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Suv4-20h1 promotes G1 to S phase transition by downregulating p21 WAF1/CIP1 expression in chronic myeloid leukemia K562 cells

Methylation of histone H4 lysine 20 (H4K20) has been associated with cancer. However, the functions of the histone methyltransferases that trigger histone H4K20 methylation in cancers, including suppressor of variegation 4–20 homolog 1 (Suv4-20h1), remain elusive. In the present study, it was demons... Full description

Journal Title: Oncology Letters 05/2018, Vol.15(5), pp.6123-6130
Main Author: Wu, Yupeng
Other Authors: Wang, Yadong , Liu, Ming , Nie, Min , Wang, Ying , Deng, Yexuan , Yao, Bing , Gui, Tao , Li, Xinyu , Ma, Lingling , Guo, Chan , Ma, Chi , Ju, Junyi , Zhao, Quan
Format: Electronic Article Electronic Article
Language: English
Subjects:
P21
ID: ISSN: 1792-1074 ; E-ISSN: 1792-1082 ; DOI: 10.3892/ol.2018.8092
Link: http://www.spandidos-publications.com/ol/15/5/6123
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recordid: spandido10.3892/ol.2018.8092
title: Suv4-20h1 promotes G1 to S phase transition by downregulating p21 WAF1/CIP1 expression in chronic myeloid leukemia K562 cells
format: Article
creator:
  • Wu, Yupeng
  • Wang, Yadong
  • Liu, Ming
  • Nie, Min
  • Wang, Ying
  • Deng, Yexuan
  • Yao, Bing
  • Gui, Tao
  • Li, Xinyu
  • Ma, Lingling
  • Guo, Chan
  • Ma, Chi
  • Ju, Junyi
  • Zhao, Quan
subjects:
  • Suv4-20h1
  • P21
  • Cell Cycle
  • K562
  • Histone Methylation
ispartof: Oncology Letters, 05/2018, Vol.15(5), pp.6123-6130
description: Methylation of histone H4 lysine 20 (H4K20) has been associated with cancer. However, the functions of the histone methyltransferases that trigger histone H4K20 methylation in cancers, including suppressor of variegation 4–20 homolog 1 (Suv4-20h1), remain elusive. In the present study, it was demonstrated that the knockdown of the histone H4K20 methyltransferase Suv4-20h1 resulted in growth inhibition in chronic myeloid leukemia K562 cells. Disruption of Suv4-20h1 expression induced G 1 arrest in the cell cycle and increased expression levels of cyclin dependent kinase inhibitor 1A (p21 WAF1/CIP1 ), an essential cell cycle protein involved in checkpoint regulation. Chromatin immunoprecipitation analysis demonstrated that Suv4-20h1 directly binds to the promoter of the p21 gene and that methylation of histone H4K20 correlates with repression of p21 expression. Thus, these data suggest that Suv4-20h1 is important for the regulation of the cell cycle in K562 cells and may be a potential therapeutic target for leukemia.
language: eng
source:
identifier: ISSN: 1792-1074 ; E-ISSN: 1792-1082 ; DOI: 10.3892/ol.2018.8092
fulltext: fulltext
issn:
  • 1792-1074
  • 17921074
  • 1792-1082
  • 17921082
url: Link


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titleSuv4-20h1 promotes G1 to S phase transition by downregulating p21 WAF1/CIP1 expression in chronic myeloid leukemia K562 cells
creatorWu, Yupeng ; Wang, Yadong ; Liu, Ming ; Nie, Min ; Wang, Ying ; Deng, Yexuan ; Yao, Bing ; Gui, Tao ; Li, Xinyu ; Ma, Lingling ; Guo, Chan ; Ma, Chi ; Ju, Junyi ; Zhao, Quan
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subjectSuv4-20h1 ; P21 ; Cell Cycle ; K562 ; Histone Methylation
descriptionMethylation of histone H4 lysine 20 (H4K20) has been associated with cancer. However, the functions of the histone methyltransferases that trigger histone H4K20 methylation in cancers, including suppressor of variegation 4–20 homolog 1 (Suv4-20h1), remain elusive. In the present study, it was demonstrated that the knockdown of the histone H4K20 methyltransferase Suv4-20h1 resulted in growth inhibition in chronic myeloid leukemia K562 cells. Disruption of Suv4-20h1 expression induced G 1 arrest in the cell cycle and increased expression levels of cyclin dependent kinase inhibitor 1A (p21 WAF1/CIP1 ), an essential cell cycle protein involved in checkpoint regulation. Chromatin immunoprecipitation analysis demonstrated that Suv4-20h1 directly binds to the promoter of the p21 gene and that methylation of histone H4K20 correlates with repression of p21 expression. Thus, these data suggest that Suv4-20h1 is important for the regulation of the cell cycle in K562 cells and may be a potential therapeutic target for leukemia.
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titleSuv4-20h1 promotes G1 to S phase transition by downregulating p21 WAF1/CIP1 expression in chronic myeloid leukemia K562 cells
descriptionMethylation of histone H4 lysine 20 (H4K20) has been associated with cancer. However, the functions of the histone methyltransferases that trigger histone H4K20 methylation in cancers, including suppressor of variegation 4–20 homolog 1 (Suv4-20h1), remain elusive. In the present study, it was demonstrated that the knockdown of the histone H4K20 methyltransferase Suv4-20h1 resulted in growth inhibition in chronic myeloid leukemia K562 cells. Disruption of Suv4-20h1 expression induced G 1 arrest in the cell cycle and increased expression levels of cyclin dependent kinase inhibitor 1A (p21 WAF1/CIP1 ), an essential cell cycle protein involved in checkpoint regulation. Chromatin immunoprecipitation analysis demonstrated that Suv4-20h1 directly binds to the promoter of the p21 gene and that methylation of histone H4K20 correlates with repression of p21 expression. Thus, these data suggest that Suv4-20h1 is important for the regulation of the cell cycle in K562 cells and may be a potential therapeutic target for leukemia.
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abstractMethylation of histone H4 lysine 20 (H4K20) has been associated with cancer. However, the functions of the histone methyltransferases that trigger histone H4K20 methylation in cancers, including suppressor of variegation 4–20 homolog 1 (Suv4-20h1), remain elusive. In the present study, it was demonstrated that the knockdown of the histone H4K20 methyltransferase Suv4-20h1 resulted in growth inhibition in chronic myeloid leukemia K562 cells. Disruption of Suv4-20h1 expression induced G 1 arrest in the cell cycle and increased expression levels of cyclin dependent kinase inhibitor 1A (p21 WAF1/CIP1 ), an essential cell cycle protein involved in checkpoint regulation. Chromatin immunoprecipitation analysis demonstrated that Suv4-20h1 directly binds to the promoter of the p21 gene and that methylation of histone H4K20 correlates with repression of p21 expression. Thus, these data suggest that Suv4-20h1 is important for the regulation of the cell cycle in K562 cells and may be a potential therapeutic target for leukemia.
pubD.A. Spandidos
doi10.3892/ol.2018.8092
date2018-05