schliessen

Filtern

 

Bibliotheken

Protein S100-A8: A potential metastasis-associated protein for breast cancer determined via iTRAQ quantitative proteomic and clinicopathological analysis

Breast cancer is the most common malignancy in females, with metastasis of this type of cancer frequently proving lethal. However, there are still no effective biomarkers to predict breast cancer metastasis. The aim of the present study was, therefore, to analyze breast cancer metastasis-associated... Full description

Journal Title: Oncology Letters 04/2018, Vol.15(4), pp.5285-5293
Main Author: Zhong, Jing-Min
Other Authors: Li, Jing , Kang, An-Ding , Huang, San-Qian , Liu, Wen-Bin , Zhang, Yun , Liu, Zhi-Hong , Zeng, Liang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1792-1074 ; E-ISSN: 1792-1082 ; DOI: 10.3892/ol.2018.7958
Link: http://www.spandidos-publications.com/ol/15/4/5285
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: spandido10.3892/ol.2018.7958
title: Protein S100-A8: A potential metastasis-associated protein for breast cancer determined via iTRAQ quantitative proteomic and clinicopathological analysis
format: Article
creator:
  • Zhong, Jing-Min
  • Li, Jing
  • Kang, An-Ding
  • Huang, San-Qian
  • Liu, Wen-Bin
  • Zhang, Yun
  • Liu, Zhi-Hong
  • Zeng, Liang
subjects:
  • Breast Neoplasm
  • Neoplasm Metastasis
  • Prognosis
  • Isobaric Tags For Relative And Absolute Quantitation
  • Protein S100-A8
ispartof: Oncology Letters, 04/2018, Vol.15(4), pp.5285-5293
description: Breast cancer is the most common malignancy in females, with metastasis of this type of cancer frequently proving lethal. However, there are still no effective biomarkers to predict breast cancer metastasis. The aim of the present study was, therefore, to analyze breast cancer metastasis-associated proteins and evaluate the association between protein S100-A8 and the prognosis of breast cancer. The isobaric tags for relative and absolute quantitation (iTRAQ) proteomic technique was used to analyze the differential expression of proteins between fresh primary breast tumor (PBT) tissue and fresh paired metastatic lymph nodes (PMLN) tissue. Subsequently, immunohistochemical staining was used to locate and assess the expression of protein S100-A8 in benign breast disease (n=15), primary breast cancer with (n=109) or without (n=83) metastasis, and in paired metastatic lymph nodes (n=109) formalin fixed paraffin embedded (FFPE) tissue. Staining scores were evaluated and the association between protein S100-A8 expression levels and the clinicopathological characteristics of 192 patients with breast cancer were evaluated using the χ 2 test. Kaplan-Meier and Cox hazards regression analyses were utilized to investigate the association between the expression of protein S100-A8 and the prognosis of patients with breast cancer. A total of 4,837 proteins were identified using the iTRAQ proteomic technique. Among these proteins, 643 differentially expressed proteins were revealed. Protein S100-A8 expression levels were identified to differ between PBT and PMLN tissues. Immunohistochemical staining suggested a significant difference between NMBT and PMLN (P=0.002), and also between PBT and PMLN (P
language: eng
source:
identifier: ISSN: 1792-1074 ; E-ISSN: 1792-1082 ; DOI: 10.3892/ol.2018.7958
fulltext: fulltext
issn:
  • 1792-1074
  • 17921074
  • 1792-1082
  • 17921082
url: Link


@attributes
ID905603287
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.3892/ol.2018.7958
sourceidspandido
recordidTN_spandido10.3892/ol.2018.7958
sourceformatXML
sourcesystemPC
pqid2020420758
galeid535030869
display
typearticle
titleProtein S100-A8: A potential metastasis-associated protein for breast cancer determined via iTRAQ quantitative proteomic and clinicopathological analysis
creatorZhong, Jing-Min ; Li, Jing ; Kang, An-Ding ; Huang, San-Qian ; Liu, Wen-Bin ; Zhang, Yun ; Liu, Zhi-Hong ; Zeng, Liang
ispartofOncology Letters, 04/2018, Vol.15(4), pp.5285-5293
identifier
subjectBreast Neoplasm ; Neoplasm Metastasis ; Prognosis ; Isobaric Tags For Relative And Absolute Quantitation ; Protein S100-A8
descriptionBreast cancer is the most common malignancy in females, with metastasis of this type of cancer frequently proving lethal. However, there are still no effective biomarkers to predict breast cancer metastasis. The aim of the present study was, therefore, to analyze breast cancer metastasis-associated proteins and evaluate the association between protein S100-A8 and the prognosis of breast cancer. The isobaric tags for relative and absolute quantitation (iTRAQ) proteomic technique was used to analyze the differential expression of proteins between fresh primary breast tumor (PBT) tissue and fresh paired metastatic lymph nodes (PMLN) tissue. Subsequently, immunohistochemical staining was used to locate and assess the expression of protein S100-A8 in benign breast disease (n=15), primary breast cancer with (n=109) or without (n=83) metastasis, and in paired metastatic lymph nodes (n=109) formalin fixed paraffin embedded (FFPE) tissue. Staining scores were evaluated and the association between protein S100-A8 expression levels and the clinicopathological characteristics of 192 patients with breast cancer were evaluated using the χ 2 test. Kaplan-Meier and Cox hazards regression analyses were utilized to investigate the association between the expression of protein S100-A8 and the prognosis of patients with breast cancer. A total of 4,837 proteins were identified using the iTRAQ proteomic technique. Among these proteins, 643 differentially expressed proteins were revealed. Protein S100-A8 expression levels were identified to differ between PBT and PMLN tissues. Immunohistochemical staining suggested a significant difference between NMBT and PMLN (P=0.002), and also between PBT and PMLN (P<0.001). Cox hazards regression model analyses suggested that histological grade (P=0.031) and nodal status (P=0.001) were risk factors for lymph nodes metastasis of breast cancer. Kaplan-Meier analyses revealed no significant relationship between protein S100-A8 expression level and overall survival rate of patients with breast cancer. In conclusion, by using the iTRAQ proteomic technique and immunohistochemistry staining, it was identified that protein S100-A8 may be associated with lymph nodes metastasis of breast cancer and be a marker for progression of breast cancer.
languageeng
source
version6
lds50peer_reviewed
links
openurl$$Topenurl_article
backlink$$Uhttp://www.spandidos-publications.com/ol/15/4/5285$$EView_this_record_in_Spandidos_Publications
openurlfulltext$$Topenurlfull_article
search
creatorcontrib
0Zhong, Jing-Min
1Li, Jing
2Kang, An-Ding
3Huang, San-Qian
4Liu, Wen-Bin
5Zhang, Yun
6Liu, Zhi-Hong
7Zeng, Liang
titleProtein S100-A8: A potential metastasis-associated protein for breast cancer determined via iTRAQ quantitative proteomic and clinicopathological analysis
descriptionBreast cancer is the most common malignancy in females, with metastasis of this type of cancer frequently proving lethal. However, there are still no effective biomarkers to predict breast cancer metastasis. The aim of the present study was, therefore, to analyze breast cancer metastasis-associated proteins and evaluate the association between protein S100-A8 and the prognosis of breast cancer. The isobaric tags for relative and absolute quantitation (iTRAQ) proteomic technique was used to analyze the differential expression of proteins between fresh primary breast tumor (PBT) tissue and fresh paired metastatic lymph nodes (PMLN) tissue. Subsequently, immunohistochemical staining was used to locate and assess the expression of protein S100-A8 in benign breast disease (n=15), primary breast cancer with (n=109) or without (n=83) metastasis, and in paired metastatic lymph nodes (n=109) formalin fixed paraffin embedded (FFPE) tissue. Staining scores were evaluated and the association between protein S100-A8 expression levels and the clinicopathological characteristics of 192 patients with breast cancer were evaluated using the χ 2 test. Kaplan-Meier and Cox hazards regression analyses were utilized to investigate the association between the expression of protein S100-A8 and the prognosis of patients with breast cancer. A total of 4,837 proteins were identified using the iTRAQ proteomic technique. Among these proteins, 643 differentially expressed proteins were revealed. Protein S100-A8 expression levels were identified to differ between PBT and PMLN tissues. Immunohistochemical staining suggested a significant difference between NMBT and PMLN (P=0.002), and also between PBT and PMLN (P<0.001). Cox hazards regression model analyses suggested that histological grade (P=0.031) and nodal status (P=0.001) were risk factors for lymph nodes metastasis of breast cancer. Kaplan-Meier analyses revealed no significant relationship between protein S100-A8 expression level and overall survival rate of patients with breast cancer. In conclusion, by using the iTRAQ proteomic technique and immunohistochemistry staining, it was identified that protein S100-A8 may be associated with lymph nodes metastasis of breast cancer and be a marker for progression of breast cancer.
subject
0breast neoplasm
1neoplasm metastasis
2prognosis
3isobaric tags for relative and absolute quantitation
4protein S100-A8
general
010.3892/ol.2018.7958
1D.A. Spandidos
2English
3Spandidios Publications (Spandidos Publications Ltd.)
sourceidspandido
recordidspandido10.3892/ol.2018.7958
issn
01792-1074
117921074
21792-1082
317921082
rsrctypearticle
creationdate2018
addtitleOncology Letters
searchscopespandidos
scopespandidos
lsr30VSR-Enriched:[galeid, pqid]
sort
titleProtein S100-A8: A potential metastasis-associated protein for breast cancer determined via iTRAQ quantitative proteomic and clinicopathological analysis
authorZhong, Jing-Min ; Li, Jing ; Kang, An-Ding ; Huang, San-Qian ; Liu, Wen-Bin ; Zhang, Yun ; Liu, Zhi-Hong ; Zeng, Liang
creationdate20180400
lso0120180400
facets
frbrgroupid7773535139192809834
frbrtype5
newrecords20180404
languageeng
creationdate2018
topic
0breast neoplasm
1neoplasm metastasis
2prognosis
3isobaric tags for relative and absolute quantitation
4protein S100-A8
collectionSpandidios Publications (Spandidos Publications Ltd.)
prefilterarticles
rsrctypearticles
creatorcontrib
0Zhong, Jing-Min
1Li, Jing
2Kang, An-Ding
3Huang, San-Qian
4Liu, Wen-Bin
5Zhang, Yun
6Liu, Zhi-Hong
7Zeng, Liang
jtitleOncology Letters
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Zhong
1Li
2Kang
3Huang
4Liu
5Zhang
6Zeng
aufirst
0Jing-Min
1Jing
2An-Ding
3San-Qian
4Wen-Bin
5Yun
6Zhi-Hong
7Liang
au
0Zhong, Jing-Min
1Li, Jing
2Kang, An-Ding
3Huang, San-Qian
4Liu, Wen-Bin
5Zhang, Yun
6Liu, Zhi-Hong
7Zeng, Liang
atitleProtein S100-A8: A potential metastasis-associated protein for breast cancer determined via iTRAQ quantitative proteomic and clinicopathological analysis
jtitleOncology Letters
risdate201804
volume15
issue4
spage5285
epage5293
pages5285-5293
issn1792-1074
eissn1792-1082
formatjournal
genrearticle
ristypeJOUR
abstractBreast cancer is the most common malignancy in females, with metastasis of this type of cancer frequently proving lethal. However, there are still no effective biomarkers to predict breast cancer metastasis. The aim of the present study was, therefore, to analyze breast cancer metastasis-associated proteins and evaluate the association between protein S100-A8 and the prognosis of breast cancer. The isobaric tags for relative and absolute quantitation (iTRAQ) proteomic technique was used to analyze the differential expression of proteins between fresh primary breast tumor (PBT) tissue and fresh paired metastatic lymph nodes (PMLN) tissue. Subsequently, immunohistochemical staining was used to locate and assess the expression of protein S100-A8 in benign breast disease (n=15), primary breast cancer with (n=109) or without (n=83) metastasis, and in paired metastatic lymph nodes (n=109) formalin fixed paraffin embedded (FFPE) tissue. Staining scores were evaluated and the association between protein S100-A8 expression levels and the clinicopathological characteristics of 192 patients with breast cancer were evaluated using the χ 2 test. Kaplan-Meier and Cox hazards regression analyses were utilized to investigate the association between the expression of protein S100-A8 and the prognosis of patients with breast cancer. A total of 4,837 proteins were identified using the iTRAQ proteomic technique. Among these proteins, 643 differentially expressed proteins were revealed. Protein S100-A8 expression levels were identified to differ between PBT and PMLN tissues. Immunohistochemical staining suggested a significant difference between NMBT and PMLN (P=0.002), and also between PBT and PMLN (P<0.001). Cox hazards regression model analyses suggested that histological grade (P=0.031) and nodal status (P=0.001) were risk factors for lymph nodes metastasis of breast cancer. Kaplan-Meier analyses revealed no significant relationship between protein S100-A8 expression level and overall survival rate of patients with breast cancer. In conclusion, by using the iTRAQ proteomic technique and immunohistochemistry staining, it was identified that protein S100-A8 may be associated with lymph nodes metastasis of breast cancer and be a marker for progression of breast cancer.
pubD.A. Spandidos
doi10.3892/ol.2018.7958
date2018-04