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Contribution of Orthodontic Load to Inflammationmediated Periodontal Destruction

Byline: Marjan Nokhbehsaim (1,2), Birgit Deschner (1,3), Jochen Winter (2), Susanne Reimann (4), Christoph Bourauel (1,4), Soren Jepsen (1,2), Andreas Jager (1,3), James Deschner (1,2,5) Keywords: Orthodontic load; Biomechanical forces; Inflammation; Periodontitis; Periodontium; PDL cells; Cytokines... Full description

Journal Title: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie 2010, Vol.71(6), pp.390-402
Main Author: Nokhbehsaim, Marjan
Other Authors: Deschner, Birgit , Winter, Jochen , Reimann, Susanne , Bourauel, Christoph , Jepsen, Søren , Jäger, Andreas , Deschner, James
Format: Electronic Article Electronic Article
Language: eng ; ger
Subjects:
ID: ISSN: 1434-5293 ; E-ISSN: 1615-6714 ; DOI: 10.1007/s00056-010-1031-7
Link: http://dx.doi.org/10.1007/s00056-010-1031-7
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recordid: springer_jour10.1007/s00056-010-1031-7
title: Contribution of Orthodontic Load to Inflammationmediated Periodontal Destruction
format: Article
creator:
  • Nokhbehsaim, Marjan
  • Deschner, Birgit
  • Winter, Jochen
  • Reimann, Susanne
  • Bourauel, Christoph
  • Jepsen, Søren
  • Jäger, Andreas
  • Deschner, James
subjects:
  • Orthodontic load
  • Biomechanical forces
  • Inflammation
  • Periodontitis
  • Periodontium
  • PDL cells
  • Cytokines
ispartof: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie, 2010, Vol.71(6), pp.390-402
description: Byline: Marjan Nokhbehsaim (1,2), Birgit Deschner (1,3), Jochen Winter (2), Susanne Reimann (4), Christoph Bourauel (1,4), Soren Jepsen (1,2), Andreas Jager (1,3), James Deschner (1,2,5) Keywords: Orthodontic load; Biomechanical forces; Inflammation; Periodontitis; Periodontium; PDL cells; Cytokines; Kieferorthopadische Belastung; Biomechanische Krafte; Entzundung; Parodontitis; Parodont; PDL-Zellen; Zytokine Abstract: Abstract Aim: Orthodontic malpractice as well as hyperocclusal forces can aggravate periodontitis-induced destruction of tooth-supporting tissues, but the underlying mechanisms for the co-destructive effect of biomechanical loading are yet to be elucidated. This in-vitro study was performed to investigate whether biomechanical forces modulate the response of periodontal ligament (PDL) cells to inflammation. Materials and Methods: PDL cells (from six donors) grown on BioFlex.sup.(r) plates were treated with interleukin (IL) 1, which is increased at inflamed periodontal sites, and/or subjected to cyclic tensile strain (CTS) of low (3%) and high (20%) magnitudes for 1[beta] and 6 d. The synthesis of proinflammatory mediators (IL1[beta], IL8, COX2), growth factors (IGF1, VEGF, TGF[beta]1), collagen type 1 (COL1) and osteogenic proteins (ALP, RUNX2) was analyzed by real-time PCR and ELISA. The wound fill rate was examined in an in-vitro wound healing assay. For statistical analyses, Student's t-test and ANOVA were applied (p
language: eng ; ger
source:
identifier: ISSN: 1434-5293 ; E-ISSN: 1615-6714 ; DOI: 10.1007/s00056-010-1031-7
fulltext: fulltext
issn:
  • 1615-6714
  • 16156714
  • 1434-5293
  • 14345293
url: Link


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titleContribution of Orthodontic Load to Inflammationmediated Periodontal Destruction
creatorNokhbehsaim, Marjan ; Deschner, Birgit ; Winter, Jochen ; Reimann, Susanne ; Bourauel, Christoph ; Jepsen, Søren ; Jäger, Andreas ; Deschner, James
ispartofJournal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie, 2010, Vol.71(6), pp.390-402
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subjectOrthodontic load ; Biomechanical forces ; Inflammation ; Periodontitis ; Periodontium ; PDL cells ; Cytokines
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vertitleBeteiligung von kieferorthopädischer Belastung an der entzündungsvermittelten Destruktion des Parodontiums
unititleBeteiligung von kieferorthopädischer Belastung an der entzündungsvermittelten Destruktion des Parodontiums
descriptionByline: Marjan Nokhbehsaim (1,2), Birgit Deschner (1,3), Jochen Winter (2), Susanne Reimann (4), Christoph Bourauel (1,4), Soren Jepsen (1,2), Andreas Jager (1,3), James Deschner (1,2,5) Keywords: Orthodontic load; Biomechanical forces; Inflammation; Periodontitis; Periodontium; PDL cells; Cytokines; Kieferorthopadische Belastung; Biomechanische Krafte; Entzundung; Parodontitis; Parodont; PDL-Zellen; Zytokine Abstract: Abstract Aim: Orthodontic malpractice as well as hyperocclusal forces can aggravate periodontitis-induced destruction of tooth-supporting tissues, but the underlying mechanisms for the co-destructive effect of biomechanical loading are yet to be elucidated. This in-vitro study was performed to investigate whether biomechanical forces modulate the response of periodontal ligament (PDL) cells to inflammation. Materials and Methods: PDL cells (from six donors) grown on BioFlex.sup.(r) plates were treated with interleukin (IL) 1, which is increased at inflamed periodontal sites, and/or subjected to cyclic tensile strain (CTS) of low (3%) and high (20%) magnitudes for 1[beta] and 6 d. The synthesis of proinflammatory mediators (IL1[beta], IL8, COX2), growth factors (IGF1, VEGF, TGF[beta]1), collagen type 1 (COL1) and osteogenic proteins (ALP, RUNX2) was analyzed by real-time PCR and ELISA. The wound fill rate was examined in an in-vitro wound healing assay. For statistical analyses, Student's t-test and ANOVA were applied (p<0.05). Results: In general, the IL1[beta]-induced expression of proinflammatory mediators was significantly enhanced by CTS on day 1 and significantly downregulated on day 6. CTS of high magnitude significantly inhibited the IGF1 synthesis but significantly upregulated VEGF under normal and inflammatory conditions. In general, CTS also downregulated the IL1[beta]-induced COL1, ALP, and RUNX2 expression. From day 5 on, the lowest wound fill rate was observed in cells which were simultaneously exposed to inflammatory and biomechanical signals. Conclusion: These findings suggest that orthodontic and occlusal loading may contribute to periodontal destruction in periodontally-diseased patients through downregulation of matrix and osteogenic proteins but not via augmentation of periodontal inflammation. Abstract (German): Zusammenfassung Hintergrund und Ziel: Unsachgemass durchgefuhrte kieferorthopadische Zahnbewegungen wie auch okklusale Uberbelastungen konnen eine parodontitisinduzierte Destruktion des Parodontiums verstarken. Die zugrunde liegenden Mechanismen fur den destruktionsverstarkenden Effekt der biomechanischen Belastung sind jedoch noch ungeklart. In dieser In-vitro-Studie sollte untersucht werden, ob biomechanische Krafte die Reaktion von parodontalen Ligament-(PDL-)Zellen auf Entzundungsreize modulieren. Material und Methodik: Auf BioFlex.sup.(r)-Platten kultivierte PDL-Zellen von sechs Patienten wurden mit Interleukin (IL) 1[beta], das an entzundeten parodontalen Stellen erhoht ist, inkubiert und/oder einer zyklischen Zugbelastung (CTS) niedriger (3%) und hoher (20%) Starke fur 1 und 6 Tage ausgesetzt. Die Synthese von proinflammatorischen Mediatoren (IL1[beta], IL8, COX2), Wachstumsfaktoren (IGF1, VEGF, TGF[beta]1), Kollagen Typ 1 (COL1) und osteogenen Proteinen (ALP, RUNX2) wurde mittels Real-Time-PCR und ELISA analysiert. Die Rate der Wundauffullung wurde mit einem In-vitro-Wundheilungsassay untersucht. Fur die statistische Auswertung kamen der Student's t-Test und ANOVA zur Anwendung (p<0,05). Ergebnisse: Im Allgemeinen wurde die IL1[beta]-induzierte Expression der proinflammatorischen Mediatoren durch CTS am Tag 1 signifikant verstarkt und am Tag 6 signifikant gehemmt. CTS hoher Starke reduzierte signifikant die IGF1-Synthese, fuhrte aber zu einer signifikanten Steigerung von VEGF unter normalen und entzundlichen Bedingungen. CTS hemmte im Allgemeinen auch die IL1[beta]-induzierte Expression von COL1, ALP und RUNX2. Ab dem funften Tag wurde die geringste Wundheilungsrate in den Kulturen beobachtet, die gleichzeitig entzundlichen und biomechanischen Signalen ausgesetzt waren. Schlussfolgerung: Diese Ergebnisse legen nahe, dass kieferorthopadische und okklusale Krafte zur parodontalen Destruktion durch Herunterregulation extrazellularer Matrixproteine und osteogener Differenzierungsmarker, jedoch nicht durch Verstarkung der parodontalen Entzundung bei Parodontitispatienten beitragen konnten. Author Affiliation: (1) Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany (2) Department of Periodontology, Operative and Preventive Dentistry Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany (3) Department of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany (4) Oral Technology, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany (5) Clinical Research Unit 208, Department of Periodontology, Operative and Preventive Dentistry Center of Dento-Maxillo-Facial Medicine University of Bonn, Welschnonnenstrasse 17, 53111, Bonn, Germany Article History: Registration Date: 01/01/2010 Received Date: 18/08/2010 Accepted Date: 08/10/2010 Online Date: 17/11/2010
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titleContribution of Orthodontic Load to Inflammationmediated Periodontal Destruction, Beteiligung von kieferorthopädischer Belastung an der entzündungsvermittelten Destruktion des Parodontiums
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