC cells and the cytotoxicity of the CIKs was assessed by nonradioactive cytotoxicity assay. The anti-tumor activity of these effector cells was further evaluated using a nude mouse tumor model. The results demonstrated that DCs-GPC3 significantly promoted the autologous CIKs differentiation, as well as anti-tumor cytokine interferon-γ secretion. In addition, DCs-GPC3-CIKs significantly enhanced the cytotoxic activity against GPC3-expressing HepG2 cells, indicating a GPC3-specific marked immune response against HCC cells. The in vivo data indicated that DCs-GPC3-CIKs exhibited significant HepG2 cell-induced tumor growth inhibition in nude mice. The results of the present study provided a new insight into the design of personalizing adoptive immunotherapy for GPC3-expressing HCC cells.
pubD.A. Spandidos
doi10.3892/mmr.2015.3239
Cannot write session to /tmp/vufind_sessions/sess_glv6dok3k8i5bf200a0op22c06