schliessen

Filtern

 

Bibliotheken

TSA increases C/EBP-α expression by increasing its lysine acetylation in hepatic stellate cells

CCAAT enhancer binding protein-α (C/EBP-α) is a transcription factor expressed only in certain tissues, including the liver. It has been previously demonstrated that C/EBP-α may induce apoptosis in hepatic stellate cells (HSCs), raising the question of whether acetylation of C/EBP-α is associated wi... Full description

Journal Title: Molecular Medicine Reports 05/2017, Vol.16(5), pp.6088-6093
Main Author: Tao, Li-Li
Other Authors: Ding, Di , Yin, Wei-Hua , Peng, Ji-Ying , Hou, Chen-Jian , Liu, Xiu-Ping , Chen, Yao-Li
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1791-2997 ; E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2017.7358
Link: http://www.spandidos-publications.com/molecular medicine reports/16/5/6088
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: spandido10.3892/mmr.2017.7358
title: TSA increases C/EBP-α expression by increasing its lysine acetylation in hepatic stellate cells
format: Article
creator:
  • Tao, Li-Li
  • Ding, Di
  • Yin, Wei-Hua
  • Peng, Ji-Ying
  • Hou, Chen-Jian
  • Liu, Xiu-Ping
  • Chen, Yao-Li
subjects:
  • Hepatic Stellate Cells
  • Ccaat Enhancer Binding Protein-Α
  • Apoptosis
  • Acetylation
ispartof: Molecular Medicine Reports, 05/2017, Vol.16(5), pp.6088-6093
description: CCAAT enhancer binding protein-α (C/EBP-α) is a transcription factor expressed only in certain tissues, including the liver. It has been previously demonstrated that C/EBP-α may induce apoptosis in hepatic stellate cells (HSCs), raising the question of whether acetylation of C/EBP-α is associated with HSCs, and the potential associated mechanism. A total of three histone deacetylase inhibitors (HDACIs), including trichostatin A (TSA), suberoylanilide hydroxamic acid and nicotinamide, were selected to determine whether acetylation affects C/EBP-α expression. A Cell Counting Kit-8 assay was used to determine the rate of proliferation inhibition following treatment with varying doses of the three HDACIs in HSC-T6 and BRL-3A cells. Western blot analysis was used to examine Caspase-3, −8, −9, and −12 levels in HSC-T6 cells treated with adenoviral-C/EBP-α and/or TSA. Following treatment with TSA, a combination of reverse transcription-quantitative polymerase chain reaction and western blot analyses was used to determine the inherent C/EBP-α mRNA and protein levels in HSC-T6 cells at 0, 1, 2, 4, 8, 12, 24, 36 and 48 h. Nuclear and cytoplasmic proteins were extracted to examine C/EBP-α distribution. Co-immunoprecipitation analysis was used to examine the lysine acetylation of C/EBP-α. It was observed that TSA inhibited the proliferation of HSC-T6 cells to a greater extent compared with BRL-3A cells, following treatment with the three HDACIs. TSA induced apoptosis in HSC-T6 cells and enhanced the expression of C/EBP-α. Following treatment of HSC-T6 cells with TSA, inherent C/EBP-α expression increased in a time-dependent manner, and its lysine acetylation simultaneously increased. Therefore, the results of the present study suggested that TSA may increase C/EBP-α expression by increasing its lysine acetylation in HSCs.
language: eng
source:
identifier: ISSN: 1791-2997 ; E-ISSN: 1791-3004 ; DOI: 10.3892/mmr.2017.7358
fulltext: fulltext
issn:
  • 1791-2997
  • 17912997
  • 1791-3004
  • 17913004
url: Link


@attributes
ID442697983
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.3892/mmr.2017.7358
sourceidspandido
recordidTN_spandido10.3892/mmr.2017.7358
sourceformatXML
sourcesystemOther
pqid1933601026
galeid525185145
display
typearticle
titleTSA increases C/EBP-α expression by increasing its lysine acetylation in hepatic stellate cells
creatorTao, Li-Li ; Ding, Di ; Yin, Wei-Hua ; Peng, Ji-Ying ; Hou, Chen-Jian ; Liu, Xiu-Ping ; Chen, Yao-Li
ispartofMolecular Medicine Reports, 05/2017, Vol.16(5), pp.6088-6093
identifier
subjectHepatic Stellate Cells ; Ccaat Enhancer Binding Protein-Α ; Apoptosis ; Acetylation
descriptionCCAAT enhancer binding protein-α (C/EBP-α) is a transcription factor expressed only in certain tissues, including the liver. It has been previously demonstrated that C/EBP-α may induce apoptosis in hepatic stellate cells (HSCs), raising the question of whether acetylation of C/EBP-α is associated with HSCs, and the potential associated mechanism. A total of three histone deacetylase inhibitors (HDACIs), including trichostatin A (TSA), suberoylanilide hydroxamic acid and nicotinamide, were selected to determine whether acetylation affects C/EBP-α expression. A Cell Counting Kit-8 assay was used to determine the rate of proliferation inhibition following treatment with varying doses of the three HDACIs in HSC-T6 and BRL-3A cells. Western blot analysis was used to examine Caspase-3, −8, −9, and −12 levels in HSC-T6 cells treated with adenoviral-C/EBP-α and/or TSA. Following treatment with TSA, a combination of reverse transcription-quantitative polymerase chain reaction and western blot analyses was used to determine the inherent C/EBP-α mRNA and protein levels in HSC-T6 cells at 0, 1, 2, 4, 8, 12, 24, 36 and 48 h. Nuclear and cytoplasmic proteins were extracted to examine C/EBP-α distribution. Co-immunoprecipitation analysis was used to examine the lysine acetylation of C/EBP-α. It was observed that TSA inhibited the proliferation of HSC-T6 cells to a greater extent compared with BRL-3A cells, following treatment with the three HDACIs. TSA induced apoptosis in HSC-T6 cells and enhanced the expression of C/EBP-α. Following treatment of HSC-T6 cells with TSA, inherent C/EBP-α expression increased in a time-dependent manner, and its lysine acetylation simultaneously increased. Therefore, the results of the present study suggested that TSA may increase C/EBP-α expression by increasing its lysine acetylation in HSCs.
languageeng
source
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://www.spandidos-publications.com/molecular medicine reports/16/5/6088$$EView_this_record_in_Spandidos_Publications
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Tao, Li-Li
1Ding, Di
2Yin, Wei-Hua
3Peng, Ji-Ying
4Hou, Chen-Jian
5Liu, Xiu-Ping
6Chen, Yao-Li
titleTSA increases C/EBP-α expression by increasing its lysine acetylation in hepatic stellate cells
descriptionCCAAT enhancer binding protein-α (C/EBP-α) is a transcription factor expressed only in certain tissues, including the liver. It has been previously demonstrated that C/EBP-α may induce apoptosis in hepatic stellate cells (HSCs), raising the question of whether acetylation of C/EBP-α is associated with HSCs, and the potential associated mechanism. A total of three histone deacetylase inhibitors (HDACIs), including trichostatin A (TSA), suberoylanilide hydroxamic acid and nicotinamide, were selected to determine whether acetylation affects C/EBP-α expression. A Cell Counting Kit-8 assay was used to determine the rate of proliferation inhibition following treatment with varying doses of the three HDACIs in HSC-T6 and BRL-3A cells. Western blot analysis was used to examine Caspase-3, −8, −9, and −12 levels in HSC-T6 cells treated with adenoviral-C/EBP-α and/or TSA. Following treatment with TSA, a combination of reverse transcription-quantitative polymerase chain reaction and western blot analyses was used to determine the inherent C/EBP-α mRNA and protein levels in HSC-T6 cells at 0, 1, 2, 4, 8, 12, 24, 36 and 48 h. Nuclear and cytoplasmic proteins were extracted to examine C/EBP-α distribution. Co-immunoprecipitation analysis was used to examine the lysine acetylation of C/EBP-α. It was observed that TSA inhibited the proliferation of HSC-T6 cells to a greater extent compared with BRL-3A cells, following treatment with the three HDACIs. TSA induced apoptosis in HSC-T6 cells and enhanced the expression of C/EBP-α. Following treatment of HSC-T6 cells with TSA, inherent C/EBP-α expression increased in a time-dependent manner, and its lysine acetylation simultaneously increased. Therefore, the results of the present study suggested that TSA may increase C/EBP-α expression by increasing its lysine acetylation in HSCs.
subject
0hepatic stellate cells
1CCAAT enhancer binding protein-α
2apoptosis
3acetylation
general
010.3892/mmr.2017.7358
1D.A. Spandidos
2English
3Spandidios Publications (Spandidos Publications Ltd.)
sourceidspandido
recordidspandido10.3892/mmr.2017.7358
issn
01791-2997
117912997
21791-3004
317913004
rsrctypearticle
creationdate2017
addtitleMolecular Medicine Reports
searchscopespandidos
scopespandidos
lsr30VSR-Enriched:[pqid, galeid]
sort
titleTSA increases C/EBP-α expression by increasing its lysine acetylation in hepatic stellate cells
authorTao, Li-Li ; Ding, Di ; Yin, Wei-Hua ; Peng, Ji-Ying ; Hou, Chen-Jian ; Liu, Xiu-Ping ; Chen, Yao-Li
creationdate20170500
lso0120170500
facets
frbrgroupid4691492970845093847
frbrtype5
newrecords20180110
languageeng
creationdate2017
topic
0hepatic stellate cells
1CCAAT enhancer binding protein-α
2apoptosis
3acetylation
collectionSpandidios Publications (Spandidos Publications Ltd.)
prefilterarticles
rsrctypearticles
creatorcontrib
0Tao, Li-Li
1Ding, Di
2Yin, Wei-Hua
3Peng, Ji-Ying
4Hou, Chen-Jian
5Liu, Xiu-Ping
6Chen, Yao-Li
jtitleMolecular Medicine Reports
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Tao
1Ding
2Yin
3Peng
4Hou
5Liu
6Chen
aufirst
0Li-Li
1Di
2Wei-Hua
3Ji-Ying
4Chen-Jian
5Xiu-Ping
6Yao-Li
au
0Tao, Li-Li
1Ding, Di
2Yin, Wei-Hua
3Peng, Ji-Ying
4Hou, Chen-Jian
5Liu, Xiu-Ping
6Chen, Yao-Li
atitleTSA increases C/EBP-α expression by increasing its lysine acetylation in hepatic stellate cells
jtitleMolecular Medicine Reports
risdate201705
volume16
issue5
spage6088
epage6093
pages6088-6093
issn1791-2997
eissn1791-3004
formatjournal
genrearticle
ristypeJOUR
abstractCCAAT enhancer binding protein-α (C/EBP-α) is a transcription factor expressed only in certain tissues, including the liver. It has been previously demonstrated that C/EBP-α may induce apoptosis in hepatic stellate cells (HSCs), raising the question of whether acetylation of C/EBP-α is associated with HSCs, and the potential associated mechanism. A total of three histone deacetylase inhibitors (HDACIs), including trichostatin A (TSA), suberoylanilide hydroxamic acid and nicotinamide, were selected to determine whether acetylation affects C/EBP-α expression. A Cell Counting Kit-8 assay was used to determine the rate of proliferation inhibition following treatment with varying doses of the three HDACIs in HSC-T6 and BRL-3A cells. Western blot analysis was used to examine Caspase-3, −8, −9, and −12 levels in HSC-T6 cells treated with adenoviral-C/EBP-α and/or TSA. Following treatment with TSA, a combination of reverse transcription-quantitative polymerase chain reaction and western blot analyses was used to determine the inherent C/EBP-α mRNA and protein levels in HSC-T6 cells at 0, 1, 2, 4, 8, 12, 24, 36 and 48 h. Nuclear and cytoplasmic proteins were extracted to examine C/EBP-α distribution. Co-immunoprecipitation analysis was used to examine the lysine acetylation of C/EBP-α. It was observed that TSA inhibited the proliferation of HSC-T6 cells to a greater extent compared with BRL-3A cells, following treatment with the three HDACIs. TSA induced apoptosis in HSC-T6 cells and enhanced the expression of C/EBP-α. Following treatment of HSC-T6 cells with TSA, inherent C/EBP-α expression increased in a time-dependent manner, and its lysine acetylation simultaneously increased. Therefore, the results of the present study suggested that TSA may increase C/EBP-α expression by increasing its lysine acetylation in HSCs.
pubD.A. Spandidos
doi10.3892/mmr.2017.7358
date2017-05