schliessen

Filtern

 

Bibliotheken

Identification of therapeutic targets for Alzheimer's disease via differentially expressed gene and weighted gene co-expression network analyses

In order to investigate the pathogenic targets and associated biological process of Alzheimer's disease in the present study, mRNA expression profiles (GSE28146) and microRNA (miRNA) expression profiles (GSE16759) were downloaded from the Gene Expression Omnibus database. In GSE28146, eight control... Full description

Journal Title: Molecular Medicine Reports 11/2016, Vol.14(5), pp.4844-4848
Main Author: Jia, Yujie
Other Authors: Nie, Kun , Li, Jing , Liang, Xinyue , Zhang, Xuezhu
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1791-2997 ; E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2016.5828
Link: http://www.spandidos-publications.com/mmr/14/5/4844
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: spandido10.3892/mmr.2016.5828
title: Identification of therapeutic targets for Alzheimer's disease via differentially expressed gene and weighted gene co-expression network analyses
format: Article
creator:
  • Jia, Yujie
  • Nie, Kun
  • Li, Jing
  • Liang, Xinyue
  • Zhang, Xuezhu
subjects:
  • Alzheimer'S Disease
  • Gene Expression Omnibus
  • Pathogenic Targets
  • Neurological Diseases
ispartof: Molecular Medicine Reports, 11/2016, Vol.14(5), pp.4844-4848
description: In order to investigate the pathogenic targets and associated biological process of Alzheimer's disease in the present study, mRNA expression profiles (GSE28146) and microRNA (miRNA) expression profiles (GSE16759) were downloaded from the Gene Expression Omnibus database. In GSE28146, eight control samples, and Alzheimer's disease samples comprising seven incipient, eight moderate, seven severe Alzheimer's disease samples, were included. The Affy package in R was used for background correction and normalization of the raw microarray data. The differentially expressed genes (DEGs) and differentially expressed miRNAs were identified using the Limma package. In addition, mRNAs were clustered using weighted gene correlation network analysis, and modules found to be significantly associated with the stages of Alzheimer's disease were screened out. The Database for Annotation, Visualization, and Integrated Discovery was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. The target genes of the differentially expressed miRNAs were identified using the miRWalk database. Compared with the control samples, 175,59 genes and 90 DEGs were identified in the incipient, moderate and severe Alzheimer's disease samples, respectively. A module, which contained 1,592 genes was found to be closely associated with the stage of Alzheimer's disease and biological processes. In addition, pathways associated with Alzheimer's disease and other neurological diseases were found to be enriched in those genes. A total of 139 overlapped genes were identified between those genes and the DEGs in the three groups. From the miRNA expression profiles, 189 miRNAs were found differentially expressed in the samples from patients with Alzheimer's disease and 1,647 target genes were obtained. In addition, five overlapped genes were identified between those 1,647 target genes and the 139 genes, and these genes may be important pathogenic targets for Alzheimer's disease. Through bioinformatics analysis, potential therapeutic targets and associated biological processes were identified, which may assist in understanding and treatment of Alzheimer's disease.
language: eng
source:
identifier: ISSN: 1791-2997 ; E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2016.5828
fulltext: fulltext
issn:
  • 1791-2997
  • 17912997
  • 1791-3004
  • 17913004
url: Link


@attributes
ID524756863
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.3892/mmr.2016.5828
sourceidspandido
recordidTN_spandido10.3892/mmr.2016.5828
sourceformatXML
sourcesystemPC
pqid1835443973
galeid472989138
display
typearticle
titleIdentification of therapeutic targets for Alzheimer's disease via differentially expressed gene and weighted gene co-expression network analyses
creatorJia, Yujie ; Nie, Kun ; Li, Jing ; Liang, Xinyue ; Zhang, Xuezhu
ispartofMolecular Medicine Reports, 11/2016, Vol.14(5), pp.4844-4848
identifier
subjectAlzheimer'S Disease ; Gene Expression Omnibus ; Pathogenic Targets ; Neurological Diseases
descriptionIn order to investigate the pathogenic targets and associated biological process of Alzheimer's disease in the present study, mRNA expression profiles (GSE28146) and microRNA (miRNA) expression profiles (GSE16759) were downloaded from the Gene Expression Omnibus database. In GSE28146, eight control samples, and Alzheimer's disease samples comprising seven incipient, eight moderate, seven severe Alzheimer's disease samples, were included. The Affy package in R was used for background correction and normalization of the raw microarray data. The differentially expressed genes (DEGs) and differentially expressed miRNAs were identified using the Limma package. In addition, mRNAs were clustered using weighted gene correlation network analysis, and modules found to be significantly associated with the stages of Alzheimer's disease were screened out. The Database for Annotation, Visualization, and Integrated Discovery was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. The target genes of the differentially expressed miRNAs were identified using the miRWalk database. Compared with the control samples, 175,59 genes and 90 DEGs were identified in the incipient, moderate and severe Alzheimer's disease samples, respectively. A module, which contained 1,592 genes was found to be closely associated with the stage of Alzheimer's disease and biological processes. In addition, pathways associated with Alzheimer's disease and other neurological diseases were found to be enriched in those genes. A total of 139 overlapped genes were identified between those genes and the DEGs in the three groups. From the miRNA expression profiles, 189 miRNAs were found differentially expressed in the samples from patients with Alzheimer's disease and 1,647 target genes were obtained. In addition, five overlapped genes were identified between those 1,647 target genes and the 139 genes, and these genes may be important pathogenic targets for Alzheimer's disease. Through bioinformatics analysis, potential therapeutic targets and associated biological processes were identified, which may assist in understanding and treatment of Alzheimer's disease.
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://www.spandidos-publications.com/mmr/14/5/4844$$EView_this_record_in_Spandidos_Publications
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Jia, Yujie
1Nie, Kun
2Li, Jing
3Liang, Xinyue
4Zhang, Xuezhu
titleIdentification of therapeutic targets for Alzheimer's disease via differentially expressed gene and weighted gene co-expression network analyses
descriptionIn order to investigate the pathogenic targets and associated biological process of Alzheimer's disease in the present study, mRNA expression profiles (GSE28146) and microRNA (miRNA) expression profiles (GSE16759) were downloaded from the Gene Expression Omnibus database. In GSE28146, eight control samples, and Alzheimer's disease samples comprising seven incipient, eight moderate, seven severe Alzheimer's disease samples, were included. The Affy package in R was used for background correction and normalization of the raw microarray data. The differentially expressed genes (DEGs) and differentially expressed miRNAs were identified using the Limma package. In addition, mRNAs were clustered using weighted gene correlation network analysis, and modules found to be significantly associated with the stages of Alzheimer's disease were screened out. The Database for Annotation, Visualization, and Integrated Discovery was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. The target genes of the differentially expressed miRNAs were identified using the miRWalk database. Compared with the control samples, 175,59 genes and 90 DEGs were identified in the incipient, moderate and severe Alzheimer's disease samples, respectively. A module, which contained 1,592 genes was found to be closely associated with the stage of Alzheimer's disease and biological processes. In addition, pathways associated with Alzheimer's disease and other neurological diseases were found to be enriched in those genes. A total of 139 overlapped genes were identified between those genes and the DEGs in the three groups. From the miRNA expression profiles, 189 miRNAs were found differentially expressed in the samples from patients with Alzheimer's disease and 1,647 target genes were obtained. In addition, five overlapped genes were identified between those 1,647 target genes and the 139 genes, and these genes may be important pathogenic targets for Alzheimer's disease. Through bioinformatics analysis, potential therapeutic targets and associated biological processes were identified, which may assist in understanding and treatment of Alzheimer's disease.
subject
0Alzheimer's disease
1Gene Expression Omnibus
2pathogenic targets
3neurological diseases
general
010.3892/mmr.2016.5828
1D.A. Spandidos
2English
3Spandidios Publications (Spandidos Publications Ltd.)
sourceidspandido
recordidspandido10.3892/mmr.2016.5828
issn
01791-2997
117912997
21791-3004
317913004
rsrctypearticle
creationdate2016
addtitleMolecular Medicine Reports
searchscopespandidos
scopespandidos
lsr30VSR-Enriched:[galeid, pqid]
sort
titleIdentification of therapeutic targets for Alzheimer's disease via differentially expressed gene and weighted gene co-expression network analyses
authorJia, Yujie ; Nie, Kun ; Li, Jing ; Liang, Xinyue ; Zhang, Xuezhu
creationdate20161100
lso0120161100
facets
frbrgroupid7743931735808842156
frbrtype5
languageeng
creationdate2016
topic
0Alzheimer's disease
1Gene Expression Omnibus
2pathogenic targets
3neurological diseases
collectionSpandidios Publications (Spandidos Publications Ltd.)
prefilterarticles
rsrctypearticles
creatorcontrib
0Jia, Yujie
1Nie, Kun
2Li, Jing
3Liang, Xinyue
4Zhang, Xuezhu
jtitleMolecular Medicine Reports
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Jia
1Nie
2Li
3Liang
4Zhang
aufirst
0Yujie
1Kun
2Jing
3Xinyue
4Xuezhu
au
0Jia, Yujie
1Nie, Kun
2Li, Jing
3Liang, Xinyue
4Zhang, Xuezhu
atitleIdentification of therapeutic targets for Alzheimer's disease via differentially expressed gene and weighted gene co-expression network analyses
jtitleMolecular Medicine Reports
risdate201611
volume14
issue5
spage4844
epage4848
pages4844-4848
issn1791-2997
eissn1791-3004
formatjournal
genrearticle
ristypeJOUR
abstractIn order to investigate the pathogenic targets and associated biological process of Alzheimer's disease in the present study, mRNA expression profiles (GSE28146) and microRNA (miRNA) expression profiles (GSE16759) were downloaded from the Gene Expression Omnibus database. In GSE28146, eight control samples, and Alzheimer's disease samples comprising seven incipient, eight moderate, seven severe Alzheimer's disease samples, were included. The Affy package in R was used for background correction and normalization of the raw microarray data. The differentially expressed genes (DEGs) and differentially expressed miRNAs were identified using the Limma package. In addition, mRNAs were clustered using weighted gene correlation network analysis, and modules found to be significantly associated with the stages of Alzheimer's disease were screened out. The Database for Annotation, Visualization, and Integrated Discovery was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. The target genes of the differentially expressed miRNAs were identified using the miRWalk database. Compared with the control samples, 175,59 genes and 90 DEGs were identified in the incipient, moderate and severe Alzheimer's disease samples, respectively. A module, which contained 1,592 genes was found to be closely associated with the stage of Alzheimer's disease and biological processes. In addition, pathways associated with Alzheimer's disease and other neurological diseases were found to be enriched in those genes. A total of 139 overlapped genes were identified between those genes and the DEGs in the three groups. From the miRNA expression profiles, 189 miRNAs were found differentially expressed in the samples from patients with Alzheimer's disease and 1,647 target genes were obtained. In addition, five overlapped genes were identified between those 1,647 target genes and the 139 genes, and these genes may be important pathogenic targets for Alzheimer's disease. Through bioinformatics analysis, potential therapeutic targets and associated biological processes were identified, which may assist in understanding and treatment of Alzheimer's disease.
pubD.A. Spandidos
doi10.3892/mmr.2016.5828
date2016-11