schliessen

Filtern

 

Bibliotheken

Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro

The widely-used Chinese medicinal herb Hypericum japonicum , also known as Hypericum japonicum Thunb or Tianjihuang, displays potent anti-carcinogenic effects against liver cancer. However, the molecular mechanism underlying the therapeutic effects of Hypericum japonicum remains to be elucidated. Th... Full description

Journal Title: Molecular Medicine Reports 10/2015, Vol.12(4), pp.4851-4858
Main Author: Zhuang, Qunchuan
Other Authors: Li, Jing , Chen, Youqin , Lin, Jiumao , Lai, Faze , Chen, Xuzheng , Lin, Xindeng , Peng, Jun
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1791-2997 ; E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2015.4086
Link: http://www.spandidos-publications.com/molecular medicine reports/12/4/4851
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: spandido10.3892/mmr.2015.4086
title: Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro
format: Article
creator:
  • Zhuang, Qunchuan
  • Li, Jing
  • Chen, Youqin
  • Lin, Jiumao
  • Lai, Faze
  • Chen, Xuzheng
  • Lin, Xindeng
  • Peng, Jun
subjects:
  • Apoptosis
  • Liver Cancer
  • Hepg2 Cells
  • Herbal Medicine
  • Mitochondria
  • Hypericum Japonicum
ispartof: Molecular Medicine Reports, 10/2015, Vol.12(4), pp.4851-4858
description: The widely-used Chinese medicinal herb Hypericum japonicum , also known as Hypericum japonicum Thunb or Tianjihuang, displays potent anti-carcinogenic effects against liver cancer. However, the molecular mechanism underlying the therapeutic effects of Hypericum japonicum remains to be elucidated. The present study investigated the in vivo efficacy of ethyl acetate extract of Hypericum japonicum (EAEHJ) against tumor growth in an H22 cell-bearing liver cancer mouse model. Treatment with EAEHJ significantly reduced tumor weight, but had no effect on murine body weight. The results of the present study also showed that EAEHJ induced H22 cell apoptosis in vivo . In addition, the anti-carcinogenic effects of EAEHJ were investigated in vitro . The results of the present study demonstrate that both phospholipid asymmetry in the plasma membrane and mitochondrial membrane potential were deregulated in HepG2 human hepatoma cells, following treatment with EAEHJ. Treatment with EAEHJ also increased the ratio of pro-apoptotic B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) to anti-apoptotic Bcl-2, and activated the caspase-9 signaling pathway. These results suggest that EAEHJ is able to trigger the apoptosis of liver cancer cells via the mitochondria-dependent pathway.
language: eng
source:
identifier: ISSN: 1791-2997 ; E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2015.4086
fulltext: fulltext
issn:
  • 1791-2997
  • 17912997
  • 1791-3004
  • 17913004
url: Link


@attributes
ID85658650
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.3892/mmr.2015.4086
sourceidspandido
recordidTN_spandido10.3892/mmr.2015.4086
sourceformatXML
sourcesystemPC
pqid1716933716
galeid432495567
display
typearticle
titleEthyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro
creatorZhuang, Qunchuan ; Li, Jing ; Chen, Youqin ; Lin, Jiumao ; Lai, Faze ; Chen, Xuzheng ; Lin, Xindeng ; Peng, Jun
ispartofMolecular Medicine Reports, 10/2015, Vol.12(4), pp.4851-4858
identifier
subjectApoptosis ; Liver Cancer ; Hepg2 Cells ; Herbal Medicine ; Mitochondria ; Hypericum Japonicum
descriptionThe widely-used Chinese medicinal herb Hypericum japonicum , also known as Hypericum japonicum Thunb or Tianjihuang, displays potent anti-carcinogenic effects against liver cancer. However, the molecular mechanism underlying the therapeutic effects of Hypericum japonicum remains to be elucidated. The present study investigated the in vivo efficacy of ethyl acetate extract of Hypericum japonicum (EAEHJ) against tumor growth in an H22 cell-bearing liver cancer mouse model. Treatment with EAEHJ significantly reduced tumor weight, but had no effect on murine body weight. The results of the present study also showed that EAEHJ induced H22 cell apoptosis in vivo . In addition, the anti-carcinogenic effects of EAEHJ were investigated in vitro . The results of the present study demonstrate that both phospholipid asymmetry in the plasma membrane and mitochondrial membrane potential were deregulated in HepG2 human hepatoma cells, following treatment with EAEHJ. Treatment with EAEHJ also increased the ratio of pro-apoptotic B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) to anti-apoptotic Bcl-2, and activated the caspase-9 signaling pathway. These results suggest that EAEHJ is able to trigger the apoptosis of liver cancer cells via the mitochondria-dependent pathway.
languageeng
source
version8
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://www.spandidos-publications.com/molecular medicine reports/12/4/4851$$EView_this_record_in_Spandidos_Publications
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0ZHUANG, QUNCHUAN
1LI, JING
2CHEN, YOUQIN
3LIN, JIUMAO
4LAI, FAZE
5CHEN, XUZHENG
6LIN, XINDENG
7PENG, JUN
titleEthyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro
descriptionThe widely-used Chinese medicinal herb Hypericum japonicum , also known as Hypericum japonicum Thunb or Tianjihuang, displays potent anti-carcinogenic effects against liver cancer. However, the molecular mechanism underlying the therapeutic effects of Hypericum japonicum remains to be elucidated. The present study investigated the in vivo efficacy of ethyl acetate extract of Hypericum japonicum (EAEHJ) against tumor growth in an H22 cell-bearing liver cancer mouse model. Treatment with EAEHJ significantly reduced tumor weight, but had no effect on murine body weight. The results of the present study also showed that EAEHJ induced H22 cell apoptosis in vivo . In addition, the anti-carcinogenic effects of EAEHJ were investigated in vitro . The results of the present study demonstrate that both phospholipid asymmetry in the plasma membrane and mitochondrial membrane potential were deregulated in HepG2 human hepatoma cells, following treatment with EAEHJ. Treatment with EAEHJ also increased the ratio of pro-apoptotic B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) to anti-apoptotic Bcl-2, and activated the caspase-9 signaling pathway. These results suggest that EAEHJ is able to trigger the apoptosis of liver cancer cells via the mitochondria-dependent pathway.
subject
0apoptosis
1liver cancer
2HepG2 cells
3herbal medicine
4mitochondria
5Hypericum japonicum
general
010.3892/mmr.2015.4086
1D.A. Spandidos
2English
3Spandidios Publications (Spandidos Publications Ltd.)
sourceidspandido
recordidspandido10.3892/mmr.2015.4086
issn
01791-2997
117912997
21791-3004
317913004
rsrctypearticle
creationdate2015
addtitleMolecular Medicine Reports
searchscopespandidos
scopespandidos
lsr30VSR-Enriched:[galeid, pqid]
sort
titleEthyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro
authorZhuang, Qunchuan ; Li, Jing ; Chen, Youqin ; Lin, Jiumao ; Lai, Faze ; Chen, Xuzheng ; Lin, Xindeng ; Peng, Jun
creationdate20151000
lso0120151000
facets
frbrgroupid5158207064289253549
frbrtype5
languageeng
creationdate2015
topic
0apoptosis
1liver cancer
2HepG2 cells
3herbal medicine
4mitochondria
5Hypericum japonicum
collectionSpandidios Publications (Spandidos Publications Ltd.)
prefilterarticles
rsrctypearticles
creatorcontrib
0Zhuang, Qunchuan
1Li, Jing
2Chen, Youqin
3Lin, Jiumao
4Lai, Faze
5Chen, Xuzheng
6Lin, Xindeng
7Peng, Jun
jtitleMolecular Medicine Reports
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0ZHUANG
1LI
2CHEN
3LIN
4LAI
5PENG
aufirst
0QUNCHUAN
1JING
2YOUQIN
3JIUMAO
4FAZE
5XUZHENG
6XINDENG
7JUN
au
0Zhuang, Qunchuan
1Li, Jing
2Chen, Youqin
3Lin, Jiumao
4Lai, Faze
5Chen, Xuzheng
6Lin, Xindeng
7Peng, Jun
atitleEthyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro
jtitleMolecular Medicine Reports
risdate201510
volume12
issue4
spage4851
epage4858
pages4851-4858
issn1791-2997
eissn1791-3004
formatjournal
genrearticle
ristypeJOUR
abstractThe widely-used Chinese medicinal herb Hypericum japonicum , also known as Hypericum japonicum Thunb or Tianjihuang, displays potent anti-carcinogenic effects against liver cancer. However, the molecular mechanism underlying the therapeutic effects of Hypericum japonicum remains to be elucidated. The present study investigated the in vivo efficacy of ethyl acetate extract of Hypericum japonicum (EAEHJ) against tumor growth in an H22 cell-bearing liver cancer mouse model. Treatment with EAEHJ significantly reduced tumor weight, but had no effect on murine body weight. The results of the present study also showed that EAEHJ induced H22 cell apoptosis in vivo . In addition, the anti-carcinogenic effects of EAEHJ were investigated in vitro . The results of the present study demonstrate that both phospholipid asymmetry in the plasma membrane and mitochondrial membrane potential were deregulated in HepG2 human hepatoma cells, following treatment with EAEHJ. Treatment with EAEHJ also increased the ratio of pro-apoptotic B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) to anti-apoptotic Bcl-2, and activated the caspase-9 signaling pathway. These results suggest that EAEHJ is able to trigger the apoptosis of liver cancer cells via the mitochondria-dependent pathway.
pubD.A. Spandidos
doi10.3892/mmr.2015.4086
date2015-10