schliessen

Filtern

 

Bibliotheken

Induction of muscle weakness by local inflammation: an experimental animal model

Byline: S. Bicer (1), P. J. Reiser (1), S. Ching (1), N. Quan (1) Keywords: Myosin heavy chain; Isoform; Skeletal muscle; Force Abstract: Objective and design: The objective of this study was to characterize the response of skeletal muscle to a localized inflammation induced by the inflammatory agen... Full description

Journal Title: Inflammation Research 2009, Vol.58(4), pp.175-183
Main Author: Bicer, S.
Other Authors: Reiser, P. , Ching, S. , Quan, N.
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1023-3830 ; E-ISSN: 1420-908X ; DOI: 10.1007/s00011-008-8093-7
Link: http://dx.doi.org/10.1007/s00011-008-8093-7
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: springer_jour10.1007/s00011-008-8093-7
title: Induction of muscle weakness by local inflammation: an experimental animal model
format: Article
creator:
  • Bicer, S.
  • Reiser, P.
  • Ching, S.
  • Quan, N.
subjects:
  • Myosin heavy chain
  • Isoform
  • Skeletal muscle
  • Force
ispartof: Inflammation Research, 2009, Vol.58(4), pp.175-183
description: Byline: S. Bicer (1), P. J. Reiser (1), S. Ching (1), N. Quan (1) Keywords: Myosin heavy chain; Isoform; Skeletal muscle; Force Abstract: Objective and design: The objective of this study was to characterize the response of skeletal muscle to a localized inflammation induced by the inflammatory agent casein. Methods: An inflammatory agent, casein, was injected into the right hindlimb and saline was injected into the left hindlimb of normal adult mice, once daily for six consecutive days. Inflammatory response was monitored by immunohistochemical labeling of leukocytes. Muscle protein levels were determined by electrophoresis and muscle function was determined by isometric force measurements. Results: Local inflammation was induced by casein in association with the accumulation of extensive neutrophils and macrophages in the solues muscle. This local inflammation resulted in a shift in myosin heavy chain (MHC) isoform expression and a significant reduction in total MHC concentration in the soleus. Maximal twitch and tetanic forces were significantly reduced in the inflamed soleus. Contractile function in soleus was fully restored after two weeks of recovery, along with the restoration of protein concentration and the disappearance of inflammatory cells. Conclusion: This study establishes a unique and robust model in which mechanisms of local inflammation induced muscle protein degradation, reduction of contractile force, and subsequent recovery from this condition can be further studied. Author Affiliation: (1) Department of Oral Biology, Ohio State University, Columbus, OH, 43210-1247, USA Article History: Registration Date: 01/01/2008 Online Date: 11/02/2009 Article note: Received 22 February 2008 returned for revision 17 April 2008 received from final revision 2 July 2008 accepted by M. Parnham 7 July 2008
language: eng
source:
identifier: ISSN: 1023-3830 ; E-ISSN: 1420-908X ; DOI: 10.1007/s00011-008-8093-7
fulltext: fulltext
issn:
  • 1420-908X
  • 1420908X
  • 1023-3830
  • 10233830
url: Link


@attributes
ID978849101
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid10.1007/s00011-008-8093-7
sourceidspringer_jour
recordidTN_springer_jour10.1007/s00011-008-8093-7
sourcesystemOther
pqid67046366
galeid232746806
display
typearticle
titleInduction of muscle weakness by local inflammation: an experimental animal model
creatorBicer, S. ; Reiser, P. ; Ching, S. ; Quan, N.
ispartofInflammation Research, 2009, Vol.58(4), pp.175-183
identifier
subjectMyosin heavy chain ; Isoform ; Skeletal muscle ; Force
languageeng
source
descriptionByline: S. Bicer (1), P. J. Reiser (1), S. Ching (1), N. Quan (1) Keywords: Myosin heavy chain; Isoform; Skeletal muscle; Force Abstract: Objective and design: The objective of this study was to characterize the response of skeletal muscle to a localized inflammation induced by the inflammatory agent casein. Methods: An inflammatory agent, casein, was injected into the right hindlimb and saline was injected into the left hindlimb of normal adult mice, once daily for six consecutive days. Inflammatory response was monitored by immunohistochemical labeling of leukocytes. Muscle protein levels were determined by electrophoresis and muscle function was determined by isometric force measurements. Results: Local inflammation was induced by casein in association with the accumulation of extensive neutrophils and macrophages in the solues muscle. This local inflammation resulted in a shift in myosin heavy chain (MHC) isoform expression and a significant reduction in total MHC concentration in the soleus. Maximal twitch and tetanic forces were significantly reduced in the inflamed soleus. Contractile function in soleus was fully restored after two weeks of recovery, along with the restoration of protein concentration and the disappearance of inflammatory cells. Conclusion: This study establishes a unique and robust model in which mechanisms of local inflammation induced muscle protein degradation, reduction of contractile force, and subsequent recovery from this condition can be further studied. Author Affiliation: (1) Department of Oral Biology, Ohio State University, Columbus, OH, 43210-1247, USA Article History: Registration Date: 01/01/2008 Online Date: 11/02/2009 Article note: Received 22 February 2008 returned for revision 17 April 2008 received from final revision 2 July 2008 accepted by M. Parnham 7 July 2008
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://dx.doi.org/10.1007/s00011-008-8093-7$$EView_full_text_in_Springer_(Subscribers_only)
search
creatorcontrib
0Bicer, S., J.
1Reiser, P., J.
2Ching, S., J.
3Quan, N., J.
titleInduction of muscle weakness by local inflammation: an experimental animal model
subject
0Myosin heavy chain
1Isoform
2Skeletal muscle
3Force
general
010.1007/s00011-008-8093-7
1English
2Springer Science & Business Media B.V.
3SpringerLink
sourceidspringer_jour
recordidspringer_jour10.1007/s00011-008-8093-7
issn
01420-908X
11420908X
21023-3830
310233830
rsrctypearticle
creationdate2009
addtitle
0Inflammation Research
1Official Journal of: The International Association of Inflammation Societies The European Histamine Research Society
2Inflamm. Res.
searchscopespringer_journals_complete
scopespringer_journals_complete
lsr30VSR-Enriched:[pqid, pages, description, galeid]
sort
titleInduction of muscle weakness by local inflammation: an experimental animal model
authorBicer, S. ; Reiser, P. ; Ching, S. ; Quan, N.
creationdate20090400
facets
frbrgroupid3845165692436726970
frbrtype5
languageeng
creationdate2009
topic
0Myosin Heavy Chain
1Isoform
2Skeletal Muscle
3Force
collectionSpringerLink
prefilterarticles
rsrctypearticles
creatorcontrib
0Bicer, S.
1Reiser, P.
2Ching, S.
3Quan, N.
jtitleInflammation Research
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Bicer
1Reiser
2Ching
3Quan
aufirst
0S.
1P.
2J.
3N.
au
0Bicer, S.
1Reiser, P.
2Ching, S.
3Quan, N.
atitleInduction of muscle weakness by local inflammation: an experimental animal model
jtitleInflammation Research
stitleInflamm. Res.
addtitleOfficial Journal of: The International Association of Inflammation Societies The European Histamine Research Society
risdate200904
volume58
issue4
spage175
epage183
issn1023-3830
eissn1420-908X
genrearticle
ristypeJOUR
copBasel
pubBirkhäuser-Verlag
doi10.1007/s00011-008-8093-7
pages175-17583
date2009-04