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Selective isolation of hemoglobin by use of imidazolium-modified polystyrene as extractant

Ionic liquids have attracted much attention in the analysis of a variety of species. The functional groups in ionic liquids can result in highly efficient separation and enrichment and, because of their typical lack of volatility, they are environmentally benign. We grafted imidazole cations onto th... Full description

Journal Title: Analytical and Bioanalytical Chemistry 2013, Vol.405(15), pp.5353-5358
Main Author: Zhao, Gang
Other Authors: Chen, Shuai , Chen, Xu-Wei , He, Rong-Huan
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1618-2642 ; E-ISSN: 1618-2650 ; DOI: 10.1007/s00216-013-6889-y
Link: http://dx.doi.org/10.1007/s00216-013-6889-y
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recordid: springer_jour10.1007/s00216-013-6889-y
title: Selective isolation of hemoglobin by use of imidazolium-modified polystyrene as extractant
format: Article
creator:
  • Zhao, Gang
  • Chen, Shuai
  • Chen, Xu-Wei
  • He, Rong-Huan
subjects:
  • Ionic liquid immobilization
  • Protein
  • Adsorption
  • Hemoglobin
  • Biocompatibility
  • Solid-phase extraction
ispartof: Analytical and Bioanalytical Chemistry, 2013, Vol.405(15), pp.5353-5358
description: Ionic liquids have attracted much attention in the analysis of a variety of species. The functional groups in ionic liquids can result in highly efficient separation and enrichment and, because of their typical lack of volatility, they are environmentally benign. We grafted imidazole cations onto the surface of chloromethyl polystyrene, denoted PS-CH 2 -[MIM] + Cl − , and this modified polymer was used to selectively extract the protein hemoglobin (Hb). The prepared extractant PS-CH 2 -[MIM] + Cl − , containing 2 mmol immobilized imidazole groups per gram polymer, was characterized by FT-IR, surface charge analysis, and elemental analysis. The adsorption efficiency was 91 %. The adsorption capacity of the PS-CH 2 -[MIM] + Cl − for Hb was 23.6 μg mg −1 , and 80 % of the retained Hb could be readily recovered by use of 0.5 % ( m / v ) aqueous sodium dodecyl sulfate (SDS) solution as eluate. The activity of the eluted Hb was approximately 90 %. The prepared imidazole-containing solid phase polymer was used for direct adsorption of Hb without use of any other solid matrix as support of the ionic liquid. The material was used in practice to isolate Hb from human whole blood. Figure Coordination interaction between heme of hemoglobin and imidazolium-modified chloromethyl polystyrene.
language: eng
source:
identifier: ISSN: 1618-2642 ; E-ISSN: 1618-2650 ; DOI: 10.1007/s00216-013-6889-y
fulltext: fulltext
issn:
  • 1618-2650
  • 16182650
  • 1618-2642
  • 16182642
url: Link


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titleSelective isolation of hemoglobin by use of imidazolium-modified polystyrene as extractant
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subjectIonic liquid immobilization ; Protein ; Adsorption ; Hemoglobin ; Biocompatibility ; Solid-phase extraction
descriptionIonic liquids have attracted much attention in the analysis of a variety of species. The functional groups in ionic liquids can result in highly efficient separation and enrichment and, because of their typical lack of volatility, they are environmentally benign. We grafted imidazole cations onto the surface of chloromethyl polystyrene, denoted PS-CH 2 -[MIM] + Cl − , and this modified polymer was used to selectively extract the protein hemoglobin (Hb). The prepared extractant PS-CH 2 -[MIM] + Cl − , containing 2 mmol immobilized imidazole groups per gram polymer, was characterized by FT-IR, surface charge analysis, and elemental analysis. The adsorption efficiency was 91 %. The adsorption capacity of the PS-CH 2 -[MIM] + Cl − for Hb was 23.6 μg mg −1 , and 80 % of the retained Hb could be readily recovered by use of 0.5 % ( m / v ) aqueous sodium dodecyl sulfate (SDS) solution as eluate. The activity of the eluted Hb was approximately 90 %. The prepared imidazole-containing solid phase polymer was used for direct adsorption of Hb without use of any other solid matrix as support of the ionic liquid. The material was used in practice to isolate Hb from human whole blood. Figure Coordination interaction between heme of hemoglobin and imidazolium-modified chloromethyl polystyrene.
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abstractIonic liquids have attracted much attention in the analysis of a variety of species. The functional groups in ionic liquids can result in highly efficient separation and enrichment and, because of their typical lack of volatility, they are environmentally benign. We grafted imidazole cations onto the surface of chloromethyl polystyrene, denoted PS-CH 2 -[MIM] + Cl − , and this modified polymer was used to selectively extract the protein hemoglobin (Hb). The prepared extractant PS-CH 2 -[MIM] + Cl − , containing 2 mmol immobilized imidazole groups per gram polymer, was characterized by FT-IR, surface charge analysis, and elemental analysis. The adsorption efficiency was 91 %. The adsorption capacity of the PS-CH 2 -[MIM] + Cl − for Hb was 23.6 μg mg −1 , and 80 % of the retained Hb could be readily recovered by use of 0.5 % ( m / v ) aqueous sodium dodecyl sulfate (SDS) solution as eluate. The activity of the eluted Hb was approximately 90 %. The prepared imidazole-containing solid phase polymer was used for direct adsorption of Hb without use of any other solid matrix as support of the ionic liquid. The material was used in practice to isolate Hb from human whole blood. Figure Coordination interaction between heme of hemoglobin and imidazolium-modified chloromethyl polystyrene.
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pubSpringer Berlin Heidelberg
doi10.1007/s00216-013-6889-y
pages5353-5358
date2013-06