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Fluorescent-protein-based probes: general principles and practices

An important application of fluorescent proteins is to derive genetically encoded fluorescent probes that can actively respond to cellular dynamics such as pH change, redox signaling, calcium oscillation, enzyme activities, and membrane potential. Despite the large diverse group of fluorescent-prote... Full description

Journal Title: Analytical and Bioanalytical Chemistry 2015, Vol.407(1), pp.9-15
Main Author: Ai, Hui-wang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1618-2642 ; E-ISSN: 1618-2650 ; DOI: 10.1007/s00216-014-8236-3
Link: http://dx.doi.org/10.1007/s00216-014-8236-3
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recordid: springer_jour10.1007/s00216-014-8236-3
title: Fluorescent-protein-based probes: general principles and practices
format: Article
creator:
  • Ai, Hui-wang
subjects:
  • Fluorescent protein
  • Förster resonance energy transfer
  • Genetically encoded fluorescent probe
  • Reaction-based fluorescent probe
ispartof: Analytical and Bioanalytical Chemistry, 2015, Vol.407(1), pp.9-15
description: An important application of fluorescent proteins is to derive genetically encoded fluorescent probes that can actively respond to cellular dynamics such as pH change, redox signaling, calcium oscillation, enzyme activities, and membrane potential. Despite the large diverse group of fluorescent-protein-based probes, a few basic principles have been established and are shared by most of these probes. In this article, the focus is on these general principles and strategies that guide the development of fluorescent-protein-based probes. A few examples are provided in each category to illustrate the corresponding principles. Since these principles are quite straightforward, others may adapt them to create fluorescent probes for their own interest. Hopefully, the development of the ever-growing family of fluorescent-protein-based probes will no longer be limited to a small number of laboratories specialized in senor development, leading to the situation that biological studies will be bettered assisted by genetically encoded sensors. Graphical Abstract ᅟ
language: eng
source:
identifier: ISSN: 1618-2642 ; E-ISSN: 1618-2650 ; DOI: 10.1007/s00216-014-8236-3
fulltext: fulltext
issn:
  • 1618-2650
  • 16182650
  • 1618-2642
  • 16182642
url: Link


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subjectFluorescent protein ; Förster resonance energy transfer ; Genetically encoded fluorescent probe ; Reaction-based fluorescent probe
descriptionAn important application of fluorescent proteins is to derive genetically encoded fluorescent probes that can actively respond to cellular dynamics such as pH change, redox signaling, calcium oscillation, enzyme activities, and membrane potential. Despite the large diverse group of fluorescent-protein-based probes, a few basic principles have been established and are shared by most of these probes. In this article, the focus is on these general principles and strategies that guide the development of fluorescent-protein-based probes. A few examples are provided in each category to illustrate the corresponding principles. Since these principles are quite straightforward, others may adapt them to create fluorescent probes for their own interest. Hopefully, the development of the ever-growing family of fluorescent-protein-based probes will no longer be limited to a small number of laboratories specialized in senor development, leading to the situation that biological studies will be bettered assisted by genetically encoded sensors. Graphical Abstract ᅟ
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abstractAn important application of fluorescent proteins is to derive genetically encoded fluorescent probes that can actively respond to cellular dynamics such as pH change, redox signaling, calcium oscillation, enzyme activities, and membrane potential. Despite the large diverse group of fluorescent-protein-based probes, a few basic principles have been established and are shared by most of these probes. In this article, the focus is on these general principles and strategies that guide the development of fluorescent-protein-based probes. A few examples are provided in each category to illustrate the corresponding principles. Since these principles are quite straightforward, others may adapt them to create fluorescent probes for their own interest. Hopefully, the development of the ever-growing family of fluorescent-protein-based probes will no longer be limited to a small number of laboratories specialized in senor development, leading to the situation that biological studies will be bettered assisted by genetically encoded sensors. Graphical Abstract ᅟ
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doi10.1007/s00216-014-8236-3
pages9-15
date2015-01