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Autologous adipose tissue-derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy

Adipose tissue-derived mesenchymal stem cells (ADSCs) have been considered to be attractive and readily available adult mesenchymal stem cells, and they are becoming increasingly popular for use in regenerative cell therapy, as they are readily accessible through minimally invasive techniques. The p... Full description

Journal Title: Molecular medicine reports 2016-03, Vol.13 (3), p.2053-2059
Main Author: LIU, TAO
Other Authors: MU, HONG , SHEN, ZHONGYANG , SONG, ZHUOLUN , CHEN, XIAOBO , WANG, YULIANG
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Greece: D.A. Spandidos
ID: ISSN: 1791-2997
Link: https://www.ncbi.nlm.nih.gov/pubmed/26783183
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recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4769001
title: Autologous adipose tissue-derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy
format: Article
creator:
  • LIU, TAO
  • MU, HONG
  • SHEN, ZHONGYANG
  • SONG, ZHUOLUN
  • CHEN, XIAOBO
  • WANG, YULIANG
subjects:
  • Adipose tissue
  • Adipose Tissue - cytology
  • adipose tissue-derived mesenchymal stem cells
  • Alanine
  • Alanine transaminase
  • Allografts
  • Animals
  • Antigens
  • Articles
  • Aspartate aminotransferase
  • Autografts
  • Bilirubin
  • Body weight
  • Cell Shape
  • Degeneration
  • derived mesenchymal stem cells
  • Gene Expression Regulation
  • Hepatectomy
  • Hepatocyte growth factor
  • Hepatocyte Growth Factor - genetics
  • Hepatocyte Growth Factor - metabolism
  • Humidity
  • Immunohistochemistry
  • Laboratory animals
  • Liver
  • Liver - pathology
  • Liver - physiopathology
  • Liver - surgery
  • Liver Function Tests
  • Liver Regeneration
  • Liver transplantation
  • Male
  • Mesenchymal Stromal Cells - cytology
  • Mesenchyme
  • Models
  • Observations
  • Patient outcomes
  • Phenotype
  • Polymerase chain reaction
  • Portal vein
  • Proliferating cell nuclear antigen
  • Proliferating Cell Nuclear Antigen - metabolism
  • Rats, Wistar
  • Regeneration
  • repeat partial hepatectomy
  • Reverse transcription
  • RNA, Messenger - genetics
  • RNA, Messenger - metabolism
  • Rodents
  • Serum levels
  • Stem cell transplantation
  • Stem cells
  • Studies
  • Surgery
  • Syngeneic grafts
  • Transplantation
  • Transplantation, Autologous
ispartof: Molecular medicine reports, 2016-03, Vol.13 (3), p.2053-2059
description: Adipose tissue-derived mesenchymal stem cells (ADSCs) have been considered to be attractive and readily available adult mesenchymal stem cells, and they are becoming increasingly popular for use in regenerative cell therapy, as they are readily accessible through minimally invasive techniques. The present study investigated whether autologous ADSC transplantation promoted liver regeneration following a repeat partial hepatectomy in rats. The rats were divided into three groups as follows: 70% partial hepatectomy (PH) group; repeat PH (R-PH) group and R-PH/ADSC group, subjected to R-PH and treated with autologous ADSCs via portal vein injection. In each group, the rats were sacrificed at different time points postoperatively in order to evaluate the changes in liver function and to estimate the liver regenerative response. The expression of proliferating cell nuclear antigen (PCNA) labeling index in the liver was measured using immunohistochemistry. The expression levels of hepatocyte growth factor (HGF) mRNA were measured using reverse transcription polymerase chain reaction. The results showed that regeneration of the remaining liver following R-PH was significantly promoted by ADSC transplantation, as shown by a significant increase in liver to body weight ratio and the PCNA labeling index at 24 h post-hepatectomy. Additionally, ADSC transplantation markedly inhibited the elevation of serum levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin, increased HGF content and also attenuated hepatic vacuolar degeneration 24 h postoperatively. Furthermore, the liver was found to almost fully recover from hepatocellular damage due to hepatectomy among the three groups at 168 h postoperatively. These results indicated that autologous ADSC transplantation enhanced the regenerative capacity of the remnant liver tissues in the early phase following R-PH.
language: eng
source:
identifier: ISSN: 1791-2997
fulltext: no_fulltext
issn:
  • 1791-2997
  • 1791-3004
url: Link


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titleAutologous adipose tissue-derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy
creatorLIU, TAO ; MU, HONG ; SHEN, ZHONGYANG ; SONG, ZHUOLUN ; CHEN, XIAOBO ; WANG, YULIANG
creatorcontribLIU, TAO ; MU, HONG ; SHEN, ZHONGYANG ; SONG, ZHUOLUN ; CHEN, XIAOBO ; WANG, YULIANG
descriptionAdipose tissue-derived mesenchymal stem cells (ADSCs) have been considered to be attractive and readily available adult mesenchymal stem cells, and they are becoming increasingly popular for use in regenerative cell therapy, as they are readily accessible through minimally invasive techniques. The present study investigated whether autologous ADSC transplantation promoted liver regeneration following a repeat partial hepatectomy in rats. The rats were divided into three groups as follows: 70% partial hepatectomy (PH) group; repeat PH (R-PH) group and R-PH/ADSC group, subjected to R-PH and treated with autologous ADSCs via portal vein injection. In each group, the rats were sacrificed at different time points postoperatively in order to evaluate the changes in liver function and to estimate the liver regenerative response. The expression of proliferating cell nuclear antigen (PCNA) labeling index in the liver was measured using immunohistochemistry. The expression levels of hepatocyte growth factor (HGF) mRNA were measured using reverse transcription polymerase chain reaction. The results showed that regeneration of the remaining liver following R-PH was significantly promoted by ADSC transplantation, as shown by a significant increase in liver to body weight ratio and the PCNA labeling index at 24 h post-hepatectomy. Additionally, ADSC transplantation markedly inhibited the elevation of serum levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin, increased HGF content and also attenuated hepatic vacuolar degeneration 24 h postoperatively. Furthermore, the liver was found to almost fully recover from hepatocellular damage due to hepatectomy among the three groups at 168 h postoperatively. These results indicated that autologous ADSC transplantation enhanced the regenerative capacity of the remnant liver tissues in the early phase following R-PH.
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languageeng
publisherGreece: D.A. Spandidos
subjectAdipose tissue ; Adipose Tissue - cytology ; adipose tissue-derived mesenchymal stem cells ; Alanine ; Alanine transaminase ; Allografts ; Animals ; Antigens ; Articles ; Aspartate aminotransferase ; Autografts ; Bilirubin ; Body weight ; Cell Shape ; Degeneration ; derived mesenchymal stem cells ; Gene Expression Regulation ; Hepatectomy ; Hepatocyte growth factor ; Hepatocyte Growth Factor - genetics ; Hepatocyte Growth Factor - metabolism ; Humidity ; Immunohistochemistry ; Laboratory animals ; Liver ; Liver - pathology ; Liver - physiopathology ; Liver - surgery ; Liver Function Tests ; Liver Regeneration ; Liver transplantation ; Male ; Mesenchymal Stromal Cells - cytology ; Mesenchyme ; Models ; Observations ; Patient outcomes ; Phenotype ; Polymerase chain reaction ; Portal vein ; Proliferating cell nuclear antigen ; Proliferating Cell Nuclear Antigen - metabolism ; Rats, Wistar ; Regeneration ; repeat partial hepatectomy ; Reverse transcription ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Rodents ; Serum levels ; Stem cell transplantation ; Stem cells ; Studies ; Surgery ; Syngeneic grafts ; Transplantation ; Transplantation, Autologous
ispartofMolecular medicine reports, 2016-03, Vol.13 (3), p.2053-2059
rights
0Copyright: © Liu et al.
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1MU, HONG
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0Autologous adipose tissue-derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy
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descriptionAdipose tissue-derived mesenchymal stem cells (ADSCs) have been considered to be attractive and readily available adult mesenchymal stem cells, and they are becoming increasingly popular for use in regenerative cell therapy, as they are readily accessible through minimally invasive techniques. The present study investigated whether autologous ADSC transplantation promoted liver regeneration following a repeat partial hepatectomy in rats. The rats were divided into three groups as follows: 70% partial hepatectomy (PH) group; repeat PH (R-PH) group and R-PH/ADSC group, subjected to R-PH and treated with autologous ADSCs via portal vein injection. In each group, the rats were sacrificed at different time points postoperatively in order to evaluate the changes in liver function and to estimate the liver regenerative response. The expression of proliferating cell nuclear antigen (PCNA) labeling index in the liver was measured using immunohistochemistry. The expression levels of hepatocyte growth factor (HGF) mRNA were measured using reverse transcription polymerase chain reaction. The results showed that regeneration of the remaining liver following R-PH was significantly promoted by ADSC transplantation, as shown by a significant increase in liver to body weight ratio and the PCNA labeling index at 24 h post-hepatectomy. Additionally, ADSC transplantation markedly inhibited the elevation of serum levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin, increased HGF content and also attenuated hepatic vacuolar degeneration 24 h postoperatively. Furthermore, the liver was found to almost fully recover from hepatocellular damage due to hepatectomy among the three groups at 168 h postoperatively. These results indicated that autologous ADSC transplantation enhanced the regenerative capacity of the remnant liver tissues in the early phase following R-PH.
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3Alanine
4Alanine transaminase
5Allografts
6Animals
7Antigens
8Articles
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11Bilirubin
12Body weight
13Cell Shape
14Degeneration
15derived mesenchymal stem cells
16Gene Expression Regulation
17Hepatectomy
18Hepatocyte growth factor
19Hepatocyte Growth Factor - genetics
20Hepatocyte Growth Factor - metabolism
21Humidity
22Immunohistochemistry
23Laboratory animals
24Liver
25Liver - pathology
26Liver - physiopathology
27Liver - surgery
28Liver Function Tests
29Liver Regeneration
30Liver transplantation
31Male
32Mesenchymal Stromal Cells - cytology
33Mesenchyme
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35Observations
36Patient outcomes
37Phenotype
38Polymerase chain reaction
39Portal vein
40Proliferating cell nuclear antigen
41Proliferating Cell Nuclear Antigen - metabolism
42Rats, Wistar
43Regeneration
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45Reverse transcription
46RNA, Messenger - genetics
47RNA, Messenger - metabolism
48Rodents
49Serum levels
50Stem cell transplantation
51Stem cells
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titleAutologous adipose tissue-derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy
authorLIU, TAO ; MU, HONG ; SHEN, ZHONGYANG ; SONG, ZHUOLUN ; CHEN, XIAOBO ; WANG, YULIANG
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1Adipose Tissue - cytology
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3Alanine
4Alanine transaminase
5Allografts
6Animals
7Antigens
8Articles
9Aspartate aminotransferase
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11Bilirubin
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16Gene Expression Regulation
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18Hepatocyte growth factor
19Hepatocyte Growth Factor - genetics
20Hepatocyte Growth Factor - metabolism
21Humidity
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29Liver Regeneration
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33Mesenchyme
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35Observations
36Patient outcomes
37Phenotype
38Polymerase chain reaction
39Portal vein
40Proliferating cell nuclear antigen
41Proliferating Cell Nuclear Antigen - metabolism
42Rats, Wistar
43Regeneration
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45Reverse transcription
46RNA, Messenger - genetics
47RNA, Messenger - metabolism
48Rodents
49Serum levels
50Stem cell transplantation
51Stem cells
52Studies
53Surgery
54Syngeneic grafts
55Transplantation
56Transplantation, Autologous
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abstractAdipose tissue-derived mesenchymal stem cells (ADSCs) have been considered to be attractive and readily available adult mesenchymal stem cells, and they are becoming increasingly popular for use in regenerative cell therapy, as they are readily accessible through minimally invasive techniques. The present study investigated whether autologous ADSC transplantation promoted liver regeneration following a repeat partial hepatectomy in rats. The rats were divided into three groups as follows: 70% partial hepatectomy (PH) group; repeat PH (R-PH) group and R-PH/ADSC group, subjected to R-PH and treated with autologous ADSCs via portal vein injection. In each group, the rats were sacrificed at different time points postoperatively in order to evaluate the changes in liver function and to estimate the liver regenerative response. The expression of proliferating cell nuclear antigen (PCNA) labeling index in the liver was measured using immunohistochemistry. The expression levels of hepatocyte growth factor (HGF) mRNA were measured using reverse transcription polymerase chain reaction. The results showed that regeneration of the remaining liver following R-PH was significantly promoted by ADSC transplantation, as shown by a significant increase in liver to body weight ratio and the PCNA labeling index at 24 h post-hepatectomy. Additionally, ADSC transplantation markedly inhibited the elevation of serum levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin, increased HGF content and also attenuated hepatic vacuolar degeneration 24 h postoperatively. Furthermore, the liver was found to almost fully recover from hepatocellular damage due to hepatectomy among the three groups at 168 h postoperatively. These results indicated that autologous ADSC transplantation enhanced the regenerative capacity of the remnant liver tissues in the early phase following R-PH.
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