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Microsatellite typing of the rhesus macaque MHC region

To improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu - DRB loci, the Mamu - A and - B are not only polymorphic but also polygenic. As a cons... Full description

Journal Title: Immunogenetics 2005, Vol.57(3), pp.198-209
Main Author: Penedo, M.
Other Authors: Bontrop, Ronald , Heijmans, Corrine , Otting, Nel , Noort, Riet , Rouweler, Annemiek , Groot, Nanine , Groot, Natasja , Ward, Thea , Doxiadis, Gaby
Format: Electronic Article Electronic Article
Language: English
Subjects:
MHC
ID: ISSN: 0093-7711 ; E-ISSN: 1432-1211 ; DOI: 10.1007/s00251-005-0787-1
Link: http://dx.doi.org/10.1007/s00251-005-0787-1
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recordid: springer_jour10.1007/s00251-005-0787-1
title: Microsatellite typing of the rhesus macaque MHC region
format: Article
creator:
  • Penedo, M.
  • Bontrop, Ronald
  • Heijmans, Corrine
  • Otting, Nel
  • Noort, Riet
  • Rouweler, Annemiek
  • Groot, Nanine
  • Groot, Natasja
  • Ward, Thea
  • Doxiadis, Gaby
subjects:
  • MHC
  • Non-human primates
  • Evolution
  • Microsatellites
  • Haplotype
ispartof: Immunogenetics, 2005, Vol.57(3), pp.198-209
description: To improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu - DRB loci, the Mamu - A and - B are not only polymorphic but also polygenic. As a consequence, sequence-based typing of these genes is time-consuming. Therefore, eight MHC-linked microsatellites, or short tandem repeats (STRs), were evaluated for their use in haplotype characterization. Polymorphism analyses in rhesus macaques of Indian and Chinese origin showed high STR allelic diversity in both populations but different patterns of allele frequency distribution between the groups. Pedigree data for class I and II loci and the eight STRs allowed us to determine extended MHC haplotypes in rhesus macaque breeding groups. STR sequencing and comparisons with the complete rhesus macaque MHC genomic map allowed the exact positioning of the markers. Strong linkage disequilibria were observed between Mamu - DR and - DQ loci and adjacent STRs. Microsatellite typing provides an efficient, robust, and quick method of genotyping and deriving MHC haplotypes for rhesus macaques regardless of their geographical origin. The incorporation of MHC-linked STRs into routine genetic tests will contribute to efforts to improve the genetic characterization of the rhesus macaque for biomedical research and can provide comparative information about the evolution of the MHC region.
language: eng
source:
identifier: ISSN: 0093-7711 ; E-ISSN: 1432-1211 ; DOI: 10.1007/s00251-005-0787-1
fulltext: fulltext
issn:
  • 1432-1211
  • 14321211
  • 0093-7711
  • 00937711
url: Link


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titleMicrosatellite typing of the rhesus macaque MHC region
creatorPenedo, M. ; Bontrop, Ronald ; Heijmans, Corrine ; Otting, Nel ; Noort, Riet ; Rouweler, Annemiek ; Groot, Nanine ; Groot, Natasja ; Ward, Thea ; Doxiadis, Gaby
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subjectMHC ; Non-human primates ; Evolution ; Microsatellites ; Haplotype
descriptionTo improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu - DRB loci, the Mamu - A and - B are not only polymorphic but also polygenic. As a consequence, sequence-based typing of these genes is time-consuming. Therefore, eight MHC-linked microsatellites, or short tandem repeats (STRs), were evaluated for their use in haplotype characterization. Polymorphism analyses in rhesus macaques of Indian and Chinese origin showed high STR allelic diversity in both populations but different patterns of allele frequency distribution between the groups. Pedigree data for class I and II loci and the eight STRs allowed us to determine extended MHC haplotypes in rhesus macaque breeding groups. STR sequencing and comparisons with the complete rhesus macaque MHC genomic map allowed the exact positioning of the markers. Strong linkage disequilibria were observed between Mamu - DR and - DQ loci and adjacent STRs. Microsatellite typing provides an efficient, robust, and quick method of genotyping and deriving MHC haplotypes for rhesus macaques regardless of their geographical origin. The incorporation of MHC-linked STRs into routine genetic tests will contribute to efforts to improve the genetic characterization of the rhesus macaque for biomedical research and can provide comparative information about the evolution of the MHC region.
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titleMicrosatellite typing of the rhesus macaque MHC region
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abstractTo improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu - DRB loci, the Mamu - A and - B are not only polymorphic but also polygenic. As a consequence, sequence-based typing of these genes is time-consuming. Therefore, eight MHC-linked microsatellites, or short tandem repeats (STRs), were evaluated for their use in haplotype characterization. Polymorphism analyses in rhesus macaques of Indian and Chinese origin showed high STR allelic diversity in both populations but different patterns of allele frequency distribution between the groups. Pedigree data for class I and II loci and the eight STRs allowed us to determine extended MHC haplotypes in rhesus macaque breeding groups. STR sequencing and comparisons with the complete rhesus macaque MHC genomic map allowed the exact positioning of the markers. Strong linkage disequilibria were observed between Mamu - DR and - DQ loci and adjacent STRs. Microsatellite typing provides an efficient, robust, and quick method of genotyping and deriving MHC haplotypes for rhesus macaques regardless of their geographical origin. The incorporation of MHC-linked STRs into routine genetic tests will contribute to efforts to improve the genetic characterization of the rhesus macaque for biomedical research and can provide comparative information about the evolution of the MHC region.
copBerlin/Heidelberg
pubSpringer-Verlag
doi10.1007/s00251-005-0787-1
pages198-209
date2005-05